Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Fumonisin B1 Induces Apoptosis in Sphingosine 1-Phosphate Lyase-null F9 Cells through Increase of Sphingolipids Levels

  • Pak, Seon-Mi (College of Pharmacy, Chungbuk National University) ;
  • Park, Nam-Young (College of Pharmacy, Chungbuk National University) ;
  • Park, Myung-Yong (College of Pharmacy, Chungbuk National University) ;
  • Kim, Wan-Jong (Department of Life Sciences, Soonchunhyang University) ;
  • Lee, Jong-Hwa (Department of Life Sciences, Soonchunhyang University) ;
  • Oh, Sei-Kwan (Department of Neuroscience, School of Medicine, Ewha Womans University) ;
  • Yoo, Hwan-Soo (College of Pharmacy, Chungbuk National University) ;
  • Lee, Yong-Moon (College of Pharmacy, Chungbuk National University)
  • Published : 2008.06.30
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Abstract

Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.

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References

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