Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Scavenging Strategy of Panax ginseng Against Formed Free Radicals Under Stress of Mercuric Chloride in Rattus norvegicus

  • Mahour, Kanhiya (Toxicology Laboratory, Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University) ;
  • Saxena, Prabhu N. (Toxicology Laboratory, Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University)
  • Published : 2008.06.30
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Abstract

Twenty five albino rats were divided into five groups for conducting this experiment. The first group was for positive control (Vitamin C, ascorbic acid), the second group was of Panax ginseng (10 mg/kg body weight) treated group after bio-activity assay, the third group was of mercuric chloride treated group (0.033 mg/kg body weight) based on calculating $LD_{50}$ 9.26 mg/kg body weight by probit analysis, the fourth group was of mercuric chloride (0.033 mg/kg body weight) followed by Panax ginseng (10 mg/kg body weight) and the fifth group was Panax ginseng (10 mg/kg body weight) followed by mercuric chloride (0.033 mg/kg body weight) treated group. The interval between intake of Panax ginseng and mercuric chloride was of 2 hours in groups, fourth and fifth respectively. Comparative free radical scavenging property of Panax ginseng was studied under three in vitro models (role model for calculating scavenging activity) viz. DPPH method (hydroxyl free radicals), Nitric oxide method (nitrile free radicals) and Lipid peroxidation (mercury free radicals).

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References

  1. Tiwari, A. K. : Imbalance in antioxidant defense and human diseases: multiple approach of natural antioxidants therapy. Curr Sci. 81, 1179-1187 (2001)
  2. Saxena, P. N., Mahour, K. and Kumar, A. : Protective effect of Panax ginseng extract on renal functions altered by mercuric chloride in albino rats. J Ginseng Res. 30, 100-105 (2006) https://doi.org/10.5142/JGR.2006.30.3.100
  3. Gasoo, S., Sunol, C., Sanfeliu, C., Rodriguez-Farre, E. and Cristofol, R. M. : Pharmacological characterization of the effects of methyl mercury and mercuric chloride a spontaneous noradrenaline release from rat hippocampus slices. Life Sci. 67, 1210-1231(2000)
  4. Finney, D. J. : Probit analysis. Cambridge University press, pp. 303 (1971)
  5. Ohkawa, H., Ohishi, N. and Yagi, K. : Assay for lipid peroxides in animal tissues by thio barbituric acid reaction. Anal Biochem. 95, 351(1979) https://doi.org/10.1016/0003-2697(79)90738-3
  6. Kumar, P. V., Shashidhara, S., Kumar, M. M. and Sridhara, B. Y. : Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity. J Pharm Pharmacol. 52, 891 (2000) https://doi.org/10.1211/0022357001774589
  7. Sreejayan, N. and Rao, M. N. A. : Free radical scavenging activity of curcuminoids. Drug Res. 46, 169 (1996)
  8. Sreejayan, N. and Rao, M. N. A. : Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol. 49, 105 (1997) https://doi.org/10.1111/j.2042-7158.1997.tb06761.x
  9. Shirwaikar, A. and Somashekar, A. P. : Anti-inflammatory activity and free radical scavenging studies of Aristolochia bracteolate Lam. Ind J Pharm. Sci. 65, 68 (2003)
  10. Green, L. C., Wagner, D. A., Glogowski, J., Skipper, P. L., Wishnok, J. S. and Tanncubaum, S. R. : Analysis of nitrate and $N^{15}$ in biological fluids. Anal Biochem. 126, 131 (1982) https://doi.org/10.1016/0003-2697(82)90118-X
  11. Marcocci, L., Maguire J. J., Droy-Lefaix, M. T. and Packer, L. : The nitric oxide scavenging property of Ginko biloba extracts EBG 761. Biochem Biophys Res Commun. 201, 748 (1994) https://doi.org/10.1006/bbrc.1994.1764
  12. Gutteridge, J. M. C. : Age pigments and free radicals: fluorescent lipid complexes formed by iron and copper containing proteins. Biochem Biophys Acta. 834, 144 (1985) https://doi.org/10.1016/0005-2760(85)90149-3
  13. Halliwell, B. : Super oxide-dependent formation of hydroxyl free radicals in the presence of iron chelates. FEBS Lett. 92, 321 (1978) https://doi.org/10.1016/0014-5793(78)80779-0
  14. Brraugghler, J. M., Duncan, C. A. and Chase L. R. : The involvement of iron in lipid peroxidaiton. Importance of ferrous and ferric ratio in initiation. J Biol Chem. 261, 10282 (1986)
  15. Pryor, W. A. : Oxy-radicals and related species, their formation, lifetimes and reactions. Ann Rev Physiol. 48, 657 (1986) https://doi.org/10.1146/annurev.ph.48.030186.003301
  16. Ialenti, A., Moncada, S. and Di Rosa, M. : Modulation of adjuvant arthritis by endogenous nitric oxide. Br J Pharmacol. 110, 701 (1993) https://doi.org/10.1111/j.1476-5381.1993.tb13868.x
  17. Ross, R. : The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362, 801-811 (1993) https://doi.org/10.1038/362801a0
  18. Marcocci, L., Packer, L., Droy-Lefaiz, M. T., Sekaki, A. and Gardes-Albert, M. : Antioxidant action of Ginko biloba extracts Egb761. Meth Enzymol. 234, 462-467 (1994) https://doi.org/10.1016/0076-6879(94)34117-6
  19. Sanchez-Moreno, C. : Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Tech Int. 8, 122-127 (2002)
  20. Blos, M. S. : Antioxidant determinations by the use of stable free radical. Nature 26, 1199-1220 (1958)