Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibition of LPS induced iNOS, COX-2 and cytokines expression by kaempferol-3-O-${\beta}$-D-sophoroside through the $NF{-\kappa}B$ inactivation in RAW 264.7 cells

Kaempferol-3-O-${\beta}$-D-sophoroside의 RAW 264.7 세포에서 $NF{-\kappa}B$ 억제를 통한 LPS에 의해 유도되는 iNOS, COX-2 및 cytokine들의 발현 저해효과

  • Park, Seung-Jae (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Shin, Ji-Sun (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Cho, Woong (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Cho, Young-Wuk (Department of physiology, College of Medicine, Kyung-Hee University) ;
  • Ahn, Eun-Mi (Department of Herbal Food Science, Daegu Haany University) ;
  • Baek, Nam-In (Department of Life Science, Kyung-Hee University) ;
  • Lee, Kyung-Tae (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
  • Published : 2008.06.30
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Abstract

In the present study, we investigated the anti-inflammatory effects by kaempferol-3-O-${\beta}$-D-sophoroside (KS) isolated from Sophora japonica (Leguminosae) on the lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin ($PGE_2$) production by RAW 264.7 cell line compared with kaempferol. KS significantly inhibited the LPS-induced NO and $PGE_2$ production. Consistent with these observations, KS reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, the release and the mRNA expression levels of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-6 (IL-6) were also reduced by KS. Moreover, KS attenuated the LPS-induced activation of nuclear factor-kappa B ($NF{-\kappa}B$), a transcription factor necessary for pro-inflammatory mediators, iNOS, COX-2, $TNF-{\alpha}$ and IL-6 expression. These results suggest that the down regulation of iNOS, COX-2, $TNF-{\alpha}$, and IL-6 expression by KS are achieved by the downregulation of $NF{-\kappa}B$ activity, and that is also responsible for its anti-inflammatory effects.

Keywords

References

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