Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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High Quality Green Tea Extract Production from Enzyme Treated Fresh Green Tea Leaves

효소를 이용한 녹차 생엽에서 고품질 녹차 추출물 생산

  • Published : 2008.08.30
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Abstract

Fresh green tea leaf extracts were prepared by different enzyme treatment conditions, such as concentration, treating time and treating temperature using complex enzyme, Rapidase TF, and then extracted for 30 min at $80^{\circ}C$ to investigate their physicochemical properties. The results showed that free sugar content in every sample tended to increase, especially glucose content was increased up to 7.25 times compared to the control. Total amino acid was barely affected by the enzyme treatment and caffeine content was increased with reaction temperature. Total polyphenol and total catechin content was increased according to the amount of enzyme added and reaction temperature. Regardless of enzyme treatment conditions, composition of catechins were epigallocatechin, epicatechin, epicatechin gallate and epigallocatechin gallate by descending order of the content. Gallic acid content increased up to 0.04% and $45^{\circ}C$ with no further significant changes thereafter. From the results above, we could conclude that a simple and new method to extract green tea materials directly from fresh green tea leaves with improved extract ratio may be introduced by adding $0.08{\sim}0.1%$ of Rapidase TF to heat treated fresh green tea leaves and keeping temperature at $37{\sim}45^{\circ}C$ for $180{\sim}240\;min$ in order to skip existing complicated procedures.

녹차 생엽으로부터 고품질의 녹차 추출물을 얻기 위해 복합효소 Rapidase TF를 이용하여 효소처리 농도, 효소처리시간 및 효소처리 온도를 달리하여 실시한 후 $80^{\circ}C$에서 30 min 간 가열한 추출액의 이화학적 성분 특성을 조사하였다. 효소처리에 의해 유리당 함량은 모든 처리구에서 증가하는 경향을 보였으며 특히 glucose의 함량은 대조구에 비하여 최대 7.25배 증가하는 것으로 나타났다. 총아미노산은 효소 처리에 의한 영향이 거의 없었으며, 카페인 함량은 효소처리 온도가 높아짐에 따라 증가하였다. 총폴리페놀 및 총카테킨 함량은 효소 첨가량이 증가하고 효소처리 온도가 높아짐에 따라 증가하였으며, 카테킨 조성은 효소처리 조건에 관계없이 EGC, EC, ECg 및 EGCg의 순으로 함유되어 있었다. Gallic acid 함량은 효소처리 농도 0.04%, 효소처리 온도 $45^{\circ}C$까지 증가하다 그 이상에서는 변화가 거의 없었다. 이상의 결과로부터 증열 처리된 녹차 생엽에 복합효소 Rapitase TF를 $0.08{\sim}0.1%$ 첨가 후 $37{\sim}45^{\circ}C$로 $180{\sim}240\;min$ 처리함에 의해 기존의 복잡한 가공공정을 거치지 않고 생엽에서 바로 추출이 가능하도록 함에 따라 공정이 간단하고 녹차성분 추출률이 향상된 새로운 형태의 차 추출물 제조방법이 될 수 있을 것으로 사료되었다.

Keywords

References

  1. Kim JT. 1996. Science and culture of tea. Borimsa Publishing Co., Seoul, Korea
  2. Nakabayashi T, Ina K, Sakata K. 1994. Chemistry and function of green, black and oolong tea. Kogagu Press, Kawasaki, Japan
  3. Miao Y L, Shi LH, Lei ZL, Huang JC, Yang JW, Yang YC, Sun QY, Chen DY. 2007. Effect of caffeine on in vivo and in vitro oocyte maturation in mice. Theriogenology 68: 640-645 https://doi.org/10.1016/j.theriogenology.2007.04.061
  4. Higgins GA, Grzelak ME, Pond AJ, Cohen-Williams ME, Hodgson RA, Varty GB. 2007. The effect of caffeine to increase reaction time in the rat during a test of attention is mediated through antagonism of adenosine $A_{2A}$ receptors. Behav Brain Res 185: 32-42 https://doi.org/10.1016/j.bbr.2007.07.013
  5. Keast RS, Riddell LJ. 2007. Caffeine as flavor additive in soft-drinks. Appetite 49: 255-259 https://doi.org/10.1016/j.appet.2006.11.003
  6. Matsuzaki TL, Hara Y. 1985. Antioxidative activity of the leaf catechins. J Agric Chem Soc J apan 59: 129-134
  7. Yoshioka H, Sugiura K, Kawahara R, Hujita T, Makino M, Kamiya M, Tsuyumu S. 1991. Formation of radicals and chemiluminescence during the autoxidation of the catechins. Agric Biol Chem 55: 2717-2723 https://doi.org/10.1271/bbb1961.55.2717
  8. Song JM, Park KD, Lee KH, Byun YH, Park JH, Kim SH, Kim JH, Seong BL. 2007. Biological evaluation of anti-influenza viral activity of semi-synthetic catechin derivatives. Antivir Res 76: 178-185 https://doi.org/10.1016/j.antiviral.2007.07.001
  9. Khan SM, Kour G. 2007. Subacute oral toxicity of chlorpyriphos and protective effect of green tea extract. Pestic Biochem Phys 89: 118-123 https://doi.org/10.1016/j.pestbp.2007.04.005
  10. Mohan KV, Gunasekaran P, Varalakshmi E, Hara Y, Nagini S. 2007. In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells. Cell Biol Int 31: 599-608 https://doi.org/10.1016/j.cellbi.2006.11.034
  11. Higdon JV, Frei B. 2003. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. Crit Rev Food Sci Nutr 43: 89-143 https://doi.org/10.1080/10408690390826464
  12. Tang S, Kerry JP, Sheehan D, Buckley DJ, Morrissey PA. 2001. Antioxidative effect of added tea catechins on susceptibility of cooked red meat, poultry and fish patties to lipid oxidation. Food Res Int 34: 651-657 https://doi.org/10.1016/S0963-9969(00)00190-3
  13. Lee JW, Do JH. 2005. Market trend of health functional food and prospect of ginseng market. J Ginseng Res 29: 206-214 https://doi.org/10.5142/JGR.2005.29.4.206
  14. Wang L-F. 2000. Effects of processing and storage on flavanols and sensory qualities of green tea beverage. PhD Dissertation. Cornell University, NY. p 13-14
  15. Ikegaya K, Takayanagi H, Anan T. 1990. Quantitative analysis of tea constituents. Bull Natl Res Tea 71: 43-74
  16. Lee LS, Cha HS, Park JD, Jang DJ, Kim SH. 2008. Physicochemical properties of mushroom (Flammulina velutipes) cultivated with green tea. J Korean Soc Food Sci Nutr 37: 190-194 https://doi.org/10.3746/jkfn.2008.37.2.190
  17. SAS/STATTM User's Guide Release. 1988. SAS Institute, Cary, NC, USA
  18. Kim CM, Choi JH, Oh SK. 1983. Chemical change of major tea constituents during tea manufacture. J Korean Soc Food Nutr 12: 99-104
  19. Lu MJ, Chen C. 2008. Enzymatic modification by tannase increases the antioxidant activity of green tea. Food Res Int 41: 130-137 https://doi.org/10.1016/j.foodres.2007.10.012
  20. Kim SH, Han DS, Park JD. 2004. Changes of some chamical compounds of Korean (Posong) green tea according to harvest periods. Korean J Food Sci Technol 36: 542-546
  21. Kirishnamurthi KK. 1993. Proceedings of international seminar on green tea. Korean Society of Food Science and Technology. Seoul, Korea
  22. Battestin V, Macedo GA, De Freitas VAP. 2008. Hydrolysis of epigallocatechin gallate using a tannase from Paecilomyces variotii . Food Chem 108: 228-233 https://doi.org/10.1016/j.foodchem.2007.10.068
  23. Chen ZY, Chan PT. 1996. Antioxidant activity of green tea catechins in canola oil. Chem Phys Lipids 82: 163-172 https://doi.org/10.1016/0009-3084(96)02587-X
  24. Lekha PK, Lonsane BK. 1997. Production and application of tannin acyl hydrolase. Adv Appl Microbiol 44: 215-260 https://doi.org/10.1016/S0065-2164(08)70463-5

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