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Antioxidative and Biological Activity of Hot Water and Ethanol Extracts from Phellinus linteus

상황버섯 열수 및 에탄올 추출물의 항산화 효과 및 생리활성

  • Published : 2008.06.30

Abstract

To investigate the applicability of hot water extract (PLW) and ethanol extract (PLE) from Phellinus linteus as functional food and cosmeceutical materials, its total flavonoids content, total phenolics content, electron donating ability (EDA), nitrite-scavenging ability (NSA), SOD-like activity, inhibitory effect of tyrosinase and elastase were examined. Total flavonoids contents of PLW and PLE were 17.31 mg/g and 42.61 mg/g, respectively, and total phenolics contents were estimated as 149.92 mg/g for PLW and 432.42 mg/g for PLE. The EDA of PLW and PLE were $6.49{\sim}92.98%$ and $22.61{\sim}94.28%$. The EDA and total phenolics contents had a high correlation of 0.83. The NSA was pH dependent, and was highest at pH 1.2 and lowest at pH 6.0. The NSA of PLE was higher than that of PLW. The SOD-like activities of PLW and PLE were $14.36{\sim}35.21%$ and $17.27{\sim}81.84%$, respectively, and the activity was dependent on the sample concentration. The tyrosinase inhibitory activity was the highest in PLE ($10.51{\sim}80.93%$) while that of PLW was $4.77{\sim}43.69%$. Finally, the elastase inhibitory activity was $10.01{\sim}76.02%$ at PLE. Based on the above results, we deemed that the ethanol extract of Phellinus linteus was the most pertinent for use as functional food and cosmeceutical materials.

상황버섯의 기능성식품 및 화장품 소재로서의 이용 가능성을 조사하기 위해 상황버섯 열수 및 에탄올 추출물의 총플라보노이드 함량, 총페놀 화합물 함량, 전자공여능, 아질산염소거능, SOD 유사활성, tyrosinase의 저해활성 및 elastase 저해활성을 측정하였다. 상황버섯 추출물의 총플라보노이드 함량은 열수 추출물 17.31 mg/g, 에탄올 추출물 42.61 mg/g이었으며, 총페놀성 화합물은 열수 추출물 149.92 mg/g, 에탄올 추출물 432.42 mg/g으로 에탄올 추출물에서 총플라보노이드 및 총페놀성 화합물을 다량 함유한 것으로 나타났다. 전자공여능은 열수 추출물에서 $6.49{\sim}92.98%$, 에탄올 추출물에서 $22.61{\sim}94.28%$의 범위였으며, 에탄올 추출물에서 비교적 높은 활성을 나타내었다. 또한 총페놀성 화합물 함량과 전자공여능 사이에 높은 상관관계($R^2=0.83$)가 있는 것으로 나타났다. 아질산염소거능은 모든 pH 조건하에서 에탄올 추출물이 열수 추출물보다 높은 아질산염소거능을 나타내었으며, pH가 증가할수록 감소하였다. 상황버섯 추출물의 SOD 유사활성을 측정한 결과 열수 추출물에서는 $14.36{\sim}35.21%$, 에탄올 추출물에서는 $17.27{\sim}81.84%$의 범위로 분석되었으며, 농도에 따른 유의적 증가현상을 나타내었다. 상황버섯 추출물에 대하여 tyrosinase의 저해활성을 측정한 결과 열수 추출물에서 $4.77{\sim}43.69%$, 에탄올 추출물에서 $10.51{\sim}80.93%$의 저해활성을 나타내어, 에탄올 추출물에서 tyrosinase 저해활성이 우수함을 확인하였다. 또한 상황버섯의 피부주름 개선 물질로서의 사용 가능성을 조사한 결과 에탄올 추출물이 $10.01{\sim}76.02%$의 elastase 저해활성을 나타내었다.

Keywords

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