Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Betulinic Acid Isolated from Ziziphus jujuba Lam on Infection of Rotavirus in MA-104 Cell

대추나무 껍질 유래 Betulinic acid의 in vitro rotavirus 감염억제 효과

  • Published : 2008.06.30
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Abstract

This study was conducted to evaluate the inhibitory effects of betulinic acid isolated from Ziziphus jujuba on various human rotavirus, such as KU, S2 and YO. The results obtained are summarized as follows: At the concentration of betulinic acid 0.1/2%, all human rotavirus is showed a maximum effect on their growth even though it's evaluated on the in vitro test. The each inhibitory rate of MA-104 cells infected by human rotavirus KU, S2 and YO was 62.1%, 59.7% and 65.2%, respectively, at the concentration of 0.1/2%. The anti-virus activity of betulinic acid was showed as the dose-dependent manner at the used dosages except at the dose of 0.1%.

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References

  1. Pisha, E., Chai, H., Lee, I.S., Chagwedera, T.E., Farnsworth, N.R., Cordell, G.A., Beecher, C.W., Fong, H.H., Kinghorn, A.D., Brown, D.M., Wani, M.C., Wall, M.E., Hiekens, T.J., Das Gupta, T.K. and Pezzuto, J.M. (1995) Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis. Nat. Med. 1: 1046-1051 https://doi.org/10.1038/nm1095-1046
  2. Schmidt, M. L., Kuzmanoff, K.L., Ling-Indeck, L. and Pezzuto, J.M. (1997). Betulinic acid induces apoptosis in human neuroblastoma cell lines. Eur. J. Cancer 33: 2007-2010 https://doi.org/10.1016/S0959-8049(97)00294-3
  3. Fulda, S., Friesen, C., Los M., Scaffidi C., Mier W., Benedict, M., Nunez, G., Krammer, P.H., Peter, M.E. and Debatin K.M. (1997) Betulinic acid triggers CD95 (APO-1/Fas)- and p53- independent apoptosis via activation of caspases in neuroectodermal tumors. Cancer Res. 57: 4956-4964
  4. Jeong, H.J., Chai, H.B., Park, S.Y., Kim D.S. (1999) Preparation of amino acid conjugates of betulinic acid with activity against human melanoma. Bioorg. Med. Chem. Lett. 9:1201-1204 https://doi.org/10.1016/S0960-894X(99)00165-1
  5. Fujioka, T., Kashiwada, Y., Kilkuskie, R.E., Cosentino, L.M., Ballas, L.M., Jiang, J.B., Janzen, W.P., Chen, I.S. and Lee, K.H.(1994) Anti-AIDS agents II, Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum and the anti-HIV activity of structurally related triterpenoids. J. Nat. Prod. 57:243-247 https://doi.org/10.1021/np50104a008
  6. Kashiwada, Y., Hashimoto, F., Cosentino, L.M., Chen, C.H., Garrett, P.E. and Lee, K.H. (1996) Betulinic acid and dihydrobetulinic acid derivatives as potent anti-HIV agents. J. Med. Chem. 39:1016-1017 https://doi.org/10.1021/jm950922q
  7. Hashimoto, F., Kashiwada, Y., Cosentino, L.M., Chen, C.H., Garrentt, P.E. and Lee, K.H. (1997) Anti-AIDS agents- XXVII. Synthesis and anti-HIV activity of betulinic acid and dihydrobetulinic acid derivatives. Bioorg Med. Chem. 5:2133-2143 https://doi.org/10.1016/S0968-0896(97)00158-2
  8. Hess, S.C., Brum, R.L., Honda, N.K., Cruz, A.B., Moretto, E., Cruz, R.B., Messana, I., Ferrari, F., Cechinel Filho, V. and Yunes, R.A. (1995) Antibacterial activity and phytochemical analysis of Vochysia divergens (Vochysiaceae). J. Ethnopharmacol. 47:97-100 https://doi.org/10.1016/0378-8741(95)01260-K
  9. Feng, N., Burns, J.W., Bracy, L. and Greenberg, H.B. (1994) Comparison of mucosal and systemic humoral immune responses and subsequent protection in mice orally inoculated with a homologous or a heterologous rotavirus. J. Virol. 68:7766-7773
  10. Chiarini, A., Arista, S., Giammanco, A. and Sinatra, A. (1983) Rotavirus persistence in cell cultures: selection of resistant cells in the presence of foetal calf serum. J. Gen. Virol. 64:1101-10 https://doi.org/10.1099/0022-1317-64-5-1101
  11. Ruggeri, F.M. and Greenberg, H.B. (1991) Antibodies to the trypsin cleavage peptide VP8 neutralize rotavirus by inhibiting binding of virions to target cells in culture. J. Virol. 65: 2211-2219
  12. Weber, J.M., Ruzindana-Umunyana, A., Imbeault, L. and Sircar, S. (2003) Inhibition of adenovirus infection and adenain by green tea catechins. Antiviral Res. 58:167-173 https://doi.org/10.1016/S0166-3542(02)00212-7
  13. Kojima, H., Tominaga, H., Sato, S. and Ogura, H. (1987) Pentacyclic triterpenoids from Prunella vulgaris. Phytochemistry 26:1107-1111 https://doi.org/10.1016/S0031-9422(00)82359-6
  14. Mahato, S.B. and Kundu, A.P. (1994) $^{13}C$ NMR Spectra of pentacyclic triterpenoids - a compilation and some salient features. Phytochemistry 37:1517-1575 https://doi.org/10.1016/S0031-9422(00)89569-2
  15. Choi, S.Z., Yang, M.C., Choi, S.U. and Lee, K.R. (2006) Cytotoxic terpenes and lignans from the roots of Ainsliaea acerifolia. Arch. Pharm. Res. 29:203-208 https://doi.org/10.1007/BF02969394
  16. Bishop, R.F., Davidson, G.P., Holmes, I.H. and Ruck, B.J. (1973) Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet 2:1281-1283
  17. Kapikain, A.Z. and Chanock, R.M. (1991) Rotaviruses. In Fields, B.N.(ed), Virology, 1353-1404, Raven Press, New York
  18. Gouvea, V., de Castro, L., Timenetsky, M.C., Greenberg, H. and Santos, N. (1994) Rotavirus serotype G5 associated with diarrhea in Brazilian children. J. Clin. Microbiol. 32:1408-1409
  19. Kaga, E., Iizuka, M., Nakagomi, T. and Nakagomi, O. (1994) The distribution of G (VP7) and P (VP4) serotypes among human rotaviruses recovered from Japanese children with diarrhea. Microbiol. Immunol. 38:317-320 https://doi.org/10.1111/j.1348-0421.1994.tb01784.x
  20. Lanata, C.F. and Franco, M. (2006) Nitazoxanide for rotavirus diarrhoea? Lancet 368:100-101 https://doi.org/10.1016/S0140-6736(06)68981-2
  21. Ginn, D.I., Ward, R.L., Hamparian, V.V. and Hughes, J.H. (1992) Inhibition of rotavirus in vitro transcription by optimal concentrations of monoclonal antibodies specific for rotavirus VP6. J. Gen. Virol. 73:3017-3022 https://doi.org/10.1099/0022-1317-73-11-3017