Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Effect of Mineral-induced Alkaline Reduced Water on the DSS-induced Acute Inflammatory Bowel Disease Mouse Model

알칼리환원수 음용이 급성 염증성장질환 생쥐 모델에 미치는 영향

  • Jin, Dan (Department of Immunology and Pathogenic Biology, College of Medicine, Yanbian University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Xufeng, Qi (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • 김단 (연변대학교 기초의학원 면역학과 병원생물학교실) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 최주봉 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실)
  • Published : 2008.06.30
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Abstract

Alkaline reduced water (ARW) has been used for drinking widely in several countries of Asia. The safety and clinical effects of ARW has been reported including anti-oxidative effect and intestinal abnormal fermentation. To confirm the effect of ARW on DSS-induced acute inflammatory bowel disease (IBD) mouse model, we observed the length of total large intestine and the histopathological changes after supplying mineral induced-ARW (MARW) for 2 weeks and oral administration of 4% DSS (dextran sulfate sodium). As the result, the length of total large intestine and the disease scores by macro and microscopical access in the ARWsupplied group showed no significant differences compared with those in the control group. This result suggests that the supply of ARW for 2 weeks exerted no effect on amelioration in the DSS-induced acute IBD model. However, in consideration of the effect of ARW on the improvement of intestinal environment and gastrointestinal disease, this result seems that acute IBD animal model is not suitable or the period of ARW supply is not enough to prove the effect of ARW. The ameliorative effect of ARW on the intestinal abnormal fermentation has been confirmed by some researchers, but the precise mechanism also remain unclear. In conclusion, although MARW had no effect on the DSS-induced acute experimental colitis model, further studies on the verification of the effects of ARW by using other intestinal disease model and by long-term supply of ARW will be required. Also, It needs to clear the mechanism of ARW on the intestinal environment.

알칼리환원수 (Alkaline Reduced Water: ARW)는 아시아를 비롯한 여러 나라에서 음용수로 사용되고 있으며 항산화효과와 장내이상발효 개선효과 등을 중심으로 안전성과 유효성의 검증이 이루어지고 있다. 본 연구에서는 알칼리환원수가 급성 염증성대 장질환 (Inflammatory Bowel Disease: IBD) 동물모델에 미치는 영향을 알아보기 위해, ARW를 2주 동안 급이 시킨 후 4% DDS 로 염증성 대장염을 유발시키고 대장의 길이와 조직병리학적 변화를 관찰하였다. 그 결과 대장의 길이와 육안적, 현미경적 질환점수의 수치가 대조군과 비교하여 유의한 차이를 나타내지 않았다. 이 결과는 DSS로 유도된 급성 염증성장질환 모델에서는 2주 동안의 ARW 급이가 개선효과를 나타내지 않았음을 말해준다. 그러나 ARW가 장내환경을 개선시키고 위장관의 질환을 개선시키는 효과가 있음을 고려해볼 때 급성 IBD 동물모델이 ARW의 효과를 증명 하기에 적합하지 않았거나 MARW의 급이기간이 장내환경을 개선할 만큼 충분히 길지 않았을 가능성을 추측해 볼 수 있다. 알칼리환원수의 장내이상발효 개선효과는 확인되고 있으나 그 기전은 아직까지 구체적으로 확인되어지지 않고 있다. 이번 연구결과에 의하면 인위적으로 유발시킨 급성 염증성 동물모델에서 ARW가 유의한 영향을 미치지 않았지만, 다른 장질환 모델을 이용한 효과 검증과 ARW의 장기 급이에 따른 효과, 그리고 ARW가 장내환경에 미치는 작용기전에 대한 연구가 더 깊이 있게 이루어져야 할 것으로 사료된다.

Keywords

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