Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of ultrasonication on the analysis of sulforaphane content in vegetables

초음파 처리가 채소 중의 sulforaphane 분석에 미치는 영향

  • 최아름 (호서대학교 식품생물공학과) ;
  • 이건순 (한국농업대 생활과학과) ;
  • 채희정 (호서대학교 식품생물공학과)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of ultrasonication on the sulforaphane analysis in cruciferous vegetables was investigated by GC/MS. The ultrasonication of the analysis samples was carried out in dichloromethane as a solvent, which was followed by concentration in nitrogen gas, and the analysis of sulforaphane was performed using selective ion monitoring (SIM) at m/z 72, 160, 55 and 114. The content of sulforaphane was the highest in the extract of broccoli (149 ppm), and followed by cabbage (67.9 ppm) and radish (35.4 ppm). When the vegetable samples were shaked after ultrasonication, the extraction efficiency of sulforaphane was 2.7-fold enhanced, compared to the extraction by shaking. The result suggested that pretreatment including sonication can be used fer improving the extraction efficiency of sulforaphane.

초음파처리가 십자화과 채소의 sulforaphane 함량 분석에 미치는 영향을 GC/MS를 이용하여 검토하였다. 무, 알타리무, 순무, 양배추, 브로콜리를 각각 dichloromethane을 추출용매로 하여 초음파처리 후 용매 추출 및 질소 농축을 하고, m/z 72, 160, 55, 114을 선택이온측정법(selective ion monitoring, SIM)을 이용하여 분석하였다. 각 채소류의 sulforaphane 함량은 브로콜리가 149.0 ppm으로 가장 많았으며, 양배추(67.9 ppm), 무(35.4 ppm)의 순으로 높았다. Sulforaphane 추출율은 진탕교반(shaking)만 할 경우보다 초음파처리(sonication)후 진탕교반(shaking)을 할 경우 2.7배 높은 것으로 나타났다. 따라서 초음파추출공정이 sulforaphane의 추출효율 향상에 유효한 방법으로 판단된다.

Keywords

References

  1. Lee, S. M., Rhee, S. H. and Park, K. Y. (1997) Antimutagenic effect of various cruciferous vegetables in Salmonella assaying system. J. Food Hyg. Safety, 12, 321-327.
  2. Fenwick, G. R., Heaney, R. K. and Mawson, R. (1989) Glucosinolates. In : Toxicants of Plant Origin. Vol. II. Glycosides, Cheeke, P. R., (Ed.) CRC Press, Inc., USA, 277-281.
  3. Sparnins, V. L., Wattenberg, L. W. (1981) Enhancement of glutathione S-transferase activity of the mouse forestomach by inhibitors of benzo[a] pyrene-induced neoplasia of forestomach. J. Natl. Cancer Inst., 66, 769-771.
  4. Prakash, S. (1980) In "Brassica Crops and Wild Allies, Biology and Breeding". Japan Sci. Soc. Press., 151-163.
  5. Van Marrewijk, N. P. A. and Toxopeus, H. (1979) Proc. Eucarpia 'Cruciferae 1979' Conf. Wageningen., 47-56.
  6. Fenwick, G. R., Heaney, R. K. and Mulin, W. (1983) Glucosinolates and their breakdown products in food and food plants. Crit. Rev. Food Sci. Nutr., 18, 123-201.
  7. Kjacr, A. (1960) Naturally derived isothio cyanates (mustard oils) and their parent glucosides. Fortschr. Chem. Org. Naturst., 18, 122-176.
  8. Prochaska, H. J. and Talalay, P. (1988) Regulatory mechanisms of monofunctional and bifunctional anticarcinogenic enzyme inducers in murine liver. Cancer Res., 48, 4776-4782.
  9. Talaly, P., Fahey, J. W., Holtzclaw, W. D., Prestera, T. and Zhang, Y. (1995) Chemoprotecion against cancer by phase 2 enzyme induction. Toxicol., Lett., 82-83, 173-179. https://doi.org/10.1016/0378-4274(95)03553-2
  10. Zhang, Y., Talalay, P., Cho, C. G. and Posner, G. H. (1992) A major inducer of anticarcinogenic protective enzymes from broccoli: Isolation and elucidation of structure. Proc. Natl. Acad. Sci., 89, 2399-2403. https://doi.org/10.1073/pnas.89.6.2399
  11. Gamet-Payrastre L., LI. P., Lumeau, S., Cassar. G., Dupont, M. A., Chevolleau, S., Gasc, N., Tulliez, J. and terce, F. (2000) Sulforaphane a naturally occurring isothiocyante induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res., 60, 1426-1433.
  12. Bradfield, C. A. and Bjeldanes, L. F. (1987) High performance liquid chromatographic analysis of anticarcinogenic indoles in Brassica oleracea. J. Agric. Food. Chem., 35, 46-49. https://doi.org/10.1021/jf00073a010
  13. Whitty, J. P. and Bjeldanes, L. F. (1987) The effects of dietary cabbage on xenobiotic metabolizing enzymes and the binding of aflatoxin $B_{1}$ to hepatic DNA in rats. Food. Chem. Toxicol., 25, 581-587. https://doi.org/10.1016/0278-6915(87)90018-4
  14. Park, K. Y., Lee, K. I. and Rhee, S. H. (1992) Inhibitory effect of green-yellow vegetables on the mutagenicity in Salmonella assay system and on the growth of AZ-521 human gastric cancer cells. J. Food Sci. Nutr., 21, 149-153.
  15. Chung, H. S. and Youn, K. S. (2005) Effects of microwave, ultrasound and roasting pretreatments on hot water extraction of Acanthopanax senticosus.. Korean J. Food Preserv. 12, 146-150.
  16. Earnshaw, R. G. (1998) Ultrasound a new opportunity for food preservation. In : Ultrasound in Food Processing, Povey, M. J. W and Mason, T. J.(Ed.), Blackie Academic and Professional, London, England, 183-192.
  17. Kiyotaka, N., Toshiko, U., Ohki, H., Tsuyoshi, T., Toshihide, S. and Teruo, N. (2006) Evaporative light scattering analysis of sulforaphane in broccoli samples: Quality of vroccoli products regarding sulforaphane contents. J. Agric. Food. Chem., 54, 2479-2483. https://doi.org/10.1021/jf051823g
  18. Coogan, R. C., Wills, R. B. H. and Nguyen, V. Q. (2001) Pungency levels of white radish (Raphanus sativus L.) grown in different seasons in Australia. Food Chem., 72, 1-3. https://doi.org/10.1016/S0308-8146(00)00164-3
  19. Lee, J. M., Yoo, I. O. and Min, B. H. (1996) Effect of cultivars and cultural conditions on the pungnet principle contents in radish roots. J. Kor. Soc. Hort. Sci., 37, 349-356.
  20. Pereira, F. M. V., Rosa, E., Fahey, J. W., Stephenson, K. K., Carvalho, R. and Aires, A. (2002) Influences of temperature and ontogeny on the levels of glucosinolates in broccoli (Brassica oleracea var. italica) sprouts and their effect on the induction of mammalian phase 2 enzymes. J. Agric. Food. Chem., 50, 6239-6244. https://doi.org/10.1021/jf020309x
  21. Kim, M. R., Lee, K. J., Kim, J. H. and Sok, D. E. (1997) Determination of sulforaphane in cruciferous vegetables by SIM. J. Food Sci. Technol., 29, 882-887.
  22. Hao, L., Yuan, Q. and Xiao, Q. (2005) Purification of sulforaphane from Brassica oleracea seed meal using low-pressure column chromatography. J. Chromato. B., 828, 91-96. https://doi.org/10.1016/j.jchromb.2005.09.041

Cited by

  1. Optimum Extraction of Tricin and Tricin 4'-O-(threo-β-guaiacylglyceryl) Ether (TTGE) from Rice Hull (Oryza sativa L.) vol.44, pp.12, 2015, https://doi.org/10.3746/jkfn.2015.44.12.1923