The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Antimicrobial Effect on the Periodontal Pathogens and Anti-inflammatory Effect of Eriobotryae Folium

비파엽(枇杷葉)의 치주염세균에 대한 항균효과 및 항염효과

  • Jeong, Mi-Young (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute) ;
  • Kim, Young-Hong (KyungHee University, Department of Meridian and Acupuncture, College of Eastern Medicine) ;
  • Lee, Na-Kyung (KyungHee University, Department of Meridian and Acupuncture, College of Eastern Medicine) ;
  • Lee, Jin-Yong (Kyung Hee University, Department of Oral Microbiology, College of Dentistry) ;
  • Herr, Yeek (Kyung Hee University, Department of Oral Microbiology, College of Dentistry) ;
  • Lee, Je-Hyun (Kyung Hee University, Department of Korean Medicine Dongguk University) ;
  • Lim, Sa-Bi-Na (WHO Collaborating Center for Traditional Medicine, East-West Medical Research Institute, KyungHee University, Department of Meridian and Acupuncture, College of Eastern Medicine)
  • 정미영 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소) ;
  • 김영홍 (경희대학교 한의과대학 경혈학교실) ;
  • 이나경 (경희대학교 한의과대학 경혈학교실) ;
  • 이진용 (경희대학교 치과대학 구강미생물학교실) ;
  • 허익 (경희대학교 치과대학 치주과학교실) ;
  • 이제현 (동국대학교 한의과대학 본초학교실) ;
  • 임사비나 (경희대학교 WHO 전통의학연구협력센터 동서의학연구소, 경희대학교 한의과대학 경혈학교실)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The purpose of this study was to evaluate on the antimicrobial effect on the periodontal pathogens and anti-inflammatory effect of Eriobotryae folium. Eriobotryae folium are constituent herbs of Gagamgamroum, which has been used for a long time in oriental medicine as a herbal medicine for treating halitosis and toothache. Method: Eriobotryae folium was prepared by extracting medicinal herb with water. We investigated antimicrobial activity by the minimum inhibitory concentration (MIC) test. We also investigated inhibition of $IL-1{\beta}-induced$ collagenase (mmp-1), stromelysin-1 (mmp-3), interleukin-6 gene expression in human gingival fibroblasts using RTPCR analysis. Result: The antimicrobial effects of Eriobotryae folium was evaluated with MIC against periodontopathogens; Porphyromonas gingivalis 2561, W50, A7A1-28, 9-14K-1, Prevotella intermedia 28, and Actinobacillus actinomycetemcomitans Y4. MICs of Eriobotryae folium were 1.25 mg/ml, 2.5 mg/ml, 0.625 mg/ml, 1.25 mg/ml, 10 mg/ml and 10 mg/ml. The anti-inflammatory effect of Eriobotryae folium was evaluated with influence of herbs on the $IL-1{\beta}-induced$ expression of mmp-1, mmp-3, and interleukin-6. $IL-1{\beta}$ increased mmp-1, mmp-3, and interleukin-6 mRNA levels. Eriobotryae folium significantly inhibited $IL-1{\beta}-induced$ mmp-1, mmp-3, and interleukin-6 gene expressions in a dose-dependent manner. Conclusion: These results suggested that Eriobotryae folium might reduce the excessive proteolytic capacity of the gingival fibroblast during inflammation and could be developed as a new drug for periodontitis.

Keywords