Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Characterization of Protein Expression in the Head of Oryzias latipes in Response to Acute and Chronic Exposure to Benzo(a)pyrene

  • Oh, Jeong-Hwan (Department of Biological Sciences, Pusan National University) ;
  • Moon, Hyo-Bang (Marine Environment Research Team, National Fisheries Research and Development Institute) ;
  • Choe, Eun-Sang (Department of Biological Sciences, Pusan National University)
  • Published : 2008.08.31
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Abstract

In this study, alterations in whole proteome expression patterns in the head of Japanese medaka (Oryzias latipes) was investigated following acute or chronic exposure to benzo(a)pyrene (BaP) (25 ${\mu}g$ L$^{-1}$) for 48 hrs and 15 days, respectively. The results showed that 9 and 6 protein spots were statistically different, relative to controls, in response to acute and chronic BaP exposure, respectively. In the acute exposure group, 5 spots were up regulated and 4 spots were downregulated, while in the chronic exposure group, 4 spots were upregulated and 2 spots were downregulated. Three of these spots were common to both the acute and chronic BaP exposure groups and were identified using LC-MS/MS followed by database searching. These 3 spots were found to be associated with structural proteins belonging to the actin and keratin families. These data suggest that acute and chronic exposures to BaP may affect tissue morphology in the head of Japanese medaka.

Keywords

References

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