Analysis of Components in the Different Parts of Ailanthus altissima

가죽나무(Ailanthus altissima)의 부위별 성분 분석

  • Lee, Yang-Suk (Department of Herbal Biotechnology, Daegu Haany University)
  • 이양숙 (대구한의대학교 한방생약자원학과)
  • Published : 2008.04.28

Abstract

This study was carried out to analyze the components of the roots, stems, and leaves of Ailanthus altissima to obtain basic data on the nutritional and functional materials developed a functional food with A. altissima. Among the general components, crude ash (9.20%) in the roots, crude protein (11.36%) in leaves, and carbohydrates (81.74%) in stems were higher than other parts. The content of soluble protein was the highest 9,839.52 mg% in leaves. Reducing sugar and free sugar in roots were 1,813.94 mg% 1,140.20 mg% and 1,670.98 mg% 1,190.42 mg% in leaves, respectively. The contents of free amino acid (2,018.58 mg%) in roots were higher than leaves (1,070.88 mg%) and stems (427.55 mg%). Especially arginine (1,446.63 mg%) and aspartic acid (252.82 mg%) in roots were the highest. Total content of amino acid derivatives were 780.70 mg% in leaves and 430.95 mg% in roots. Especially, taurine was 61.68 mg% in roots. In the results of mineral analysis, the contents of Ca, K and Mg which account for 83% to 98% of mineral contents, were high in all parts. The polyphenol compounds in leaves and water extract of leaves were 821.58 mg% and 8,040.35 mg%. And contents of flavonoids were 2,501.67 mg% in leaves and 13,592.20 mg% in ethanol extract of leaves, respectively.

본 연구는 뿌리를 저근백피라는 한방생약재로 사용하는 가죽나무(Ailanthus altissima)의 뿌리와 줄기 그리고 잎을 기능성 식품을 개발하기 위한 연구의 일환으로 영양정분과 생리활성 물질의 함유량을 측정하였다. 일반성분을 분석한 결과 뿌리에는 조회분(9.20%)과 조단백질(7.86%)이 많았으며, 잎에는 조단백질(11.36%) 그리고 줄기에서는 탄수화물(81.74%)의 함유량이 비교적 높았다. 수용성 단백질은 잎에서 9,839.52 mg%로 뿌리와 줄기보다 매우 많이 함유하였으며, 환윈당과 유리당은 뿌리에서 1,813.94 mg%와 1,140.20 mg%, 잎에서는 1,670.98 mg%와 1,190.42 mg%를 함유하였다. 유리아미노산은 뿌리에서 2,018.58 mg%로 잎(1,070.88 mg%)과 줄기(427.55 mg%)보다 많이 함유하였으며, 특히 뿌리에는 arginine과aspartic acid의 함유량이 매우 높아 유리아미노산의 약 85%를 차지하였다. 아미노산 유도체 함량은 잎에서 780.70 mg%로 가장 높았으며, 뿌리는 430.95 mg%을 함유하였다. 이중 뿌리에는 항산화 및 간기능 향상에 효과가 있는 것으로 알려진 taurine을 61.68 mg% 함유하였다. 가죽나무의 부위별 무기질을 분석한 결과 모든 부위에서 Ca와 K 그리고 Mg의 함유량이 매우 높아 무기질 총량의 $83{\sim}98%$를 차지하였으며, 뿌리에는 Fe와 Al의 함량도 비교적 높았다. 가죽나무의 부위별 시료와 추출물에 대한 폴리페놀과 플라보노이드 화합물을 측정한 결과, 잎에는 821.58 mg%의 폴리페놀과 2,501.67 mg%의 플라보노이드를 함유하였으며, 추출물 또한 잎의 물 추출물에서 8,040.35 mg%의 폴리페놀과 에탄을 추출물에서 13,592.20mg%의 플라보노이드를 함유하였다. 이상의 결과 한방생약재로 사용되고 있는 가죽나무의 뿌리를 포함하여 잎에서도 영양성분과 생리활성 물질이 다량 함유된 것으로 분석되었다.

Keywords

References

  1. Hatano, T. (1995) Constituents of natural medicines with scavenging effect on active oxygen species-tannins and related polyphenols. Nat. Med., 49, 357-363
  2. Heo, S.J., Yang, M.Y. and Cho, E.J. (2001) Analysis of Umbelliferaceae wild plants and antioxidative activity of pork meat products added with wild plants. Korean J. Soc. Food Cookery Sci., 17, 456-463
  3. 최영전. (1992) 산나물 재배와 이용법. 오성출판사, 서울, p.206
  4. Lee, T.B. (1993) Illustrated flora of Korea. 5th ed. Hyangmoonsa, Seoul, Korea. p.505-506
  5. Kim, T.W. (1996) The woody plants of Korea in color 4th. Kyo-Hak Publishing Co., p.485-486
  6. 江蘚新醫學院. (1979) 中葯大辭典. 1th. 下冊, 上海, 中國, p. 2577-2579
  7. 구본홍. (1994) 동의보감 한글완역본(허준 저). 대중서관, p. 555, 1456
  8. 國家中医藥管理局編委會. (1999) 中華本草. 上海科學技術出版社 上海, 中國, 5, 3-6
  9. Barakat, H.H. (1998) Chemical investigation of the constitutive phenolics of the structure of a new flavone glycoside gallate. Nat. Prod. Sci., 4, 153-157
  10. Kazuya, K., Katsuyoshi, M., Kazuo, K. and Taichi, O. (1994) Studies on the constituents of Ailanthus integrifolia. Chem. Pharm. Bull., 42, 1669-1671 https://doi.org/10.1248/cpb.42.1669
  11. Lee, Y.S. (2007) Physiological activities of hot water extract from Ailanthus altissima. Korean J. Food Preserv., 14, 170-176
  12. Lee, D.G., Chang, Y.S., Park, Y.K., Hahm, K.S. and Woo, E.R. (2002) Antimicrobial effects of ocotillone isolated from stem bark of Ailanthus altisshima. J. Microbiol. Biotechnol., 12, 854-857
  13. Kim, K.W., Baek, J.K., Jang, Y.W., Kim, E.J., Kwon, Y.S., Li, H.J. and Sohn, H.Y. (2005) Screening of antibacterial agent against Streptococcus mutants from natural and medicinal plants. J. Life Sci., 15, 715-725 https://doi.org/10.5352/JLS.2005.15.5.715
  14. Kubota, K., Fukamiya, N., Tokuda, H., Nishino, H., Tagahara, K., Lee, K.H. and Okano, M. (1997) Quassinoids as inhibitors of epstein-barr virus early antigen activation. Cancer Lett., 113, 165-168 https://doi.org/10.1016/S0304-3835(97)04607-7
  15. Jeong, Y.M., Park, S.K., Lee, K.J., Kim, Y.M., Yun, Y.G., Kim, W.S., Han, D.M., An, W.G., Yoon, Y.S. and Jeon, B.H. (2003) Effect of Ailanthus altissima on the apoptosis and cell cycle of HL-60 leukemia cell line. Korean J. Ori. Physiol, Pathol., 17, 914-922
  16. Hwang, W.G., Lee, H.C., Kim, C.K., Kim, D.G., Lee, G.O., Yun, Y.G. and Jeon, B.H. (2002) Effect of Ailanthus altissima water extract on cell cycle control genes in Jurkat T lymphocytes. Yakhak Hoeji, 46, 18-23
  17. Kim, J., Kim, H.K., Park, S.W., Choi, J.W. and Lee, C.K. (1994) Studies on the biological activities of the constituents of Ailanthi cortex radicis II. Acute and renal toxicity of chloroform fraction on epoxide hydrolyzing system in liver. Kor. J. Pharmacogn., 25, 47-50
  18. Pascual-villalobos, M.J. and Robledo, A. (1998) Screening for anti-insect activity in mediterranean plants. Industr. Crops Prod., 8, 183-194 https://doi.org/10.1016/S0926-6690(98)00002-8
  19. A.O.A.C. (1995) Official method of analysis. 18th ed., Association of official analytical chemists. Washington, USA, p.210-219
  20. Lowry, O.H., Roserbrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275
  21. Nelson, N. (1944) A photometric adaption of the somogyi method for determination of glucose. J. Biol. Chem., 153, 375-381
  22. Shim, K.H., Sung, N.K., Choi, J.S. and Kang, K.S. (1989) Changes in major components of japanese apricot during ripening. J. Korean Soc. Food Nutr., 18, 101-108
  23. Yun, S.I., Choi, W.J., Choi, Y.D., Lee, S.H., Yoo, S.H., Lee, E.H. and Ro, H.M. (2003) Distribution of heavy metals in soils of Shihwa tidal freshwater marshes. Korean J. Ecol., 26, 65-70 https://doi.org/10.5141/JEFB.2003.26.2.065
  24. Swain, T., Hillis, W.E. and Ortega, M. (1959) Phenolic constituents of Ptunus domestica I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric., 10, 83-88
  25. Nieva-Moreno, M.I., Isla, M.I., Sampietro, A.R. and Vattuone, M.A. (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol., 71, 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  26. Hwang, J.B., Yang, M.O. and Shin, H.K. (1997) Survey for approximate composition and mineral content of medicinal herbs. Korean J. Food Sci. Technol., 29, 671-679
  27. Kim, N.W., Joo, E.Y. and Kim, S.K. (2003) Analysis on the components of the fruit of Elaeagnus multiflora Thumb. Korean J. Food Preserv., 10, 534-539
  28. Joo, E.Y. and Kang, W.J. (2005) Analysis on the components of the Angelica dahurica root. Korean J. Food Preserv., 12, 476-481
  29. Lee, Y.S. (2007) Analysis of components of Angelica dahurica leaves. Korean J. Food Preserv., 14, 492-496
  30. Jeong, C.H., Joo, O.S. and Shin, K.H. (2002) Chemical components and physiological activities of young Mulberry (Morus alba) stem. Korean J. Food Preserv., 9, 228-233
  31. Lee, Y.S., Joo, E.Y. and Kim, N.W. (2005) Analysis on the components in stem of the Lespedeza bicolor. J. Korean Soc. Food Sci. Nutr., 34, 1246-1250 https://doi.org/10.3746/jkfn.2005.34.8.1246
  32. Choi, Y.W. (2002) Development of labor saving and environment friendly cultivation method for the production of high quality perilla leaf in Muryang area. The ministry of agriculture and forestry. The final research paper. p.322-339
  33. Solms, J. (1969) The taste of amino acids, peptides, and proteins. J. Agric. Food Chem., 17, 686-688 https://doi.org/10.1021/jf60164a016
  34. Mau, J.L., Chyau, C.C., Li, J.Y. and Tseng, Y.H. (1997) Flavor components in straw mushrooms Volariella volvacea harvested at different stages of maturity. J. Agric. Food Chem., 45, 4726-4729 https://doi.org/10.1021/jf9703314
  35. Chang, L., Xu, J.X., Zhao, J., Pang, Y.Z., Tang, C.S. and Qi, Y.F. (2004) Taurine antagonized oxidative stress injury induced by induced by homocysteine in rat vascular smooth muscle cells. ACTA Pharmarcol. Simica, 25, 341-346
  36. Chen, W., Matuda, K., Nishimura, N. and Yokogoshi, H. (2004). The effect of taurine on cholesterol degradation in mice fed a high-cholesterol diet. Life Sci., 74, 1889-1898 https://doi.org/10.1016/j.lfs.2003.08.041
  37. Huxtable, R.J. (1992) Physiological actions of taurine. Physiol. Rev., 72, 101-163 https://doi.org/10.1152/physrev.1992.72.1.101
  38. Kulakowaski, E.C. and Matsuo, J. (1984) Hypoglycemic properties if taurine nor mediated be enhanced insuline release. Biochem. Pharmacol., 33, 2835-2838 https://doi.org/10.1016/0006-2952(84)90204-1
  39. Kim, H.J., Kim, S.W. and Shin, C.S. (2000) Analysis of chemical composition in leaf and root of Acrorus calamus L. Korean J. Food Sci. Technol., 32, 37-41
  40. Lee, J.H. and Lee, S.R. (1994) Analysis of phenolic substances content of Korea plant foods. Korean J. Food Sci. Technol., 26, 310-316
  41. Sa, J.H., Jin, Y.S. Shin, I.C. Shim, T.H. and Wang, M.H. (2004) Photoprotective effect and antioxidative activity from different organs of Morus Bombycis Koidzumi. Kor. J. Pharmacogn., 35, 207-214
  42. Kim, E.Y., Baik, I.H., Kim, J.H., Kim, S.R. and Rhyu, M.R. (2004) Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol., 36, 333-338
  43. Lee, S.O., Lee, H.J., Yu, M.H., Im, H.G. and Lee, I.S. (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol., 37, 233-240