Studies on the Physicochemical Characteristics and NDMA Formation of Orostachys japonicus A. Berger

와송의 이화학적 특성 및 NDMA 생성에 관한 연구

  • Choi, Sun-Young (Dept. of Home Economics Education, Education Research Institute, Gyeongsang National University) ;
  • Kim, Jeong-Gyun (Dept. of Marine Bioscience. Gyeongsang National University) ;
  • Sung, Nak-Ju (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 최선영 (경상대학교 가정교육과 교육연구원) ;
  • 김정균 (경상대학교 해양생물이용학부) ;
  • 성낙주 (경상대학교 식품영양학과 농업생명과학연구원)
  • Published : 2008.06.30

Abstract

The purpose of this study was to analyze the changes in physicochemical characteristics and NDMA formation of Orostachys japonicus A. Berger according to harvest times. The results showed moisture, carbohydrate, crude fiber and crude protein contents of <$48.5{\pm}1.4%$, $38.2{\pm}1.2%$, $l5.8{\pm}0.4%$ and $13.2{\pm}0.6%$ respectively. The total mineral content was 4,817.1 mg/100 g, where Ca was highest($2,577.0{\pm}3.2$ mg/100 g), followed by K, Mg, Na, P and Fe. The composition amino acid of O. japonicus A. Berger showed high levels of glutamic acid and aspartic acid. And among the five free sugars detected, galactose and glucose were most abundant at $32.2{\pm}0.02$ mg/100 g and $25.3{\pm}0.1O$ mg/100 g, respectively. Upon comparing O. japonicus A. Berger extracts that came from plants harvested during $August{\sim}October$, total phenolic compound($2,703.l{\pm}5.2l{\sim}2,428.0{\pm}3.52$ mg/100 g) and flavonoid($634.2{\pm}2.33{\sim}1,324.6{\pm}1.87$ mg/100 g) contents were higher in the methanol extract than in the water extract. Also, within a reaction system, nitrite scavenging ability and NDMA inhibition were most effective at pH 2.5, and increased in proportion to the extract concentration. Finally, the methanol extract of O. japonicus A Berger harvested during $August{\sim}October$ had the highest nitrite scavenging and NDMA inhibition effects.

Keywords

References

  1. Zeisel, SH, daCosta, KA and laMont, JT. Mono-, di- and trimethylamine in human gastric juice, potential substrates for nitrosodimethylamine formation. Carcinogenesis. 9:179-181. 1988 https://doi.org/10.1093/carcin/9.1.179
  2. Lijinsky, W. Significance of in vivo formation of N-nitroso compounds. Oncology. 37:223-226. 1980 https://doi.org/10.1159/000225440
  3. Marvish, SS. Kinetics of dimethylamine nitrosation in relation to nitrosamine carcinogenesis. J. Natl. Cancer Inst. 44: 633-639. 1970
  4. Kato, H, Lee, IE, Cheyen, NV, Kim, SB and Hayase, F. Inhibitory of nitrosamine formation by nondialyzablemelanoidins. Aric. Biol. Chem. 51:1333-1336. 1987 https://doi.org/10.1271/bbb1961.51.1333
  5. Conney, RV and Ross, PD. N-nitrosation and N-nitration of morpholine by nitrogen dioxide in aqueous solution: Effects of vanillin and related phenols. J. Agric. Food Chem. 35: 789-796. 1998 https://doi.org/10.1021/jf00077a036
  6. Ning, ZX, Zhang, SH, Gao, JH, Mo, L, Chen, H, Hang, QB and Cai, YC. Elimanation of active free radicals and nitrite by some fresh fruits and vegetables. Food Fermentation Industry. 2:31-35. 1995
  7. Song, MH, Lee, SJ, Shin, JH, Choi, SY and Sung, NJ. Effect of the N-nitrosodimethylamine formation in ascorbate and phenolic portions from citrus juice. Kor. J. Food Nutr. 15: 97-103. 2002
  8. Choi, SY. Effect of green tea and Maesil(Prunus mume) extracts on endogenous formation of N-nitrosamine in humans. MS. Thesis, Gyeongsang Nati. Uni., Jinju, 2001
  9. Hsu, HY, Chen, YP, Shen, SJ, Hsu, CS and Chen, CC. Oriental material medica: a concise guide, Califonia, 473-474. 1986
  10. Park, JC, Han, WD, Park, JR, Choi, SH and Choi, JW. Changes in hepatic drug metabolizing enzymes and lipid peroxidation by methanol extract and major compound of Orostachys japonicus. J. Ethnopharmacology. 102:313-318. 2005 https://doi.org/10.1016/j.jep.2005.06.023
  11. Park, HJ, Lim, SC, Lee, MS and Young, HS. Triterpene and steroids from Orostachys japonicus. Kor. J. Pharmacogn. 25:20-24. 1994
  12. Park, JG, Park, JC, Hur, JM, Park, SJ, Choi, DR, Shin, DY, Park, KY, Cho, HW and Kim, MS. Phenolic compounds from Orostachys japonicus having anti-HIV-1 protease activity. Nat. Prod. Sci. 6:117-121. 2000
  13. Gutfinger, T. Polyphenols in olive oil. J. Am. Oil Chem. Soc. 58:966-968. 1958 https://doi.org/10.1007/BF02659771
  14. Moreno, MIN, Isla, MIN, Sampietro, AR and Vattuone, MA. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J. Enthropharmacology. 71:109-114. 2000 https://doi.org/10.1016/S0378-8741(99)00189-0
  15. Kim, DS, Ahn, BW, Yeum, DM, Lee, DW, Kim, ST and Park, YH. Degardation of carcinogenic nitrosamine formation factor by natural food components. Bull. Kor. Fish Soc. 20:463-468. 1987
  16. Kang, YM. Functional characteristics and identification of Alkebia quinate extracts. MS. Thesis, Gyeongsang Nati. Uni., Jinju, 2004
  17. Na, GM, Han, HS, Ye, SH and Shin, JI. Physiological activity of medicinal plant extracts. Kor. J. Food Preservation. 11: 388-393. 2004
  18. Choi, U, Shin, DH, Chang, YS and Shin, JI. Screening of natural antioxidant from plant and their antioxidative effect. Kor. J. Food Sci. Technol. 24:142-148. 1992
  19. Chung, HJ and Noh, KL. Screening of electron donating ability, antibacterial activity and nitrite scavenging effect of some herbal extracts. J. Kor. Soc. Food Sci. 16:372-377. 2000
  20. Kang, YH, Park, YK and Lee, GD. The nitrite scavenging and electron donating ability of phenolic compounds. Kor. J. Food Sci. Technol. 28:232-239. 1996