Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Antioxidant and free radical scavenging activities of Cleome rutidosperma

  • Published : 2008.06.30
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Abstract

The study was aimed at evaluating the antioxidant and free radical scavenging activities of ethanolic extract and its fractions of Cleome rutidosperma. The antioxidant activity, reducing power, total phenolic content, total flavonoid content, superoxide anion scavenging activity, nitric oxide anion scavenging activity, in vitro antilipid peroxidation activity and in vitro non-enzymatic hemoglobin glycosylation were studied. The results obtained in the study indicate that Cleome rutidosperma is a potential source of natural antioxidant. All the parameters were found to be concentration dependent and increased with increasing amounts of sample. Flavonoids, phenolic compound like tannins, terpenoids may be responsible for the antioxidant activity of the plant. Variation of solubility parameters in various models may be attributed to non-linearity of activity of ethanol extract fractions models. Further investigation on the isolation and identification of antioxidant component(s) in the plant may lead to chemical entities with potential for clinical use.

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References

  1. Beauchamp C, Fridovich I. (1971) Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276-277 https://doi.org/10.1016/0003-2697(71)90370-8
  2. Beheti JR, Kumar V, Shah GB, Goyal RK. (2005) Free radical scavenging activity of aqueous extract of Rhus succedanae galls. J. Nat. Rem. 5, 15-18
  3. Beninger CW, Hosfield GL. (2003) Antioxidant activity of extracts, condensed tannin fractions and pure flavonoids from Phaseolus vulgaris L. seed coat color genotypes. J. Agric. Food Chem. 51, 7879-7883 https://doi.org/10.1021/jf0304324
  4. Bidla G, Titanji VPK, Joko B, El-Ghazali G, Bolad A, Berzins K. (2004) Antiplasmodial activity of seven plants used in African folk medicine. Indian J. Pharmacol. 36, 245-246
  5. Bose A, Saravanan VS, Karunanidhi N, Gupta JK. (2004) Analgesic and Locomotor Activity of Extracts of Cleome rutidosperma DC. Indian J. Pharm. Sci. 66, 795-797
  6. Bose A, Gupta JK, Ghosh T, Dash GK. (2005) Antimicrobial activity of certain extracts of Cleome rutidosperma. Indian J. Nat. Prod. 21, 39-41
  7. Bose A, Mondal S, Gupta JK, Dash GK, Ghosh T, Si S. (2006) Studies on diuretic and laxative activity of ethanolic extract and its fractions of Cleome rutidosperma aerial parts. Pharmacognosy Mag. 2, 27-31
  8. Cakir A, Mavi A, Yildrim ME, Deru MH, Kazaz C. (2003) Isolation and characterization of antioxidant phenolic compounds from aerial parts of Hypericum hyssopifolium L. by activity guided fractionation. J. Ethnopharmacol. 87, 73-83 https://doi.org/10.1016/S0378-8741(03)00112-0
  9. Chang C, Yang M, Wen H, Chern J. (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10, 178-182
  10. Cook NC, Samman S. (1996) Flavonoids- chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Biochem. 7, 66-76 https://doi.org/10.1016/0955-2863(95)00168-9
  11. Cooks JA, Varderjag DJ, Dasgupta A, Mounkaila G, Glew RS, Blackwell W. (1998) Use of the trolox assay to estimate the antioxidant content of seventeen edible wild plants of niger. Life sci. 63, 105-110 https://doi.org/10.1016/S0024-3205(98)00245-8
  12. Das NP, Pereira TA. (1990) Effects of flavonoids on thermal autooxidation of Palm oil: structure- activity relationship. J. Am. Oil Chem. Soc. 67, 255-258 https://doi.org/10.1007/BF02540652
  13. Diplock AI. (1997) Will the 'good fairies' please proves to us that vitamin F lessens human degenarative of disease? Free Radic. Res. 27, 511-532 https://doi.org/10.3109/10715769709065791
  14. Fluckiger R, Winterhalter KH. (1978) Biochemical and Clinical Aspects of Hemoglobin Abnormalities, p. 208, Academic Press, New York
  15. Freeman BA, Crapo JD. (1982) Biology of Diseases: Free radicals and tissue injury. Lab. Invest. 47, 412-426
  16. Freeman BA, Crapo JD. (1988) The antioxidants of higher plants. Phytochemistry 27, 969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  17. Formica JV, Regelson W. (1995) Review of the biology of quercetin and related biflavonoids. Food Chem. Toxicol. 33, 1061-1080 https://doi.org/10.1016/0278-6915(95)00077-1
  18. Halliwell B, Gutteridge JMC. (1985) In: Free radicals, ageing, and disease, Free radicals in Biology and Medicine. 2nd ed., Clarendron Press, Oxford, pp. 279-315
  19. Halliwell H. (1994) Free radicals, antioxidants, and human disease: Curiosity, cause or consequence. Lancet 344, 721-724 https://doi.org/10.1016/S0140-6736(94)92211-X
  20. Hatano T, Edamatsu R, Mori A, Fujita Y, Yasuhara E. (1989) Effect of tannins with co-existing substances: Effect of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chem. Pharm. Bull. 37, 2016-2021
  21. Heinoman M, Rein D, Satue-Gracia MT, Huang SW, German JB, Frankel EN. (1998) Effect of protein on the antioxidant activity of phenolic compounds in a lecithin-liposome oxidation system. J. Agric. Food Chem. 46, 917-922 https://doi.org/10.1021/jf970826t
  22. Kessler M, Ubeaud G, Jung L. (2003) Anti- and prooxidant activity of rutin and quercetin derivatives. J. Pharm. Pharmacol. 55, 131-142 https://doi.org/10.1211/002235702559
  23. Kiritikar KR, Basu BD. (1991) Indian Medicinal Plants, p. 181 Lalit Mohan Basu, Deharadun, India
  24. Kumar MS, Sadique J. (1987) The biochemical mode of action of Gynandropsis gynandra in inflammation. Fitoterapia 58, 379-385
  25. Larson, RA. (1988) The antioxidants of higher plants. Phytochemistry 27, 969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  26. Malencic D, Gasic O, Popovic M, Boza P. (2000) Screening for antioxidant properties of Salvia reflexa hornem. Phytother. Res. 14, 546-548 https://doi.org/10.1002/1099-1573(200011)14:7<546::AID-PTR658>3.0.CO;2-K
  27. Maxwell SR, Lip GY. (1997) Free radicals and antioxidants in cardiovascular disease. Br. J. Clin. Pharmacol. 44, 307-317 https://doi.org/10.1046/j.1365-2125.1997.t01-1-00594.x
  28. Meir S, Kanner J, Akiri B, Hadas SP. (1995) Determination and involvement of aqueous reducing compounds in oxidative defence systems of various senesing leaves. J. Agric. Food Chem. 43, 1813-1817 https://doi.org/10.1021/jf00055a012
  29. Miyake T, Shibamoto T. (1997) Antioxidant activities of natural compounds found in plants. J. Agric. Food Chem. 45, 1819-1822 https://doi.org/10.1021/jf960620c
  30. Moncada A, Palmer RMJ, Higgs EA. (1991) Nitric oxide: Physiology, Pathophysiology and Pharmacology. Pharmacol. Rev. 43, 109-142
  31. Montilla MP, Ahmad A, Navarro MC, Jimenez MI, Garcia-Granados A, Parra AM, Cabo M. (2003) Antioxidant Activity of Maslinic Acid, a Triterpene Derivative Obtained from Olea europaea. Planta Med. 69, 472-474 https://doi.org/10.1055/s-2003-39698
  32. Mukherjee PK. (2002) Pharmacological screening of herbal drugs. Quality Control of Herbal Drugs-An Approach to Evaluation of Botanicals. pp. 519-582, Business Horizons, New Delhi, India
  33. Harborne, JB. (1984) Phytochemical methods: A Guide to modern techniques of plant analysis, 2nd ed., p. 85, Chapman and Hall, New York
  34. Ohkawa H, Ohishi N, Yagi K. (1979) Assay for lipid peroxidation on animal tissue by thiobarbituric acid reaction. Anal. Biochem. 18, 909
  35. Oyaizu M. (1986) Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44, 307-315
  36. Prieto P, Pineda M, Aguilar M. (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E. Anal. Biochem. 269, 337-341 https://doi.org/10.1006/abio.1999.4019
  37. Pryor WA. (1973) Free radical reactions and their importance in bio-chemical systems. Fed. Proc. 32, 1862-1869
  38. Ravishankara MN, Shrivastava N, Padh H, Rajani M. (2002) Evaluation of antioxidant properties of root bark of Hemidesmus indicus R. Br. (Anantmul). Phytomedicine 9, 153-160 https://doi.org/10.1078/0944-7113-00104
  39. Sanchez-Moreno C, Larrauri JA, Saura-Calixto F. (1999) Free radical scavenging capacity an inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res. Intern. 32, 407-412 https://doi.org/10.1016/S0963-9969(99)00097-6
  40. Slinkard K, Singleton VL. (1977) Total phenol analyses automation and comparison with manual methods. Am. J. Enol. Vitic. 28, 49-55
  41. Stajner D, Milic N, Mimica-Dukic N, Lazic B, Igic R. (1998) Antioxidant abilities of cultivated and wild species of garlic. Phytother. Res. 12, 513-5141
  42. Sreejayan, Rao MN. (1997) Nitric oxide scavenging by curcuminoids. J. Pharm. Pharmacol. 49, 105-107
  43. Trease, GE, Evans WC. (1989) Pharmacognosy, 13th ed., p. 171, ELBS Publication, New Delhi, India
  44. Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP. (1996) Bacopa monniera Linn. As an antioxidant: Mechanism of action. Indian J. Exp. Biol. 34, 523-526
  45. Vani T, Rajani M, Sarkar S, Shishoo CJ. (1997) Antioxidant properties of the Ayurvedic formulation Triphala and its constituents. Int. J. Pharmacog. 35, 313-317
  46. Waterhouse B, Mitchell A. (1998) Northern Australia Quarantine Strategy Weeds Target List. p. 29, AQIS Miscellaneous Publication, Canberra
  47. Widespread JM. (1972) Capparidaceae. Flora Malesiana: Series I 6, 61
  48. Yadav SP, Tripathi V, Singh RK, Pandey HP. (2000) Antioxidant activity of Cuscuta reflexa Steam. Indian J. Pharm. Sci. 62, 477-478
  49. Yildirim A, Oktay M, Bilaloqlu V. (2001) Comparison of antioxidant and antimicrobial activities of Tilia, Sege and Black Tea extracts. Turkish J. Med. Sci. 31, 23-27
  50. Younes M. (1981) Inhibitory action of some flavonoids on enhanced spontaneous lipid peroxidation following glutathione epletion. Planta Med. 43, 240-245 https://doi.org/10.1055/s-2007-971503
  51. Zhu M, Chang O, Wong LK, Chong FS, Li RC. (1999) Triterpene antioxidants from Ganoderma lucidum. Phytotherapy Res. 13, 529-531 https://doi.org/10.1002/(SICI)1099-1573(199909)13:6<529::AID-PTR481>3.0.CO;2-X

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