YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Reactivity of Prototype Foamy Virus Integrase to the Mutants of the Highly Conserved Terminal Sequence of U5 LTR

원조포미바이러스 U5 LTR 말단의 보존적인 잔기의 돌연변이에 대한 인테그라제의 반응성

  • Hyun, U-Sok (Department of Biotechnology, Chung-Ang University) ;
  • Lee, Dong-Hyun (Department of Biotechnology, Chung-Ang University) ;
  • Ko, Hyun-Tak (Department of Biotechnology, Chung-Ang University) ;
  • Shin, Cha-Gyun (Department of Biotechnology, Chung-Ang University)
  • 현우석 (중앙대학교 산업과학대학 생명공학과) ;
  • 이동현 (중앙대학교 산업과학대학 생명공학과) ;
  • 고현탁 (중앙대학교 산업과학대학 생명공학과) ;
  • 신차균 (중앙대학교 산업과학대학 생명공학과)
  • Published : 2008.04.30
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Abstract

The long terminal repeat (LTR) of retroviral DNA genome plays an important role in the integration process by providing substrate recognition site for viral integrase (IN). The dinucleotide CA near the 3'-end of the LTR termini is completely conserved among retoviruses. In order to study specificity of interaction between prototype foamy virus (PFV) IN and its U5 LTR DNA, the effect of mutagenesis of the CA sequence was investigated by studying reactivity of PFV IN to the mutant LTR substrates. Replacement of only the C or the A allowed 60 to 100% of the reactivity of the wild type LTR substrate. In addition, replacement of the C and the A showed 50 to 80% of the reactivity of the wild type LTR substrate, indicating that PFV IN has less specificity on the conserved CA sequence when it is compared to the other retroviral INs. Therefore it is suggested that PFV IN is less dependent on the conserved sequence of LTR termini for its enzymatic reaction.

Keywords

References

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