Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Analysis of Bacterial Community Structure of Biological Activated Carbon Process in Drinking Water Treatment Plant Using FISH

FISH법을 이용한 정수처리장 내 생물활성탄 공정의 세균군집 구조 분석

  • Son, Hyeng-Sik (Department of Microbiology, Pusan National University) ;
  • Kim, Mi-A (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Jeong, Seong-Yun (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Kim, Young-Hun (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Son, Hee-Jong (Water Quality Research Institute, Waterworks Headquarter) ;
  • Park, Geun-Tae (Research & University-Industry Cooperation, Pusan National University) ;
  • Kim, Min-Ju (Department of Microbiology, Pusan National University) ;
  • Ryu, Eun-Yeon (Department of Microbiology, Pusan National University) ;
  • Lee, Sang-Joon (Department of Microbiology, Pusan National University)
  • 손형식 (부산대학교 미생물학과) ;
  • 김미아 (부산대학교 Bio-IT 파운드리센터) ;
  • 정성윤 (부산대학교 Bio-IT 파운드리센터) ;
  • 김영훈 (부산대학교 Bio-IT 파운드리센터) ;
  • 손희종 (부산광역시 상수도사업본부) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 김민주 (부산대학교 미생물학과) ;
  • 유은연 (부산대학교 미생물학과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2008.05.30
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Abstract

The bacterial community structure in biological activated carbon (BAC) process in drinking water treatment plant was investigated by Fluorescent in situ Hybridization (FISH) with rRNA-targeted oligonucleotide probe. Samples were collected at different three points in BAC process every month for one year. They were hybridized with a probe specific for the alpha, beta, gamma subclass of the class Proteobacteria, Cytophaga-Flavobacteria group and Gram-positive high G+C content (HGC) group. Total numbers of bacteria in BAC process counted by 4',6-diamidino-2-phenylindole (DAPI) staining were $5.4{\times}10^{10}$ (top), $4.0{\times}10^{10}$ (middle) and $2.8{\times}10^{10}$ cells/ml (bottom). The number of the culturable bacteria was from $1.0{\times}10^7$ to $3.6{\times}10^7$ cells/ml and the culturability was about 0.05%. The faction of bacteria detectable by FISH with the probe EUB338 was about 83% of DAPI counts. Gamma and alpha subclass of the class Proteobacteria were predominant in BAC process and their ratios were over 20% respectively. In top and middle, alpha, beta and gamma subclass of the class Proteobacteria competed with each other and their percentages was changed according to the season. In bottom, gamma subclass of the class Proteobacteria was predominant all through the year. It could be successfully observed the seasonal distribution of bacterial community in biological activated carbon process using FISH.

Keywords

References

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