Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Free Radical Scavenging Activity of Methanol Extracts of Chungkukjang

  • Seo, Kyoung-Chun (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) ;
  • Noh, Jeong-Sook (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) ;
  • Yi, Na-Ri (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) ;
  • Choi, Ji-Myung (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) ;
  • Cho, Eun-Ju (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) ;
  • Han, Ji-Sook (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University) ;
  • Song, Yeong-Ok (Department of Food Science and Nutrition and Kimchi Research Institute, Pusan National University)
  • Published : 2008.06.30
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Abstract

To further the goal of isolating Bacillus sp. from commercial chungkukjang (CKJ) for a development of a probiotic dietary adjunct using soymilk or milk, antioxidant activity of CKJ purchased from the Sunchang Traditional Village in Chunbook province was examined. Six CKJ samples were evaluated and 3 were selected based on the results of the physicochemical analysis and sensory evaluation for further antioxidant study. $IC_{50}$ for DPPH scavenging activity of methanol extracts of CKJ ranged from 238.1 to 345.7 ${\mu}g/mL$. CKJ exhibited over 80% scavenging of ${\cdot}OH$ and ONOO- at concentrations of 100 ${\mu}g/mL$ and 250 ${\mu}g/mL$, respectively. $O_2^-$ and NO scavenging activities of three CKJ were increased in a dose dependent manner with the concentration tested from 100 to 1000 ${\mu}g/mL$. In this study, the methanol extract of CKJ exhibited a great reduction capability and powerful free radical scavenging activity, especially against OH. and ONOO-, which are the most toxic radicals responsible for oxidative damage in the body. However, radical scavenging effects of CKJ on DPPH, $O_2^-$, and nitrite radical were rather moderate. In conclusion, CKJ may reduce the oxidative stress in the body by scavenging the free radicals.

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References

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