Karyotypic Analysis and Physical Mapping of rRNA Gene Loci in Persicaria tinctoria

쪽의 핵형분석과 rRNA 유전자의 염색체상 위치

  • Choi, Hae-Woon (School of Biosci. & Biotech., Chungnam Natl. Univ.) ;
  • Lee, Sang-Hoon (School of Biosci. & Biotech., Chungnam Natl. Univ.) ;
  • Kim, Soo-Young (Korea Research Institute of Bioscience and Biotechnolgy (KRIBB)) ;
  • Bang, Jae-Wook (School of Biosci. & Biotech., Chungnam Natl. Univ.)
  • 최혜운 (충남대학교 생명과학부 생물학과) ;
  • 이상훈 (충남대학교 생명과학부 생물학과) ;
  • 김수영 (한국생명공학연구원) ;
  • 방재욱 (충남대학교 생명과학부 생물학과)
  • Published : 2008.06.30


Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.


  1. Bae KH (2000) The Medicinal Plants of Korea. Kyohaksa Publishing Co., Seoul., p. 85
  2. Choi HW, Koo DH, Bang KH, In DS, Kim YC, Seong NS, Bang JW (2007) Molecular cytogenetics in Panax ginseng and its related species. 16th Int. Chromosome Confer. p. 32
  3. Chung ES, Chae YA (1994) Factors affecting cell growth in suspension cultures of Polygonum tinctorium Lour. Korean J. Breed. 26:172-176
  4. Darlington CD, Wylie AP (1955) Chromosome Atlas of Flowering Plants. The McMillan Co., New York., p. 73
  5. Hong SP, Lee S (1983) A palynotaxonomic study of Korean Polygonaceae. Korean J. Plant Tax. 13:63-76 https://doi.org/10.11110/kjpt.1983.13.2.063
  6. Jiang J, Gill BS (2006) Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome 49:1057-1068 https://doi.org/10.1139/G06-076
  7. Kang SS, Woo WS (1982) A flavonoid glycocide from the leaves of Polygonum sachalinense (II). Arch. Pharm. Res. 5:13-15 https://doi.org/10.1007/BF02856358
  8. Kim SY, Choi HW, Bang JW (2004) Physical mapping of rDNAs using McFISH in Anemarrhena asphodeloides Bunge. Korean J. Medicinal Crop Sci. 12:515-518
  9. Kim SY, Bang, JW, Lee JK (2006a) Cytogenetic analyses using mitosis, meiosis chromosomes and bicolor fluorescence in situ hybridization of Bupleurum latissimum Nakai. Korean J. Medicinal Crop Sci. 14:354-359
  10. Kim SY, Choi HW, Kim CS, Sung JS, Lee JK, Bang JW (2006b) Cytogenetic Analyses of Astragalus speceis. Korean J. Medicinal Crop Sci. 14:250-254
  11. Kwak M, Kim MH, Won H, Park CW (2006) Variation of nuclear ribosomal ITS sequences of Polygonum section Persicaria (Polygonaceae) in Korea. Korean J. Plant Tax. 36:21-39 https://doi.org/10.11110/kjpt.2006.36.1.021
  12. Koo DH, Kim SY, Bang KH, Seong NS, Bang JW (2003) Cytogenetic analyses of Angelica plants using Feulgen staining and multicolor fluorescence in situ hybridization. Korean J. Plant Biotech. 30:123-127 https://doi.org/10.5010/JPB.2003.30.2.123
  13. Lapitan NLV (1992) Organization and evolution of higher plant genomes. Genome 35:171-181 https://doi.org/10.1139/g92-028
  14. Lee TB (1985) Illustrated Flora of Korea. Hyangmoonsa Co., Seoul., p. 305-313
  15. Lee YN (1971) Chromosome numbers of flowering plants in Korea. J. Kor. Res. Inst. Better Living 8:21-31
  16. Levan A, Frekga K, Sandberg A (1964) Nomenclature for centromeric position in chromosomes. Hereditas 52:201-220 https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  17. Nahar KK, Alam SS (1998) Differential chromosome banding in Persicaria posumbu (Hamilton ex. D Don) H Gross (Polygonaceae). Bangl. J. Bot. 27:103-107
  18. Park JH (2002) The Encyclopedia of Chinese Crude Drugs. Shinilsangsa Publishing Co., Seoul., p. 1-973
  19. Park JH (2004) Medicinal Plants of Korea. Shinilsangsa Publishing Co., Seoul., p. 988-1017
  20. Schwarzacher T, Leitch AR, Bennet MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann. Bot. 64:315-4324 https://doi.org/10.1093/oxfordjournals.aob.a087847
  21. Suh Y, Kim S, Park CW (1997) A phylogenetic study of Polygonum sect. Tovara (polygonaceae) based on ITS sequences of nuclear ribosomal DNA. J. Plant Biol. 40:47-52 https://doi.org/10.1007/BF03030320
  22. Yasuda K, Yamaguchi H (2005) Genetic diversity of vegetable water pepper (Persicaria hydropiper (L.) Spach) as revealed by RAPD markers. Breed. Sci. 55:7-14 https://doi.org/10.1270/jsbbs.55.7