한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제23권2호
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  • Pages.101-108
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  • 2008
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

Donor Cell Source (Miniature Pig and Landrace Pig) Affects Apoptosis and Imprinting Gene Expression in Porcine Nuclear Transfer Embryos

  • Park, Mi-Rung (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Hwang, In-Sun (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Shim, Joo-Hyun (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Moon, Hyo-Jin (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hoon (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Ko, Yeoung-Gyu (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Im, Gi-Sun (Division of Animal Biotechnology, National Institute of Animal Science, RDA)
  • 발행 : 2008.06.30
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초록

This study investigated the developmental ability and gene expression of somatic cell nuclear transfer embryos using ear skin fibroblast cells derived from miniature pig. When miniature pig (m) and landrace pig (p) were used as donor cells, there were no differences in cleavage (79.2 vs. 78.2%) and blastocyst rates (27.4 vs. 29.7%). However, mNT blastocysts showed significantly higher apoptosis rate than that of pNT blastocysts (6.1 vs. 1.7%) (p<0.05). The number of nuclei in pNT blastosysts was significantly higher than that of mNT (35.8 vs. 29.3) (p<0.05). Blastocysts were analyzed using Realtime RT-PCR to determine the expression of Bax-${\alpha}$, Bcl-xl, H19, IGF2, IGF2r and Xist. Bax-${\alpha}$ was higher in mNT blastocyst than pNT blastocyst (p<0.05). There was no difference in Bcl-xl between two NT groups. Bax-${\alpha}$/Bcl-xl was, however, significantly higher in mNT blastocyst compared to pNT. The expression of imprinting genes were aberrant in blastocysts derived from NT compared to in vivo blastocysts. H19 and IGF2r were significantly lower in mNT blastocysts (p<0.05). The expression of IGF2 and Xist was similar in two NT groups. However, imprinting genes were expressed aberrantly in mNT compared to pNT blastocysts. The present results suggest that the NT between donor cells derived from miniature pig and recipient oocytes derived from crossbred pig might affect reprogramming of donor cell, resulting in high apoptosis and aberrant expression patterns of imprinting genes.

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참고문헌

  1. Adams JM and Cory S. 1998. The Bcl-2 protein family: Arbiters of cell survival. Science 281:1322-1326 https://doi.org/10.1126/science.281.5381.1322
  2. Archer GS, Dindot S, Friend TH, Walker S, Zaunbrecher G, Lawhorn B and Piedrahita JA. 2003. Hierarchical phenotypic and epigenetic variation in cloned swine. Biol. Reprod. 69:430-436 https://doi.org/10.1095/biolreprod.103.016147
  3. Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke RA, Gavin WG, Overstrom EW and Echelard Y. 1999. Production of goats by somatic cell nuclear transfer. Nat. Biotechnol. 17:456-461 https://doi.org/10.1038/8632
  4. Barlow DP, Stoger R, Herrmann BG, Saito K and Schweifer N. 1991. The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus. Nature 349:84 https://doi.org/10.1038/349084a0
  5. Bartolomei MS, Zemel S and Tilghman SM. 1991. Parental imprinting of the mouse H19 gene. Nature 9:153-155
  6. Campbell KH, McWhir J, Ritchie WA and Wilmut I. 1996. Sheep cloned by nuclear transfer from a cultured cell line. Nature 7:64-66
  7. Cibelli JB, Campbell KH, Seidel GE, West MD and Lanza RP. 2002. The health profile of cloned animals. Nat. Biotechnol. 20:13-14 https://doi.org/10.1038/nbt0102-13
  8. Dai Y, Vaught TD, Boone J, Chen SH, Phelps CJ, Ball S, Monahan JA, Jobst PM, McCreath KJ, Lamborn AE, Cowell- Lucero JL, Wells KD, Colman A, Polejaeva LA and Ayares DL. 2002. Targeted disruption of the alpha 1,3 galactosyltransferase gene in cloned pigs. Nat. Biotechnol. 20:251-255 https://doi.org/10.1038/nbt0302-251
  9. De La Fuente R, O'Brien MJ and Eppig JJ. 1999. Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes. Hum. Reprod. 14:3060-3068 https://doi.org/10.1093/humrep/14.12.3060
  10. Dean W, Bowden L, Aitchison A, Klose J, Moore T, Meneses JJ, Reik W and Feil R. 1998. Altered imprinted gene methylation and expression in completely ES cell-derived mouse fetuses: association with aberrant phenotypes. Development 125:2273-2282
  11. Dinnyes A, De Sousa P, King T and Wilmut I. 2002. Somatic cell nuclear transfer: Recent progress and challenges. Cloning Stem. Cells. 4:81-90 https://doi.org/10.1089/153623002753632075
  12. Doherty AS, Mann MR, Tremblay KD, Bartolomei MS and Schultz RM. 2000. Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo. Biol. Reprod. 62:1526-1535 https://doi.org/10.1095/biolreprod62.6.1526
  13. Eggenschwiler J, Ludwig T, Fisher P, Leighton PA, Tilghman SM and Efstratiadis A. 1997. Mouse mutant embryos overexpressing IGF-II exhibit phenotypic features of the Beckwith- Wiedemann and Simpson-Golabi-Behmel syndromes. Genes. Develop. 11:3128-3142 https://doi.org/10.1101/gad.11.23.3128
  14. Feil R. 2001. Early-embryonic culture and manipulation could affect genomic imprinting. Trends. Mol. Med. 7:245-246
  15. Feugang JM, Van Langendonckt A, Sayoud H, Rees JF, Pampfer S, Moens A, Dessy F and Donnay I. 2003. Effect of prooxidant agents added at the morula/blastocyst stage on bovine embryo development, cell death and glutathione content. Zygote 11:107-118 https://doi.org/10.1017/S0967199403002144
  16. Gjorret JO, Knijn HM, Dieleman SJ, Avery B, Larsson LI and Maddox-Hyttel P. 2003. Chronology of apoptosis in bovine embryos produced in vivo and in vitro. Biol. Reprod. 69: 1193-1200 https://doi.org/10.1095/biolreprod.102.013243
  17. Haig D. 1992. Intragenomic conflict and the evolution of eusociality. J. Theore. Biol. 156:401-403 https://doi.org/10.1016/S0022-5193(05)80683-6
  18. Hao Y, Lai L, Mao J, Im GS, Bonk A and Prather RS. 2003. Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer. Biol. Reprod. 69:501-507 https://doi.org/10.1095/biolreprod.103.016170
  19. Hartshorn C, Riceand JE and Wangh LJ. 2002. Developmentallyregulated changes of Xist RNA levels in single preimplantation mouse embryos, as revealed by quantitative real-time PCR. Mol. Reprod. Dev. 61:425-436 https://doi.org/10.1002/mrd.10037
  20. Humpherys D, Eggan K, Akutsu H, Friedman A, Hochedlinger K, Yanagimachi R, Lander ES, Goluband TR and Jaenisch R. 2002. Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei. Proc. Natl. Acad. Sci. USA. 99:12889-12894
  21. Khosla S, Dean W, Brown D, Reik W and Feil R. 2001. Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes. Biol. Reprod. 64:918-926 https://doi.org/10.1095/biolreprod64.3.918
  22. Korsmeyer SJ. 1999. BCL-2 gene family and the regulation of programmed cell death. Cancer Res. 59:1693s-1700s
  23. Lai L and Prather RS. 2002. Progress in producing knockout models for xenotransplantation by nuclear transfer. Annual. Med. 34:501-506 https://doi.org/10.1080/078538902321117706
  24. Lee SU, Ock SA, Cho SK, Jeon BG, Kang TY, Balasubramanian S, Choe SY and Rho GJ. 2007. Effect of donor cell types and passages on preimplantation development and apoptosis in porcine cloned embryos. Asian-Aust. J. Anim. Sci. 20:711-717 https://doi.org/10.5713/ajas.2007.711
  25. Levy RR, Cordonier H, Czyba JC and Guerin JF. 2001. Apoptosis in preimplantation mammalian embryo and genetics. Ital. J. Anat. Embryol. 106:101-108
  26. Li Y, Dai Y, Du WZ, Hao C, Wang H, Wang L, Li R, Liu Y, Wan R and Li N. 2006. Cloned endangered species takin (Budorcas taxicolor) by interspecies nuclear transfer and comparison of the blastocyst development with yak (Bos grunniens) and bovine. Mol. Reprod. Dev. 73:189-195 https://doi.org/10.1002/mrd.20405
  27. Mann MR, Chung YG, Nolen LD, Verona RI, Latham KE and Bartolomei MS. 2003. Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos. Biol. Reprod. 69:902-914 https://doi.org/10.1095/biolreprod.103.017293
  28. McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE and Kind AJ. 2000. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 29: 1066-1069
  29. McGrath J and Solter D. 1984. Inability of mouse blastomere nuclei transferred to enucleated zygotes to support development in vitro. Science 14:1317-1319
  30. Migeon BR, Luo S, Stasiowski BA, Jani M, Axelman J, Van Dyke DL, Weiss L, Jacobs PA, Yang-Feng TL and Wiley JE. 1993. Deficient transcription of XIST from tiny ring X chromosomes in females with severe phenotypes. Proc. Natl. Acad. Sci USA. 90:12025-12029
  31. Moore T and Haig D. 1991. Genomic imprinting in mammalian development: A parental tug-of-war. Trends. Gen. 7:45-49 https://doi.org/10.1016/0168-9525(91)90230-N
  32. Moore T and Reik W. 1996. Genetic conflict in early development: parental imprinting in normal and abnormal growth. Rev. Reprod. 1:73-77 https://doi.org/10.1530/ror.0.0010073
  33. Norris DP, Patel D, Kay GF, Penny GD, Brockdorff N, Sheardown SA and Rastan S. 1994. Evidence that random and imprinted Xist expression is controlled by preemptive methylation. Cell. 77:41-51 https://doi.org/10.1016/0092-8674(94)90233-X
  34. Ogura A, Inoue K, Ogonuki N, Lee J, Kohda T and Ishino F. 2002. Phenotypic effects of somatic cell cloning in the mouse. Cloning Stem. Cells. 4:397-405 https://doi.org/10.1089/153623002321025078
  35. Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H and Perry AC. 2000. Pig cloning by microinjection of fetal fibroblast nuclei. Science 18:1188-1190
  36. Park MR, Cho SK, Lee SY, Choi YJ, Park JY, Kwon DN, Son WJ, Paik SS, Kim T, Han YM and Kim JH. 2005. A rare and often unrecognized cerebromeningitis and hemo-dynamic disorder: A major cause of sudden death in somatic cell cloned piglets. Proteomics. 5:1928-1939 https://doi.org/10.1002/pmic.200401079
  37. Park MR, Cho SK, Park JY, Lee SY, Choi YJ, Kwon DN, Son WJ, Seo HG and Kim JH. 2004. Detection of rare Leydig cell hypoplasia in somatic cell cloned male piglets. Zygote 12:305-313 https://doi.org/10.1017/S0967199404002904
  38. Peippo J, Farazmand A, Kurkilahti M, Markkula M, Basrur PK and King WA. 2002. Sex-chromosome linked gene expression in in-vitro produced bovine embryos. Mol. Hum. Reprod. 8:923-929 https://doi.org/10.1093/molehr/8.10.923
  39. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A and Campbell KH. 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 7:86-90
  40. Ramsoondar JJ, Machaty Z, Costa C, Williams BL, Fodor WL and Bondioli KR. 2003. Production of alpha 1,3-galactosyltransferase- knockout cloned pigs expressing human alpha 1,2-fucosylosyltransferase. Biol. Reprod. 69:437-445 https://doi.org/10.1095/biolreprod.102.014647
  41. Renard JP, Chastant S, Chesne P, Richard C, Marchal J, Cordonnier N, Chavatte P and Vignon X. 1999. Lymphoid hypoplasia and somatic cloning. Lancet. 1:1489-1491
  42. Sasaki H, Allen ND and Surani MA. 1993. DNA methylation and genomic imprinting in mammals. Exp. Suppl. 64:469-486
  43. Shi W, Hoeflich A, Flaswinkel H, Stojkovic M, Wolf E and Zakhartchenko V. 2003. Induction of a senescent-like phenotype does not confer the ability of bovine immortal cells to support the development of nuclear transfer embryos. Biol. Reprod. 69:301-309 https://doi.org/10.1095/biolreprod.102.012112
  44. Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L, Buck S, Murphy K, Lyons L and Westhusin M. 2002. A cat cloned by nuclear transplantation. Nature 415:859 https://doi.org/10.1038/nature723
  45. Stoger R, Kubicka P, Liu CG, Kafri T, Razin A, Cedar H and Barlow DP. 1993. Maternal specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal. Cell. 73:61-71 https://doi.org/10.1016/0092-8674(93)90160-R
  46. Surani MA, Barton SC and Norris ML. 1984. Development of reconstituted mouse eggs suggests imprinting of the genome during gametogenesis. Nature 308:548-550 https://doi.org/10.1038/308548a0
  47. Surani MA, Kothary R, Allen ND, Singh PB, Fundele R, Ferguson- Smith AC and Barton SC. 1990. Genome imprinting and development in the mouse. Dev. Suppl. 89-98
  48. Takagi N and Abe K. 1990. Detrimental effects of two active X chromosomes on early mouse development. Development. 109:189-201
  49. Tilghman SM. 1999. The sins of the fathers and mothers: genomic imprinting in mammalian development. Cell. 22: 185-193
  50. Wakayama T, Perry AC, Zuccotti M, Johnson KR and Yanagimachi R. 1998. Full-term developmentof mice from enucleated oocytes injected with cumulus cell nuclei. Nature 23:369-374 https://doi.org/10.1038/023369a0
  51. Wang ZQ, Fung MR, Barlow DP and Wagner EF. 1994. Regulation of embryonic growth and lysosomal targeting by the imprinted Igf2/Mpr gene. Nature 372:464 https://doi.org/10.1038/372464a0
  52. Wells DN, Misica PM and Tervit HR. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60:996-1005 https://doi.org/10.1095/biolreprod60.4.996
  53. Wilmut I, Beaujean N, De Sousa PA, Dinnyes A, King TJ, Paterson LA, Wells DN, and Young LE. 2002. Somatic cell nuclear transfer. Nature 10:583-586
  54. Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KH. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 27:810-813
  55. Yin XJ, Cho SK, Park MR, Im YJ, Park JJ, Bhak JS, Kwon DN, Jun SH, Kim NH and Kim JH. 2003. Nuclear remodelling and the developmental potential of nuclear transferred porcine oocytes under delayed-activated conditions. Zygote 11:167-174 https://doi.org/10.1017/S096719940300220X
  56. Yoshioka K, Suzuki C, Tanaka A, Anas IM and Iwamura S (2002) Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66:112-129 https://doi.org/10.1095/biolreprod66.1.112
  57. Young LE, Fernandes K, McEvoy TG, Butterwith SC, Gutierrez CG, Carolan C, Broadbent PJ, Robinson JJ, Wilmut I and Sinclair KD. 2001. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat. Genetics. 27:153-154 https://doi.org/10.1038/84769
  58. Zhang S, Kubota C, Yang L, Zhang Y, Page R, O'Neill M, Yang X and Tian C. 2004. Genomic imprinting of H19 in naturally reproduced and cloned cattle. Biol. Reprod. 71: 1540-1544 https://doi.org/10.1095/biolreprod.104.031807