Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Phytoestrogenic Effects of Combined Plant Extracts on the Change of Bone Metabolism of OVX Rats

복합 생약추출물의 식물성 에스트로겐 활성이 OVX rat에 미치는 골대사 변화

  • Kim, Soo-Nam (School of Veterinary Medicine, Kangwon National University) ;
  • Li, Yong-Chun (School of Veterinary Medicine, Kangwon National University) ;
  • Xu, Hong-De (School of Veterinary Medicine, Kangwon National University) ;
  • Yi, Dong-Geun (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Min-Seop (School of Veterinary Medicine, Kangwon National University) ;
  • Lee, Sung-Pyo (School of Veterinary Medicine, Kangwon National University) ;
  • Yi, Kwon-Taek (Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd.) ;
  • Lee, Jae-Kyoung (Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd.) ;
  • Kim, Jae-Soo (Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd.) ;
  • Kwon, Myung-Sang (School of Veterinary Medicine, Kangwon National University) ;
  • Chang, Pahn-Shick (Department of Food Science and Technology, Seoul National University of Technology) ;
  • Kwak, Bo-Yeon (Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd.)
  • 김수남 (강원대학교 수의학부대학) ;
  • 이용순 (강원대학교 수의학부대학) ;
  • 서홍덕 (강원대학교 수의학부대학) ;
  • 이동근 (강원대학교 수의학부대학) ;
  • 김민섭 (강원대학교 수의학부대학) ;
  • 이성표 (강원대학교 수의학부대학) ;
  • 이권택 ((주)내츄럴엔도텍) ;
  • 이재경 ((주)내츄럴엔도텍) ;
  • 김재수 ((주)내츄럴엔도텍) ;
  • 권명상 (강원대학교 수의학부대학) ;
  • 장판식 (서울산업대학교 식품공학과) ;
  • 곽보연 ((주)내츄럴엔도텍)
  • Published : 2008.06.30
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Abstract

This study examined the combined plant extracts (FGF271) of Estromon in ovariectomized (OVX) rats to determine whether Estromon's significant clinical improvement effects on menopausal symptoms are predominantly due to the phytoestrogenic action of the combined extracts. The results showed that all three FGF271-treated groups had significantly improved serum osteocalcin levels as compared to the control group (p<0.05). In addition, all FGF271- and Estromon-treated groups had increases in femoral bone mineral density (FBMD) (p<0.05), and the increase in the FGF271 group was dose-dependent. A pairwise comparison of the FGF271- and Estromon-treated groups receiving the same dosage of FGF271 indicated that there was no significant difference between the groups. Therefore, the FBMD increases that occurred in the Estromon groups were solely attributable to the phytoestrogenic effects of FGF271. It was conclude that the phytoestrogenic effects of Estromon, as shown in clinical studies, are predominantly caused by FGF271, the mixed extracts of Cynanchum wilfordii, Phlomis umbrosa, and Angelica gigas.

본 논문에서는 1년간의 임상연구 결과 폐경기 증상 개선효과와 골밀도 개선 효과가 관찰된 에스트로몬의 주요 성분인 복합 식물추출물인 FGF271의 식물성에스트로겐 효과를 검증하기 위해 난소를 절제한 rat 동물모델에서, FGF271과 에스트로몬을 비교 실험하였다. 시험군과 OVX 대조군간의 체중, 자궁, 신장, 및 간 무게 변화는 없는 것으로 나타나 FGF271이나 에스트로몬이 이들에 영향을 미치지 않았고, FGF271을 투여한 군에서 혈청 osteocalcin 농도가 통계적으로 유의한 개선효과를 보였다(p<0.05). 한편, FGF271과 에스트로몬 투여군에서 대퇴부 골밀도(Femoral Bone Mineral Density, FBMD)는 투여용량 73.5, 180, 440 mg/kg/ day 모두에서 유의하게 증가하였고(p<0.05), FGF271 투여군의 상대적 FBMD는 용량 의존적인 증가 경향을 나타내었다. FGF271 기준으로 동량 투여시(73.5 mg/kg/day; G4와 G8, 180 mg/kg/day; G5와 G9) 비교군 간에는 유의한 차이가 나타나지 않았다. 이로써 에스트로몬 투여군의 FBMD 증가가 에스트로몬의 주요성분인 백수오등 복합추출물인 FGF271만에 의해서 증가하였다고 판단할 수 있다. 이상의 결과, 임상시험에서 폐경기 증후군에 효능이 있는 것으로 확인된 에스트로몬의 식물성에스트로겐 효과는 Cynanchum wilfordii(백수오), Phlomis umbrosa(속단), Angelica gigas(당귀) 복합추출물로 구성된 FGF271에 기인하는 것으로 판단되었다.

Keywords

References

  1. Pelchetz PE. Hormonal treatment of postmenopausal women. New Engl. J. Med. 353: 1062-1071 (1994)
  2. Elfituri A, Sherif F, Elmahaishi M, Chrystyn H. Two hormone replacement therapy (HRT) regimens for middle-eastern postmenopausal women. Mauritas 52: 52-59 (2005)
  3. Rossouw JE, Anderson GL, Prentice RL, LaCroix AZ, Kooperberg C, Stefanick ML, Jackson RD, Beresford SA, Howard BV, Johnson KC, Kotchen JM, Ockene J. Writing groups for the women's health initiatives investigators. Risks and benefits of estrogen plus progestin in healthy postmenopausal woman: Principal results from the women's health initiatives randomized controlled trial. J. Am. Med. Assoc. 288: 321-333 (2002) https://doi.org/10.1001/jama.288.3.321
  4. Davison S, Davis SR. Hormone replacement therapy: Current controversies. Clin. Endocrinol. 58: 249-261 (2003) https://doi.org/10.1046/j.1365-2265.2003.01774.x
  5. Beral V, Banks E, Reeves G, Appleby P. Use of HRT and subsequent risk of cancer. J. Epidemiol. Biostatis. 4: 191-210 (1999)
  6. Persson I, Weiderpass E, Bergkvist L, Bergstrom R, Shchairer C. Risks of breast and endometrial cancer after estrogen and estrogen-progestin replacement. Cancer Cause Control 10: 253-260 (1999) https://doi.org/10.1023/A:1008909128110
  7. Rodriguez C, Calle EE, Coates RJ, Miracle-McMahill HL, Thun MJ, Heath CW Jr. Estrogen replacement therapy and fatal ovarian cancer. Am. J. Epidemiol. 141: 828-835 (1995) https://doi.org/10.1093/oxfordjournals.aje.a117518
  8. Lien LL, Lien EJ. Hormone therapy and phytoestrogen. J. Clin. Pharm. Ther. 21: 101-111 (1996) https://doi.org/10.1111/j.1365-2710.1996.tb00008.x
  9. Lukaczer D, Darland G, Tripp M, Liska D, Lerman RH, Sxhiltz B, Bland JS. Clinical effects of a proprietary combination isoflavone nutritional supplement in menopausal women: A pilot trial. Altern. Ther. Health M. 11: 60-65 (2005)
  10. Yoo HH, Kim T, Ahn S, Kim YJ, Kim HY, Piao XL, Park JH. Evaluation of the estrogenic activity of Leguminosae plants. Biol. Pharm. Bull. 28: 538-540 (2005) https://doi.org/10.1248/bpb.28.538
  11. Yap SP, Shen P, Li J, Lee LS, Yong EL. Molecular and pharmacodynamic properties of estrogenic extracts from the traditional Chinese medicinal herb, Epimedium. J. Ethnopharmacol. 113: 218-224 (2007) https://doi.org/10.1016/j.jep.2007.05.029
  12. Kenneth DR, Setchell. Phytoestrogens: The biochemistry, physiology, and implications for human health of soy isoflavones. Am. J. Clin. Nutr. 68(suppl): 1333S-46S (1998) https://doi.org/10.1093/ajcn/68.6.1333S
  13. Kim JS, Yoon S. Isoflavone contents and $beta$-glucosidase activities of soybeans, meju, and doenjang. Korean J. Food Sci. Technol. 31: 1405-1409 (1999)
  14. Albertazzi P, Pansini F, Bonaccorsi G, Zanotti L, Forini E, De Aloysio D. The effect of dietary soy supplementation on hot flushes. Obstet. Gynecol. 91: 6-11 (1998) https://doi.org/10.1016/S0029-7844(97)00597-8
  15. Albertazzi P, Pansini F, Bottazzi M, Bonaccorsi G, DE Aloysio D, Morton MS. Dietary soy supplementation and phytoestrogen levels. Obstet. Gynecol. 94: 229-231 (1999) https://doi.org/10.1016/S0029-7844(99)00275-6
  16. Mahady GB, Dog TL, Barrett ML, Chavez ML, Gardiner P, Ko R, Marles RJ, Pellicore LS, Giancaspro GI, Sarma DN. United States Phamacopeia review of the black cohosh case reports of hepatotoxicity. Menopause July 17: in press (2008)
  17. Lee KH, Lee DJ, Kim SM, Je SH, Kim EK, Han HS, Han IK. Evaluation of effectiveness and safety of natural plant extract (estromon) on perimenopausal women for 1 year. J. Korean Soc. Menopause 11: 16-26 (2005)
  18. Kim JS, Ha HK, KIM HJ, Lee JH, Song KY. Puheraria lobata Ohwi as an osteoporosis therapeutics. Korean J. Food Sci. Technol. 34: 710-718 (2002)
  19. Xie F, W CF, Zhang Y, Yao XS, Cheung PY, Chan AS, Wong MS. Increase in bone mass and bone strength by Sambucus williamsii Hance in ovariectomized rats. Biol. Pharm. Bull. 28: 1879-1885 (2005) https://doi.org/10.1248/bpb.28.1879
  20. Kim CS, Ha HK, Lee JH, Kim JS, Song KY, Park SW. Herbal Extract prevents bone loss in ovariectomized rats. Arch. Pharm. Res. 26: 917-924 (2003) https://doi.org/10.1007/BF02980200
  21. Peng S, Xia R, Fang H, Li F, Chen A, Zhang G, Qin L. Effect of epimedium-derived phytoestrogen on bone turnover and bone microarchitecture in OVX-induced osteoporotic rats. J. Hunazhong Univ. Sci. Technolog Med. Sci. 28: 167-170 (2008) https://doi.org/10.1007/s11596-008-0213-7
  22. Huang TH, Muhlbauer RC, Tang CH, Chen HI, Chang GL, Huang YW, Lai YT, Lin HS, Yang WT, Yang RS. Onion decreases the ovariectomy-induced osteopenia in young adult rats. Bone 42: 1154-1163 (2008) https://doi.org/10.1016/j.bone.2008.01.032