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Antioxidant Activities of Domestic Garlic (Allium sativum L.) Stems from Different Areas

산지별 국내산 마늘종의 항산화 성분과 항산화 활성 비교

  • 정지영 (창원전문대학 식품영양과) ;
  • 김창순 (창원대학교 식품영양학과)
  • Published : 2008.08.30

Abstract

Antioxidant compounds and activities were investigated for both northern (Uiseong) and southern ecotype (Namhae) domestic garlic stems. The contents of chlorophyll, carotenoid, vitamin C, phenolic compounds and allicin in garlic stems were measured. Antioxidant activities of methanol and ethanol extracts of garlic stems were compared determining lipid peroxidation on the linoleic acid system, electronic donating ability (EDA), superoxide dismutase (SOD)-like activity and nitrite scavenging ability (NSA). The amounts of chlorophyll, carotenoid, phenolic compounds and allicin in northern ecotype were significantly higher, but vitamin C content was lower than those of southern ecotype garlic stems. The allicin contents of garlic stems and garlic bulbs were $26.1{\pm}1.0{\sim}28.2{\pm}0.9$ and $33.2{\pm}0.7{\sim}33.5{\pm}0.8\;mg%$, respectively. All antioxidant activities were higher in methanol extracts of garlic stems than in ethanol extracts and were higher with northern ecotype than those with southern ecotype extract. The SOD-like activity of garlic stem extract was higher than that of garlic bulbs while EDA, lipid peroxidation and NDA of garlic stem extracts were lower than those of garlic bulbs. Antioxidant activities of garlic stems were more than 50% of garlic bulbs.

마늘의 재배 부산물인 마늘종을 산지별로 나누어 항산화 관련 물질을 측정하고 용매 추출물의 항산화력 및 아질산염 소거능을 조사하여 마늘종의 소비 확대를 장려할 수 있는 기초자료를 마련하고자 하였다. 항산화 성분 중 클로로필, 카로티노이드, 페놀화합물, allicin 함량은 한지형 마늘종이 많았고, 비타민 C 함량은 난지형 마늘종이 조금 많았다. 마늘은 마늘종에 비해 클로로필, 카로티노이드, 비타민 C 함량은 유의적으로 크게 낮았으나 페놀화합물과 allicin 함량은 유의적으로 높았다. 항산화 효능은 추출용매나 재배지역의 기후에 따라 영향을 받는 것으로 ethanol 추출물보다 methanol 추출물이, 난지형 마늘종보다 한지형 마늘종이 더 높았다. 마늘종은 마늘에 비해서 전자공여능, linoleic acid system에서의 항산화능, 아질산염 소거능이 낮았지만 마늘 항산화 효능의 50% 이상을 보였으며, SOD 유사활성은 마늘보다 유의적으로 높게 나타났다. 본 연구결과들로 마늘종도 마늘과 마찬가지로 항산화 효능이 있고 한지형이 난지형보다 더 높은 항산화 효능을 가지는 것으로 밝혀졌다. 따라서 마늘종도 기능성 식품소재로서 충분히 활용가치가 있다고 보이므로 이를 다양한 제품 개발에 이용할 수 있는 방법들도 추후 연구되어야 할 것이다.

Keywords

References

  1. Sharma KK, Sharma AL, Dwivedi KK, Sharma PK. 1976. Effect of raw and boiled garlic on blood cholesterol in butter fat lipidemia. The Ind J Nutr Dietet 13: 7-11
  2. Hoong MS. 1992. The culture of food in Korea. Kyomoonsa, Seoul. p 79
  3. Park PS, Lee MY. 1992. The effects of onion and garlic on copper-phenanthroline complex induced DNA degradation. J Korean Soc Food Nutr 21: 367-371
  4. Kim SH, Kim JO, Lee SH, Park KY, Park HJ, Chung HY. 1991. Antimutagenic compounds identified from the chloroform fraction of garlic (Allium sativum). J Korean Soc Food Nutr 20: 253-259
  5. Rho SN, Han JH. 2000. Cytotoxicity of garlic and onion methanol extract on human lung cancer cell lines. J Korean Soc Food Sci Nutr 29: 870-874
  6. Sheo HJ. 1999. Effects of garlic on the blood lipids and other serum components in rats. J Korean Soc Food Sci Nutr 28: 1339-1348
  7. Jain RC, Vyas CR. 1975. Garlic in alloxan-induced diabetic rabbits. Am J Clin Nutr 28: 6865-6874
  8. Kim HJ, Kim GJ, Jun TW, Lee ES, Lee YS, Han OK. 2002. Hepatoprotective effects of semisulcospira libertina and garlic on the liver damage induced by carbon tetrachloride in rats. J Korean Soc Food Sci Nutr 31: 58-520 https://doi.org/10.3746/jkfn.2002.31.3.516
  9. Chung KS, Kim JY, Kim YM. 2003. Comparison of antibacterial activities of garlic juice and heat-treated garlic juice. Korean J Food Sci Technol 35: 540-543
  10. Kim MH, Kim SY, Shin WS, Lee JS. 2003. Antimicrobial activity of garlic juice against Escherichia coli O157:H7. Korean J Food Sci Technol 35: 752-755
  11. Nuttakaan L, Viboon R, Nantaya C, Janusz MG. 2006. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations. Nutrition 22: 266-274 https://doi.org/10.1016/j.nut.2005.05.010
  12. Iqbal S, Bhanger MI. 2005. Stabilization of sunflower oil by garlic extract during accelerated storage. Food Chem 100: 246-254 https://doi.org/10.1016/j.foodchem.2005.09.049
  13. Yang SY, Yu SO, Kim TC, Kim BW, Park YJ, Cho JY, Kim YM, Heo BG. 2005. Compare to the component contents and anti-oxidation activities between Goheung native garlic variety and introductions. J Korean Soc Plant People Environ 8: 1-5
  14. Lee EJ, Kim KS, Jung HY, Kim DH, Jang HD. 2005. Antioxidant activities of garlic (Allium sativum L.) with growing districts. Food Sci Biotechnol 14: 123-130
  15. Jo JS. 1990. Food materials. Gijeunyungusa, Seoul. p 154-155
  16. 白合科菜蔬栽培技術. 1994. 華甲紀念著刊行委員會. p 14
  17. Lee MK, Park JS, Na HS. 2005. Proximate compositions of green garlic powder and microbiological properties of bread with green garlic. Korean J Food Preserv 12: 95-100
  18. Kim MY, Chung SK. 1997. Analysis of nutritional and volatile flavor compounds of garlic shoot. Kor J Post-harvest Sci Technol Argic Products 4: 61-68
  19. Kim MY, Choi SW, Chung SK. 2000. Antioxidative flavonoids from the garlic (Allium sativum L.) shoot. Food Sci Biotechnol 9: 199-203
  20. Kim MY, Kim YC, Chung SK. 2005. Identification and in vitro biological activities of flavonols in garlic leaf and shoot: inhibition of soybean lipoxygenase and hyaluronidase activities and scavenging of free radicals. J Sci Food Agric 85: 633-640 https://doi.org/10.1002/jsfa.1899
  21. White RC, Jones IK, Gibbs E. 1963. Determination of chlorophylls, chlorophyllides, pheophytins and pheophorbides in plant materials. J Food Sci 28: 431-438 https://doi.org/10.1111/j.1365-2621.1963.tb00222.x
  22. AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Arington, VA, USA
  23. Sood SP, Sartori LE, Wittmer DP, Haney WG. 1976. High pressure liquid chromatographic determination of ascorbic acid in selected foods and multivitamin products. Anal Chem 48: 796-802 https://doi.org/10.1021/ac60370a025
  24. Benvenuti S, Pellati F, Melegari M, Bertelli D. 2004. Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of rubus, ribes, and aronia. J Food Sci 69: FCT164-169 https://doi.org/10.1111/j.1365-2621.2004.tb13352.x
  25. Talia M, Irina S, Guy F, Lev W, David M, Meir W, Aharon R. 2002. A spectrophotometric assay for allicin, alliin, and alliinase (alliin lyase) with a chromogenic thiol: reaction of 4-mercaptopyridine with thiosulfinates. Anal Biochem 307: 76-83 https://doi.org/10.1016/S0003-2697(02)00010-6
  26. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  27. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  28. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidines. Agric Biol Chem 51: 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  29. Osawa T. 1981. A novel type of antioxidant isolated from leaf was of Eucalypyus leaves. Agric Biol Chem 45: 735-739 https://doi.org/10.1271/bbb1961.45.735
  30. Byun PH, Kim WJ, Yoon SK. 2001. Effects of extraction conditions on the functional properties of garlic extracts. Korean J Food Sci Technol 33: 507-513
  31. Tanielian C, Wolff C. 1988. Mechanism of physical quenching of singlet molecular oxygen by chlorophylls and related compounds by biological interest. Photochem Photobiol 48: 277-280 https://doi.org/10.1111/j.1751-1097.1988.tb02821.x
  32. Endo Y, Usuki R, Kaneda T. 1985. Prooxidant activities of chlorophyll and their decomposition products on the photooxidation of methyl linoleate. J Am Oil Chem Soc 61: 781-784 https://doi.org/10.1007/BF02672135
  33. Kim JW. 1993. Medical application of carotenoids. Korean J Food and Nutrition 6: 231-244
  34. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol 37: 233-240
  35. Anna MN, Riitta PP, Marjukka A, Kirsi-Marja OC. 2003. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chem 81: 485-493 https://doi.org/10.1016/S0308-8146(02)00476-4
  36. Stoll A, Seeback E. 1951. Chemical investigation on alliin, the specific principle of garlic. Advan Enzymol 11: 377-379
  37. Block E, Naganathan S, Putnam D, Zhao SH. 1992. Allium chemistry: HPLC analysis of thiosulfinates from onion, garlic, wild garlic (Ramsoms), leek, scallion, shallot, elephant (great headed) garlic, chive, and Chinese chive. Uniquely high allyl ratio in some garlic samples. J Agric Food Chem 40: 2418-2430 https://doi.org/10.1021/jf00024a017
  38. Bronitz MH, Pascale JV. 1971. Flavor components of garlic extract. J Agric Food Chem 19: 273-278 https://doi.org/10.1021/jf60174a007
  39. Saghi AR, Mann LK, Bernhard RA, Jacobsen JV. 1964. Determination of aliphatic mono- and disulfides in allium by gas chromatography and their distribution in the common food species. Pro Am Soc Hort Sci 84: 386-391
  40. Ziegler SJ, Sticher O. 1989. HPLC of s-alk(en)yl-L-cysteine derivatives in garlic including quantitive determination of (+)-s-allyl-L-cysteine sulfoxide (alliin). Planta Med 55: 372-378 https://doi.org/10.1055/s-2006-962031
  41. Jansen H, Muller B, Knobloch K. 1987. Allicin characterization and its determination by HPLC. Planta Med 53: 559-565 https://doi.org/10.1055/s-2006-962811
  42. Fugjiwara M, Yoshimura M, Tsuno S. 1955. "Allithiamine" A newly found derivatives vitamin B. III on the allicin homologues in the plants of the allium species. J Biochem 42: 591-595
  43. Joan H, Larry L, Grace H, Peter H. 1995. A spectrophotometric method for quantitative determination of allicin and total garlic thiosulfinates. Anal Biochem 225: 157-160 https://doi.org/10.1006/abio.1995.1124
  44. Talia M, Aharon R, David M, Lev W, Meir W. 1998. A spectrophotometric assay for allicin and alliinase (Alliin lyase ) activity: reaction of 2-nitro-5-thiobenzoate with thiosulfinates. Anal Biochem 265: 317-325 https://doi.org/10.1006/abio.1998.2924
  45. Shin JH, Ju JC, Kwen OC, Yang SM, Lee SJ, Sung NJ. 2004. Physicochemical and physiological activities of garlic from different area. Korean J Food Nutr 17: 237-245
  46. Kim SJ, Han D, Moon KD, Rhee JS. 1995. Measurement of superoxide dismutase-like activity of natural antioxidants. Biosci Biotech Biochem 59: 822-826 https://doi.org/10.1271/bbb.59.822
  47. Chatterjee IB, Nandi A. 1991. Ascorbic acid: a scavenger of oxyradicals. Indian J Biochem Biophysic 28: 233-236
  48. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632
  49. Im KJ, Lee SK, Park DK, Rhee MS, Lee JK. 2000. Inhibitory effects of garlic on the nitrosation. Agric Chem Biotechnol 43: 110-115
  50. Chung SY, Kim NK, Yoon S. 1999. Nitrite scavenging effect of methanol fraction obtained from green yellow vegetable juices. J Korean Soc Food Sci Nutr 28: 342-347
  51. Shin JH, Lee JY, Cho HS, Lee SJ, Jung KH, Sung NJ. 2004. Screening of effective factor to inhibition of NDMA formation in Yuza (Citrus junos). J Fd Hyg Safety 19: 126-131
  52. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239
  53. Kang JH, Ahn BW, Lee DH, Byun HS, Kim SB, Park YH. 1988. Inhibitory effects of ginger and garlic extracts on the DNA damage. Korean J Food Sci Technol 20: 287-292

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