Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Mycological Characteristics of Botrytis cinerea Causing Gray Mold on Ginseng in Korea

인삼 잿빛곰팡이병균 Botrytis cinerea의 균학적 특성

  • Cho, Hye-Sun (Bio-resources Center, KT&G Central Research Institute) ;
  • Jeon, Yong-Ho (Bio-resources Center, KT&G Central Research Institute) ;
  • Do, Gyung-Ran (National Horticultural Research Institute, Rural Development Administration) ;
  • Cho, Dae-Hui (Bio-resources Center, KT&G Central Research Institute) ;
  • Yu, Yun-Hyun (Bio-resources Center, KT&G Central Research Institute)
  • 조혜선 (KT&G 중앙연구원 생물자원연구소) ;
  • 전용호 (KT&G 중앙연구원 생물자원연구소) ;
  • 도경란 (농촌진흥청 원예연구소) ;
  • 조대휘 (KT&G 중앙연구원 생물자원연구소) ;
  • 유연현 (KT&G 중앙연구원 생물자원연구소)
  • Published : 2008.03.31
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Abstract

A total of 29 Botrytis were isolated from ginseng gray mold at 8 locations in Korea from June to July, 2004 and 2005. The causative agent of them was identified as Botrytis cinerea based on morphological characteristics of conidia and RPB2 gene sequence analysis. In inoculation experiments with sclerotia, one isolate was pathogenic to ginseng plant whereas two isolates were non-pathogenic. Scanning electron microscopy revealed that the sclerotia of pathogenic isolate has rough surface and that of non-pathogenic isolate very smooth surface. Optimum temperature and culture conditions for sclerotia production were $5{\sim}20^{\circ}C$ and darkness, respectively. The number of sclerotia was increased tenfold on media added with 0.5% old stem fragment of ginseng.

인삼 고년생 결주의 주원인으로 작용하는 잿빛곰팡이병 방 제을 위한 기초 연구로 병원균을 분리, 동정하고 이들의 균학 적 특성을 구명하였다. 잿빛곰팡이병반으로부터 균을 분리하 여 분생포자의 형태적 특징을 관찰하고 RPB2 유전자의 염기 서열 분석을 실시한 결과 분리균은 Botrytis cinerea로 동정되 었다. 균핵을 이용한 병원성 검정 결과 공시한 3균주 중 1균 주는 병원성, 2 균주는 비병원성을 나타내었다. 주사전자현미 경으로 균핵을 관찰한 결과 병원성 균주는 표면이 주름지고 거친 반면 비병원성 균주는 매끈하였다. 균핵 형성은 $5{\sim}20^{\circ}C$, 암 상태에서 양호하였으며, 0.5% 고죽을 첨가한 배 지에서 10배 정도 증가하였다.

Keywords

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