Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Changes in Enzymatic Activities during Eoyukjang Fermentation

어육장 발효 시 생성되는 효소의 활성 변화

  • Ham, Soo-Nam (Department of Food Science and Technology, Seoul National University of Technology) ;
  • Kim, Sang-Woo (Department of Food Science and Technology, Seoul National University of Technology) ;
  • Lee, Jae Hwan (Department of Food Science and Technology, Seoul National University of Technology) ;
  • Chang, Pahn-Shick (Department of Food Science and Technology, Seoul National University of Technology)
  • 함수남 (서울산업대학교 식품공학과) ;
  • 김상우 (서울산업대학교 식품공학과) ;
  • 이재환 (서울산업대학교 식품공학과) ;
  • 장판식 (서울산업대학교 식품공학과)
  • Published : 2008.06.30
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Abstract

Eoyukjang is a traditional sauce-type of Korean food that is similar to a soybean sauce made from fermented soybeans, and it is produced from a fermented mixture of sea foods, meats, and meju (soybean paste). This study examined periodical changes in the enzymatic activities of ${\alpha}$-amylase, esterase, ${\beta}$-glucosidase, protease, lipase, and lipoxygenase within the culture broth and solids of eoyukjang during 1 year of fermentation. The eoyukjang solids had 234-532% higher protein content than the culture broth. The specific activities of ${\alpha}$-amylase, esterase, ${\beta}$-glucosidase, and protease increased in both the culture broth and solids. Particularly, in the culture broth, ${\alpha}$-amylase, esterase, ${\beta}$-glucosidase, and protease activities rapidly increased (3- to 8-fold) until 10 months of fermentation, and then drastically decreased. However, the activities of lipase and lipoxygenase in both the culture broth and solids were less than 0.05 unit/mg of protein, respectively, throughout fermentation; thus, their activity levels were low and changed little over the 12 months. Overall, while the solids had higher protein content than the culture broth, the broth had greater enzyme activity levels during eoyukjang preparation.

한국의 전통 한국의 전통적인 식품 중, 대표적인 장류로서 반가 식품에 해당하는 수조육류와 어패류를 첨가하여 제조한 어육장(규합총서의 방법)을 발효시키되, 2달 간격으로 발효기간에 따라 ${\alpha}$-amylase, esterase, ${\beta}$-glucosidase, lipase, lipoxygenase 및 protease의 활성을 각각 측정하여 경시적 변화를 관찰하였다. 어육장을 액체와 고체시료로 나누어 이상에서 열거한 효소들의 활성을 측정한 결과, lipase 및 lipoxygenase의 비활성도는 모두 0.05unit/mg protein 이하로서 효소 활성도가 낮은 수준이었으며, 1년동안의 발효·숙성기간 중 비활성 증감변화도 미미하였다. 반면,${\alpha}$-amylase, esterase, ${\beta}$-glucosidase 및 protease는 숙성기간에 따라 효소의 활성이 증가하는 것을 확인할 수 있었고, 특히 10개월째에는 액체시료의 경우 4종류 효소의 비활성이 급격히 증가하였다가 감소하는 것을 확인할 수 있었다. 전반적으로 고체시료의 단백질 함량은 액체시료보다 높았지만, 액체시료의 비활성이 고체시료보다 2-5배 이상 높은 결과를 확인하였다.

Keywords

References

  1. Lee CH, Kwon TW. Introduction to Korean Foods. Korea University Press, Seoul, Korea. pp. 239-263 (2003)
  2. Cho HJ, Kim SC. A study on the contents of fatty acid and amino acid of Öyukjang. J. Natural Sci. 12: 119-120 (1995)
  3. Kim JS. A comparative study of physicochemical characteristics of soy sauce and Oyukjang during storage at different ripening temperatures. MS thesis, Kyung Hee University, Seoul, Korea (2005)
  4. Yoon SS. A Research on the History of Korean Food Culture. Shinkwang Publishing Co., Seoul, Korea. p.247 (1993)
  5. Oh HS. A bibliographical study on meat food in Korea. MS thesis, Hanyang University, Seoul, Korea (2002)
  6. Yoon MK, Choi AR, Cho IH, You MJ, Kim JW, Cho MS, Lee JM, Kim YS. Characterization of volatile components in Öyukjang. Korean J. Food Sci. Technol. 39: 366-371 (2007)
  7. Lim CL, Lee JM, Kim JW, You MJ, Kim YS, Noh BS. Comparison of volatile components in Ôyukjang and commercial soy sauce. Korean J. Food Culture 22: 383-387 (2007)
  8. Lowry RR, Tinsley IJ. Colorimetric analysis of fatty acids by cupric sulfate. J. Am. Oil Chem. Soc. 53: 470-478 (1976) https://doi.org/10.1007/BF02636814
  9. Shipe WF, Senyk GF, Fountain KB. Modification of cupric sulfate method for the analysis of free fatty acids. J. Dairy Sci. 63: 193-201 (1980) https://doi.org/10.3168/jds.S0022-0302(80)82913-4
  10. Bandani AR, Balvasi A. Comparison of $alpha$-amylase activity in larval stages of flour beetles, Tribolium confusum (Coleoptera: Tenebionidae). Commun. Agric. Appl. Biol. Sci. 71: 537-541 (2006)
  11. Kwon OT. Production of functional monoglyceride by esterasecatalyzed esterification in AOT-isooctane reversed micelles. MS thesis, Seoul National University of Technology, Seoul, Korea (2004)
  12. Gacesa P, Hubble J. Enzyme Technology. Taylor and Francis Press, London, UK. pp. 45-64 (1987)
  13. Son YJ, Sul OJ, Chung DK, Han IS, Choi YJ, Jeong CS. Isolation and characterization of Trichoderma sp. C-4 producing cellulase. Korean J. Appl. Microbiol. Biotechnol. 4: 346-353 (1997)
  14. Lee EJ. Characterization of protease from Calotropis procera R. Br.. MS thesis, Seoul National University of Technology, Seoul, Korea (2000)
  15. Han DS. Characteristics of lipase from Candida rugosa and hydrolysis of triglycerides by the enzyme in AOT-isooctane reversed micellar system. PhD thesis, Korea Advanced Institute of Science and Technology, Seoul, Korea (1986)
  16. Luisi PL, Straub BE. Acidities and Basicities in Reversed Micellar Systems. Plenum Press, New york, NY, USA. p. 81 (1984)
  17. Boyes S, Perera C, Young H. Kiwifruit lipoxygenase: Preparation and characteristics. J. Food Sci. Technol. 57: 1390-1398 (1995)
  18. Bradford M. A rapid and sensitive methods for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal. Biochem. 72: 248-254 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  19. Mok CK, Song KT, Lee JY, Park YS, Lim SB. Changes in microorganisms and enzyme activity of low salt soybean paste (Doenjang) during fermentation. Food Eng. Prog. 9: 112-117 (2005)
  20. Rhee CH, Lee JB, Jang SM. Changes of microorganisms, enzyme activity and physiological functionality in the traditional Deonjang with various concentrations of Lentinus edodes during fermentation. J. Korean. Soc. Agric. Chem. Biotechnol. 43: 277-284 (2000)
  21. Oh JH. The effects of $\beta$-glucosidase addition on genistein contents and physicichemical characteristics in Doenjang. MS thesis, Kookmin University, Seoul, Korea (2003)
  22. Yoon S, Kim JS. Isoflavone contents and $\beta$-glucosidase activities of soybeans, Meju, and Doenjang. Korean J. Food Sci. Technol. 31: 1405-1409 (1999)
  23. Yang SO, Chang PS, Lee JH. Isoflavone distribution and $\beta$-glucosidase activity in Cheonggukjang, a traditional Korean whole soybean-fermented food. Food Sci. Biotechnol. 15: 96-101 (2006)
  24. Kim DH, Song HP, Kim KY, Kim JO, Byun MW. A correlation between fibrinolytic activity and microflora in Korean fermented soybean products. J. Korean Soc. Food Sci. Nutr. 33: 41-46 (2004) https://doi.org/10.3746/jkfn.2004.33.1.041
  25. Oh JY, Kim YS, Shin DH. Changes in microorganisms and enzyme activities of low-salted Kochujang added with horseradish powder during fermentation. Korean J. Food Sci. Technol. 37: 463-467 (2005)
  26. Kim DH, Ahn HJ, Yook HS, Kim MJ, Sohn CB, Byun MW. Quality properties of gamma irradiated Samjang, seasoned soybean paste during storage. Korean J. Food Sci. Technol. 32: 396-401 (2000)
  27. Joo HK, Kim ND, Yoon KS. Changes of enzymatic activities during the fermentation of soybean-soypaste by Aspergillus sp. J. Korean Agric. Chem. Soc. 32: 295-302 (1989)
  28. Song YS, Kim JW, Jeon US, Sim YM, Jo HR. The study on lipoxygenase characteristics of common legumes. Injechongron 14: 917-926 (1998)
  29. Christopher JP, Pistoius EK, Axelrod B. Isolation of a third enzyme of soybean lipoxygenase. Biochim. Biophys. Acta 284: 54-62 (1972) https://doi.org/10.1016/0005-2744(72)90045-9
  30. Lee SY, Min YK, Park KH. Nutritional evaluation of naturally fermented soybean and the enzymatic activity changes during the preparation. Korean J. Food Sci. Technol. 15: 101-107 (1983)