Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effects of Dietary Mulberry Leaf on Loperamide-Induced Constipation in Rats

식이뽕잎이 흰쥐의 Loperamide로 유도된 변비에 미치는 영향

  • Published : 2008.04.28
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Abstract

We investigated the effect of dietary mulberry leaf powder (MP) on loperamide-induced constipation in rats. Male Sprague-Dawley rats were given MP in their diets at a concentration of 0% 5% and 10% for 33 days. Rats were divided into 4 groups: normal diet group (NOR), normal diet and loperamide treated group (MPL0), 5% MP and loperamide treated group (MPL5), and 10% MP and loperamide treated group (MPL10). Constipation was induced by subcutaneous injection of loperamide (1.5 mg/kg body weight/day) for the final 5 days of the experiment Supplemental MP had no effect on the food efficiency ratio, but it reduced body weight gain and food intake in a concentration dependent manner. Administration of loperamide decreased food intake. MP had a concentration-dependent effect on decreasing total cholesterol, LDL-cholesterol, and triglycerides and on increasing HDL-cholesterol. Loperamide had no significant effect on serum lipid profiles. Loperamide decreased the number and wet weight of fecal pellets and fecal water content MP increased the number and wet weight of fecal pellets and fecal water content in a dose-dependent manner. In addition, MP increased gut transit time and transit speed, and the guts of mts treated with MP plus loperamide were longer than those of mts treated with loperamide alone. These results indicate that MP is an effective treatment for constipation.

뽕잎분말을 식이에 각각 0%(무첨가), 5%(저농도) 및 10%(고농도)로 33일간 흰쥐에게 급여하고, 실험 29-33일 사이에 5일간 1일 2회 loperamide(1.5 mg/kg/day, s.c.)를 피하투여하여 변비를 유도한 뒤 뽕잎분말의 변비 개선효과를 측정하였다. 체중증가량은 loperamide 투여 전에는 뽕잎분말을 급여한 MPL5군과 MPL10군이 뽕잎분말을 첨가하지 않고 정상식이를 급여한 NOR군과 MPL0군에 비하여 감소하였으며, 뽕잎분말 고용량을 급여한 MPL10군에서만 유의차를 보였다. 변비 유발 기간중에는 처리군 간의 유의차가 없었다. 식이섭취량은 변비유발 기간 중 loperamide만을 단독 투여한 MPL0군이 NOR군에 비하여 유의하게 저하되었다. 대장관내 변의 개수는 loperamide 단독 처리군(MPL0)에 비하여 뽕잎분말 병용 처리군(MPL5와 MPL10)이 용량 의존적으로 감소하였으며, 뽕잎분말 고용량을 처리한 MPL10군은 NOR군과 유사하였다. 장 통과시간과 통과속도는 loperamide 단독 처리군(MPL0)에 비하여 뽕잎분말을 병용 처리한 모든 군(MPL5와 MPL10)에서 감소되었다. 장의 길이는 뽕잎분말 처리군(MPL5와 MPL10)들이 정상대조군(NOR)이나 loperamide 단독 처리군(MPL0)에 비하여 유의하게 길었으며, 뽕잎분말의 첨가 수준이 증가할수록 장의 길이가 길었다. Loperamide 만을 단독으로 처리한 군(MPL0)은 변의 개수, 변의 중량 및 변의 수분 함량이 모두 유의적으로 저하되었다. Loperamide와 뽕잎분말을 병용 처리한 군(MPL5와 MPL10)들은 loperamide 단독 처리군 (MPL0)에 비하여 변의 개수, 변의 중량 및 변의 수분 함량이 유의하게 증가하였으며, 정상대조군(NOR)과 비슷한 수치를 보였다. 또한 뽕잎분말을 급여한 군(MPL5와 MPL10)들은 혈청 중 총콜레스테롤 및 LDL-콜레스테롤 함량이 정상식이를 급여한 군(NOR와 MPL0)들에 비하여 낮은 경향이었으며, HDL-콜레스테롤 함량은 높은 경향이었다. Loperamide 투여는 혈청 지질 농도 변화에는 영향을 미치지 않았다. 이상의 결과 뽕잎분말은 실험동물에서 loperamide 처치에 의해서 감소되어진 변의 개수, 변의 중량 및 변의 수분 함량을 증가시키며, 장내 변의 개수, 장 통과속도와 통과시간을 감소시켜 변비증상을 개선하는 효과를 보였다.

Keywords

References

  1. Corazziari, E. (1999) Need of the drug for the treatment of chronic constipation. Ital. J. Gastroenterol. Hapatol., 31, 232-233
  2. Corfield, A.P., Carroll, D., Myerscouh, N. and Probert, C.S.J. (2001) Mucins in the gastrointestinal tract in health and disease. Front. Biosci., 6, 1321-1357 https://doi.org/10.2741/Corfield
  3. Li, S.Z. (1978) Compendium of materia medica. People's Medical Publishing House, Beijing, p.2067
  4. Katai, K. (1942) Trace components in mulberry leaves. J. Chem. Soc. Jpn., 18, 379-383
  5. Kondo, Y. (1957) Trace constituents of mulberry leaves. Nippon Sanshigaku Zasshi, 26, 349-352
  6. Chae, J.Y., Lee, J.Y., Hoang, I.S., Whangbo, D., Choi, P.W., Lee, W.C., Kim, J.W., Kim, S.Y., Choi, S.W. and Rhee, S.J. (2003) Analysis of functional components of leaves of different mulberry cultivars. J. Korean Soc. Food Sci. Nutr., 32, 15-21 https://doi.org/10.3746/jkfn.2003.32.1.015
  7. Markham, K.R. (1989) Flavones, flavonols and their glycosides. Methods Plant Biochem., 1, 197-235
  8. Bang, H.S., Lee, W.C., Shon, H.R., Choi, Y.C. and Kim, H.B. (1998) Varietal comparison of $\gamma$-aminobutyric acid content in mulberry root bark. Kor. J. Seric. Sci., 40, 13-16
  9. Kim, S.Y., Ryu, K.S., Lee, W.C., Ku, Ho., Lee, H.S. and Lee, K.R. (1999) Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice. Kor. J. Pharmacogn., 30, 123-129
  10. Yoshikumi, Y. (1994) Inhibition of intestinal $\alpha$-glucosidase activity and postprandial hypoglycemia by moranoline and its N-alkyl derivatives. Agric. Biol. Chem., 52, 121-126
  11. Asano, N., Oseki, K., Tomioka, E., Kizu, H. and Matsui, K. (1994) N-containing sugars from Morus alba and their glucosidase inhibitory activities. Carbohydr. Res., 259, 243-255 https://doi.org/10.1016/0008-6215(94)84060-1
  12. Kim, S.Y., Lee, W.C., Kim, H.B., Kim, A.J. and Kim. S.K. (1998) Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia rats. J. Korean Soc. Food Sci. Nutr., 27, 1217-1222
  13. Lee, H.S., Kim, S.Y., Lee W.C., Lee, S.D., Moon, J.Y. and Ryu K.S. (2000) Effects of dietary mulberry leaf powder on gastrointestinal function of rat. J. Korean Soc. Food Sci. Nutr., 29, 865-869
  14. Lee, H.J. and Hwang, E.H. (1997) Effects of alginic acid, cellulose and pectin level on bowel function in rats. Kor. J. Nutr., 30, 456-477
  15. Burkitt, D.P., Waker, A.R.P. and Painter, N.S. (1974) Dietary fiber and disease. J. Am. Med. Assoc., 229, 1068-1074 https://doi.org/10.1001/jama.229.8.1068
  16. Gordon, D.T. (1992) The importance of total dietary fiber in human nutrition and health. Kor. J. Nutr., 25, 75-76
  17. Gordon, D.T. (1989) Functional properties vs physiological action of total dietary fiber. Cereal Foods World, 34, 517-525
  18. Chung, M.G., Song, C.W., Moon, J.S., Jeen, Y.T., Um, S.H., Kim, C.D. and Ryu H.S. (1996) Effects of psyllium husk on slow transit constipation. Kor. J. Gastroenterol., 28, 513-519
  19. Cumming, J.H., Bingham, S.A., Heaton, K.W. and Eastwood, M.A. (1992) Fecal weight, colon cancer risk and dietary intake of nonstarch polysaccharides (dietary fiber). Gastroenterol., 102, 1783-1789
  20. Reeves, P.G., Nielson, F.H. and Fahey Jr, G.C. (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr., 123, 1939-1951 https://doi.org/10.1093/jn/123.11.1939
  21. Shimotoyodome, A., Meguro, S., Hase, T., Tokimitsu, I. and Sakata, T. (2000) Decreased colonic mucus in rats with loperamide-induced constipation. Comp. Biochem. Physiol., 126, 203-211 https://doi.org/10.1016/S1095-6433(00)00194-X
  22. Heanton, J.M., Lennard-Jones, J.E. and Young, A.C. (1969) A new method for studying gut transit times using radiopaque marker. Gut, 10, 842-847 https://doi.org/10.1136/gut.10.10.842
  23. Friedwald, W.T., Levy, R.L. and Fredrickson, D.S. (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem., 18, 499-502
  24. Gabel, K., Newman, R., Lauritzen, G., Auld, G., Bock, M., Beuhn, C., Lee, Y., Medeiros, D., McNulty, J. and Nitzke, S. (1997) Fat and fiber knowledge and behaviors related to body mass index. Nutr. Res., 17, 1643-1753 https://doi.org/10.1016/S0271-5317(97)00171-1
  25. Blaak, E.E. and Saris, W.H.M. (1995) Health aspects of various digestible carbohydrates. Nutr. Res., 15, 1547-1573 https://doi.org/10.1016/0271-5317(95)02027-S
  26. Jeon, J.R., Kim, J.Y. and Choi, J.H. (2007) Effect of yam yogurt on colon mucosal tissue of rats with loperamide-induced constipation. Food Sci. Biotechnol., 16, 605-609
  27. Lumpton, J.R. and Morin, J.L. (1993) Barly bran flour accelerates gastrointestinal transit times. J. Am. Diet Assoc., 93, 881-885 https://doi.org/10.1016/0002-8223(93)91526-V
  28. McDougall, G.J., Morrison, I.M. and Hillman, J.R. (1996) Plant cell walls dietary fiber: range, structure, processing and function. J. Sci. Food Agric., 70, 133-150 https://doi.org/10.1002/(SICI)1097-0010(199602)70:2<133::AID-JSFA495>3.0.CO;2-4
  29. Spiller, G.A., Cheronoff, M.C., Hill, R.A., Gates, J.E., Nassar, J.J. and Shiply, E,A. Effect of purified cellulose, pectin and a low residue diet on fecal volatile fatty acids, transit time and fecal weight in humans. Am. J. Clin. Nutr., 33, 754-760
  30. Park, Y.K. and Kang, Y.H. 2004. Effects of single cells of carrot and radish on the fecal excretion properties, mineral absorption rate and structure of small intestine and colon in rats. J. Korean Soc. Food Sci. Nutr., 33, 505-511 https://doi.org/10.3746/jkfn.2004.33.3.505
  31. Kim, M.J. (1994) Effect of dietary fiber on the serum lipid level and bowel function in rats. M.S. Thesis. Hanyang University
  32. Judd, P.A. and Truswell, A.S. (1985) The hypocholesterolemic effects of pectins in rats. Br. J. Nutr., 53, 409-424 https://doi.org/10.1079/BJN19850051
  33. Yook, H.S., Kim, Y.H., Ahn, H.J., Kim, D.H., Kim, J.O. and Byun, M.W. (2000) Reological properties of wheat flour dough and qualities of bread prepared with dietary fiber purified from ascidian (Halocynthia roretzi) tunic. Korean J. Food Sci. Technol., 32, 387-395
  34. Yook, H.S., Kim, J.O., Choi, J.M., Kim, D.H., Cho, S.K. and Byun, M.Y. (2003) Changes of nutritional characteristics and serum cholesterol in rats by the intake of dietary fiber isolated from asidian (Halocynthia roretzi) tunic. J. Korean Soc. Food Sci. Nutr., 32, 474-478 https://doi.org/10.3746/jkfn.2003.32.3.474
  35. Shimotoyodome, A., Yajima, N., Suzuki, J. and Tokimitsu, I. 2005. Effects of coingestion of different fibers on fecal excretion and cecal fermentation in rats. Nutr. Res., 25, 1085-1096 https://doi.org/10.1016/j.nutres.2005.09.018
  36. Schiller, L.R., Santa Ana, C.A., Moravski, S.C. and Fordtran, J.S. (1984) Mechanism of the antidiarrheal effect of loperamide. Gastroenterology, 86, 1475-1480
  37. Theoduro, V., Fioramonti, J., Hachet, T. and Bueno, L. (1991) Absorptive and motor components of the antidiarrheal action of loperamide: an in vivo study in pig. Gut., 11, 1355-1359
  38. Read, N.W. (1983) Speculations on the role of motility in the pathogenesis and treatment of diarrhea. J. Gastroenterol. Suppl., 84, 45-63
  39. Kim, A.J., Kim, S.Y., Choi, M.K., Kim, M.H., Han, M.R. and Chung, K.S. (2005) Effects of mulberry leaves powder on lipid metabolism in high cholesterol-fed rats. Korean J. Food Sci. Technol., 37, 636-641
  40. Russell, D.W. and Setchell, K.D.R. (1992) Bile acid biosynthesis. Biochem., 31, 4737-4749 https://doi.org/10.1021/bi00135a001