Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Effects of Isoflavones Intake Level on Bone Markers and Bone Related Hormones in Growing Female Rats

이소플라본 섭취수준이 성장기 암컷 쥐의 골대사지표 및 골대사관련호르몬에 미치는 영향

  • Choi, Mi-Ja (Department of Food and Nutrition, Keimyung University) ;
  • Jung, Yun-Jung (Department of Food and Nutrition, Keimyung University)
  • 최미자 (계명대학교 식품영양학과) ;
  • 정윤정 (계명대학교 식품영양학과)
  • Published : 2008.04.30
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Abstract

The overall purpose of this study was to investigate the effects of level of isoflavones supplementation on bone metabolism in growing female rats. Forty-five rats divided into three groups; Control, l/2IF, and lIF. Serum osteocalcin and alkaline phosphatase (ALP) activity, urinary deoxypyridinoline (DPD) crosslinks value were measured to monitor bone formation and resorption at the ninth week after feeding. Hormones related to bone metabolism were determined, included parathyroid hormone (PTH) , calcitonin, estradiol, growth hormone and insulin-like growth factor I (IGF-I). The results of this study were as follows: the isoflavones intake level did not affect weight gain, mean food intake and food efficiency ratio. The serum concentration of osteocalcin and the activity ofALP were not significantly different by different levels of isoflavones supplementation. The urinary DPD crosslinks value was not significantly different by different levels ofisoflavones supplementation. There were no significant differences in serum PTH, estradiol and IGFI among all groups. However, calcitonin was shown significantly higher in the groups of lIF and l/2IF than control group. And growth hormone was shown significantly higher in the groups of lIF than control group. (Korean J Nutr 2008; 41(3): 199~205)

성장기 암컷 쥐를 대상으로 이소플라본 섭취수준에 따라 골대사 지표 및 골대사 관련 호르몬에 미치는 영향을 알아 보기 위해 실시한 연구 결과를 요약하면 다음과 같다. 1) 체중증가량과 식이섭취량, 식이효율은 이소플라본의 섭취수준에 따라 실험군간에 유의적인 차이가 없었다. 2) 골형성 지표인 혈청 ALP, osteocalcin은 이소플라본 섭취수준에 따라 실험군간에 유의적인 차이는 없었다. 3) 골흡수 지표인 DPD crosslink value도 이소플라본 섭취수준에 따라 실험군간에 유의적인 차이는 없었다. 4) 혈청 에스트로겐 농도, 부갑상선 호르몬과 인슐린유사 성장인자-I 농도는 이소플라본 섭취수준에 따라 유의적인 차이가 없었다. 5) 칼시토닌농도는 1IF군과 1/2IF군 보다 1IF군이 골형성과 골흡수에 유리한 경향을 나타내었고 칼시토닌과 성장호르몬이 유의적으로 증가하여 장기적으로는 골대사에 더 유리한 영향을 미칠 것으로 사료된다.

Keywords

References

  1. Kuiper GG, Lemmen JG, Carlsson B, Corton JC, Safe SH, Saag PT, Burg B, Gustafsson JA. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor $\beta$. Endocrinology 1998; 139(10): 4252-4263 https://doi.org/10.1210/en.139.10.4252
  2. Messina ML. Soy foods and soybean isoflavone and menopausal health. Nutr Clin Care 2002; 5(6): 272-282 https://doi.org/10.1046/j.1523-5408.2002.05602.x
  3. Messina M, Messina V. Soyfoods, soybean isoflavones, and health: a brief overview. J Ren Nutr 2000; 10(2): 63-68 https://doi.org/10.1016/S1051-2276(00)90001-3
  4. Coward L, Barnes NC, Setchell KDR, Barnes S. Genistein, daidzein, and their $\beta$-glycoside conjugates: Antitumor isoflovones in soybean foods from American and Asian diets. J Agric Food Chem 1993; 41: 1981-1987
  5. Kwon TW, Song YS, Kim TS, Moon GS, KIm JI, Hong JH. Current research on the bioactive functions of soy foods in Korea. Korea Soybeen Digest 1998; 15: 147-160
  6. Lee MJ, Kim MJ, Min SH, Yoon S. A study on attitude of soy food and estimated dietary isoflavone intake among Korean adolescents. Korean J Community Nutrition 2004; 9(5): 606-614
  7. Greendale GA, Fitzgerald G, Huang MH, Sternfeld B, Gold E, Seeman T, Sherman S, Sowers M. Dietary soy isoflavones and bone mineral density: Results from the study of women's health across the nation. Am J Epidemiol 2002; 155: 746-754 https://doi.org/10.1093/aje/155.8.746
  8. Anderson JJB, Garner SC. The effects of phytoestrogens on bone. Nutrition Research 1997; 17(10): 1617-1632 https://doi.org/10.1016/S0271-5317(97)00156-5
  9. Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JJW. Soy protein and isoflavones: Their effects on blood lipids and bone density in postmenopausal women. Am J Clin Nutr 1998; 68(suppl): 1375s-1379s https://doi.org/10.1093/ajcn/68.6.1375S
  10. Picherit C, Chanteranne B, Pelissero CB, Davicco MJ, Lebecque P, Barlet JP, Coxam V. Does-dependent bone-sparing effects of dietary isoflavones in the ovariectomized rats. Br J Nutr 2001; 85: 307-316 https://doi.org/10.1079/BJN2000252
  11. Picherit C, Bennetau-Pelissero C, Chanternne B, Lebecque P, Cavicco MJ, Barlet JP, Coxam V. Soybean isoflavones dose-dependently reduce bone turnover but do not reverse established osteopenia in adult ovariectomized rats. J Nutr 2001; 131: 723-728 https://doi.org/10.1093/jn/131.3.723
  12. Choi MJ, Jung YJ. The effects of level of isoflavones supplementation on bone mineral density in growing female rat. Korean J Nutr 2006; 39(4): 338-346
  13. Choi MJ, Jo HJ. Effects of soy and isoflavones on bone metabolism in growing female rat. Korean J Nutr 2003; 36(6): 549-558
  14. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents. J Nutr 1993; 123: 1939-1951 https://doi.org/10.1093/jn/123.11.1939
  15. Tietz NW. Alkaline phosphatase study group, Committee on standards of the AACC, Subcommittee on enzymes. Progress in the development of a recommended method for alkaline phosphatase activity measurements. Clin Chem 1980; 26: 1023-1027
  16. Nanda N, Joshi H, Subbarao SK, Sharma VP. Two-site immunoradiometricassay (IRMA): detection, efficiency, and procedural modifications. J Am Mosq Control Assoc 1994; 10: 225-227
  17. Xing S, Cekan SZ, Dicafalusy U. Validation of radioimunoassay for 17-estradiol isotope dilution-mass spectrometry and a test of radiochemical purity. Clin Chem Acta 1983; 135: 189-201 https://doi.org/10.1016/0009-8981(83)90135-3
  18. Guarnero P, Grimaux M, Seguin P, Delmas P. Characterization of immunoreactive forms of human osteocalcin generated in vivo and in vitro. J Bone Min Res 1994; 9: 692-698
  19. Arjmandi BH, Birnbaum R, Goyal NV, Getlinger MJ, Juma S, Alekel L, Hasler CM, Drum ML, Hollis BW, Kukreja SC. Bonesparing effect of soy protein in ovarian hormone-deficient rats is related to its isoflavone content. Am J Clin Nutr 1998; 68(suppl): 1364s-1368s https://doi.org/10.1093/ajcn/68.6.1364S
  20. Chen YM, Ho SC, Lam SSH, Ho SSS, Woo SLF. Soy isoflavones have a favorable effect on bone loss in Chinese postmenopausal women with lower bone mass: A double-blind, randomized, controlled trial. J Clin Endocrinol Metab 2003; 88 (10): 4740-4747 https://doi.org/10.1210/jc.2003-030290
  21. Arjmandi BA, Alekel L, Hollis BW, Amin D, Stacewicz-Sapuntzakis M, Guo P, and Jukreja SC. Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporoses. J Nutr 1996; 126: 161-167 https://doi.org/10.1093/jn/126.1.161
  22. Picherit C, Coxam V, Bennetau-Pelissero C, Kati-Coulibaly S, Davicco MJ, Lebecque P, Barlet JP. Daidzein is more efficient than genistein in preventing ovariectomy-induced bone loss in rats. J Nutr 2000; 130: 1675-1681 https://doi.org/10.1093/jn/130.7.1675
  23. King RA. Daidzein conjugates are more bioavailable than genistein conjugates in rat. Am J Clin Nutr 1998; 68: 1496s-1499s https://doi.org/10.1093/ajcn/68.6.1496S
  24. Wangen KE, Duncan AM, Merz-demlow BE, Xu X, Marcus R, Phipps WR, Kurzer MS. Effects of soy isoflavones on markers of bone turnover in premenopausal and postmenopausal women. J Clin Endocrinol Metab 2000; 85(9): 3043-3048 https://doi.org/10.1210/jc.85.9.3043
  25. Jeon HS. Effects of soy protein and isoflavones on bone mineral content and bone mineral density in growing rats consuming a low calcium diet [MS Thesis]. Daegu: Keimyung University; 2003
  26. Compston JE. Sex steroids and bone. Physiol Rev 2001; 81: 419-447 https://doi.org/10.1152/physrev.2001.81.1.419
  27. Kurzer MS, Xu X. Dietary phytoestrogens. Annu Rev Nutr 1997; 17: 353-381 https://doi.org/10.1146/annurev.nutr.17.1.353
  28. Hedlund LR, Gallagher JC. The effect of age and menopause on bone mineral density of proximal femur. J Bone Miner Res 1989; 4(4): 639-642 https://doi.org/10.1002/jbmr.5650040423
  29. Xu X, Wang HJ, Murphy PA, Hendrich S. Neither background diet nor type of soy food affects short-term isoflavone bioavailability in women. J Nutr 2000; 130: 798-801 https://doi.org/10.1093/jn/130.4.798
  30. Nagata C, Inaba S, Kawakami N, Kakizoe T, Shimizu H. Inverse association of soy product intake with serum androgen and estrogen concentrations in Japanese men. Nutr Cancer 2000; 36(1): 14-18 https://doi.org/10.1207/S15327914NC3601_3
  31. Won HJ, Lee BS, Lee SK, Choi Y, Yoon S, Park KH. The effects of isoflavone on postmenopausal symptoms and hormonal changes in postmenopausal women. Korean Society of Menopause 2001; 7(1): 54-63
  32. Gruber HF, Ivey JL, Baylink DJ. Long Term Calcitonin Therapy in postmenopausal osteoporosis. Metabolism 1984; 33: 295-303 https://doi.org/10.1016/0026-0495(84)90187-2
  33. Chenu D, Valentin-Opran A, Chavassieux P, Saez S, Meunier PJ, Delmas PD. Insulin like growth factor I hormonal regulation by growth hormone and by 1,24$(OH)_2D_3$ and activity on human osteoblast- like cells in short-term cultures. Bone 1990; 11(2): 81-86 https://doi.org/10.1016/8756-3282(90)90054-3
  34. Canalis E. Skeletal growth factors and aging. J Clin Endocrinol Metab 1994; 78(5): 1009-1010 https://doi.org/10.1210/jc.78.5.1009
  35. Kurland ES, Rosen CJ, Cosman F, Mcmahon D, Chan F, Shane E, Lindsay R, Dempster D, Bilezikian JP. Insulin-like growth factor-I in men with idiopathic osteoporosis. J Clin Endocrinol Metab 1997; 82: 2799-2805 https://doi.org/10.1210/jc.82.9.2799
  36. Kassem M, Blum W, Risteli J. Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro. Calcif Tissue Int 1993; 52: 222-226 https://doi.org/10.1007/BF00298723
  37. Hartmann B, Kirchengast S, Albrecht A, Laml T, Bikas D, Huber J. Effects of hormone replacement therapy on growth hormone secretion patterns in correlation to somatometric parameters in healthy postmenopausal women. Maturitas 1995; 22: 239-246 https://doi.org/10.1016/0378-5122(95)00942-E
  38. Jo HJ. The effects of soy protein and soy isoflavones on bone and lipid metabolism in growing female rats [Dissertation]. Daegu: Keimyung University; 2002