Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bacillus anthracis Spores Influence ATP Synthase Activity in Murine Macrophages

  • Seo, Gwi-Moon (Division of Molecular and Life Sciences, Hanyang University) ;
  • Jung, Kyoung-Hwa (Division of Molecular and Life Sciences, Hanyang University) ;
  • Kim, Seong-Joo (Division of Molecular and Life Sciences, Hanyang University) ;
  • Kim, Ji-Cheon (Division of Molecular and Life Sciences, Hanyang University) ;
  • Yoon, Jang-Won (Division of Molecular and Life Sciences, Hanyang University) ;
  • Oh, Kwang-Keun (Department of Bioprocess Technology, BioPolytechnic College) ;
  • Lee, Jung-Ho (Department of Chemical Engineering, Hanyang University) ;
  • Chai, Young-Gyu (Division of Molecular and Life Sciences, Hanyang University)
  • Published : 2008.04.30
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Abstract

Anthrax is an infectious disease caused by toxigenic strains of the Gram-positive bacterium Bacillus anthracis. To identify the mitochondrial proteins that are expressed differently in murine macrophages infected with spores of B. anthracis Sterne, proteomic and MALDI-TOF/MS analyses of uninfected and infected macrophages were conducted. As a result, 13 mitochondrial proteins with different expression patterns were discovered in the infected murine macrophages, and some were identified as ATP5b, NIAP-5, ras-related GTP binding protein B isoform CRAa, along with several unnamed proteins. Among these proteins, ATP5b is related to energy production and cytoskeletal rearrangement, whereas NIAP-5 causes apoptosis of host cells due to binding with caspase-9. Therefore, this paper focused on ATP5b, which was found to be down regulated following infection. The downregulated ATP5b also reduced ATP production in the murine macrophages infected with B. anthracis spores. Consequently, this study represents the first mitochondrial proteome analysis of infected macrophages.

Keywords

References

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