Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Anticancer Effect of Ascorbic Acid and Saengshik on CT-26 Colon Cancer

CT-26 결장암에 대한 비타민 C와 생식의 항암효과

  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Deung, Young-Kun (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Qi, Xu Feng (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Lee, Young-Mi (Biotechnology Research Institute, HDr Co., Ltd.) ;
  • Yoon, Yang-Suk (Biotechnology Research Institute, HDr Co., Ltd.) ;
  • Kim, Kwang-Yong (Instittue of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 최주봉 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 이영미 (에이취디알 기술연구소) ;
  • 윤양숙 (에이취디알 기술연구소) ;
  • 김광용 (연세대학교 원주의과대학, 기초의학연구소) ;
  • 장병수 (한서대학교 피부미용학과) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실)
  • Published : 2008.03.31
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Abstract

Uncooked powered diet (Saengshik) composed of grains, vegetables, mushrooms and fruits have various physiological functions including strong antioxidant and potent anticancer effects by many kinds of bioactive phytochemicals. The objective of present study was to identify the anticancer effects of vitamin C and saengshik on colon cancer induced by CT-26 cell line in BALB/c mice. As the result, the tumor volumes of vitamin C-mixed diet group (VC) showed no significant differences compared with control group (C) after subcutaneous injection of CT-26 cell lines. However saengshik group (S) showed a significant effect, inhibiting the growth of cancer by 56.2% ($4.8{\pm}9.0\;mm^3$), 48.1% ($80.8{\pm}60.0\;mm^3$), 43.2% ($135.2{\pm}117.2\;mm^3$), 55.5% ($233.6{\pm}248.2\;mm^3$), 69.2% ($304.6{\pm}442.5\;mm^3$) and 70.7% ($464.9{\pm}705.9\;mm^3$) respectively as compared with C group at an interval of 5 days after injection of the CT-26 cells into mice. Also the final tumor volume of S group exerted a significant differences as compared with one of C group (p<0.05). Especially in the case of S group (n=10), the tumors in 2 of 10 mice entirely disappeared at 25th day. Our results suggest that saengshik possess a strong inhibitory action against tumor growth induced by CT-26 colon cancer cell line in the mice. Further studies of saengshik are required to confirm the cancer prevention effect and possibility of adjuvant cancer therapy.

곡류, 두류, 과채류, 해조류, 버섯류 등의 식물성 원료를 가열처리하지 않은 상태에서 분말화한 것을 혼합한 생식의 성분 중 생리활성물질은 강력한 항산화 기능 및 일부 항암효과를 포함한 생리활성기능을 나타낸다. 본 실험은 BALB/c 마우스에 CT-28 대장암세포를 피하주사한 후 비타민 C와 생식을 급이함으로써 암조직의 증식에 따른 항암효과를 확인하기 위하여 수행하였다. 각 실험군은 CT-26 세포를 피하주사한 후 암조직의 크기를 5일 간격으로 6회 측정한 결과 비타민 C를 급이한 군은 암을 유도한 군과 암조직의 부피를 비교한 결과 통계학적으로 유의성은 없었으나 암세포 주입 후 10, 15, 20일째의 평균 암조직 부피에서 대조군은 각각 $155.8{\pm}75.8\;mm^3$, $238{\pm}102.9\;mm^3$, $525.0{\pm}291.7\;mm^3$인데 비해서 생식군은 48.1% ($80.8{\pm}60.0\;mm^3$), 43.2% ($135.2{\pm}117.2\;mm^3$), 55.5% ($233.6{\pm}248.2\;mm^3$)의 억제율을 보여주었다. 특히 25일과 30일째에는 대조군의 암조직 부피가 $989.7{\pm}596.8\;mm^3$와 $1589.7{\pm}1071.0\;mm^3$로 급격히 증가한 반면 생식군은 $304.6{\pm}442.5\;mm^3$와 $464.9{\pm}705.9\;mm^3$로 대조군에 비해 각각 69.2%와 70.7%의 감소율을 보임으로써 암조직의 증식이 현저히 억제된 결과를 보여주었다. 특히 30일째 암조직의 차이는 통계학적으로도 유의한 차이를 나타내었다(p<0.05). 따라서 생식은 CT-26 결장암 세포주에 의해 유도된 암증식을 억제하는 효과가 있는 것으로 생각된다.

Keywords

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