Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Heterologous Production and Detection of Recombinant Directing 2-Deoxystreptamine (DOS) in the Non-Aminoglycoside-Producing Host Streptomyces venezuelae YJ003

  • Kurumbang, Nagendra Prasad (Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University) ;
  • Oh, Tae-Jin (Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University) ;
  • Liou, Kwangkyoung (Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University) ;
  • Sohng, Jae-Kyung (Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University)
  • Published : 2008.05.31
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Abstract

2-Deoxystreptamine is a core aglycon that is vital to backbone formation in various aminoglycosides. This core structure can be modified to develop hybrid types of aminoglycoside antibiotics. We obtained three genes responsible for 2-deoxystreptamine production, neo7, neo6, and neo5, which encode 2-deoxy-scyllo-inosose synthase, L-glutamine: 2-deoxy-scyllo-inosose aminotransferase, and dehydrogenase, respectively, from the neomycin gene cluster. These genes were cloned into pIBR25, a Streptomyces expression vector, resulting in pNDOS. The recombinant pNDOS was transformed into a non-aminoglycoside-producing host, Streptomyces venezuelae YJ003, for heterologous expression. Based on comparisons of the retention time on LC-ESI/MS and ESI-MS data with those of the 2-deoxystreptamine standard, a compound produced by S. venezuelae YJ003/pNDOS was found to be 2-deoxystreptamine.

Keywords

References

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