Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Fine Structure of the Silk Spigots in the Spider Dolomedes sulfureus (Araneae: Pisauridae)

닷거미과의 황닷거미(Dolomedes sulfureus)의 실크 분비장치의 미세구조

  • Moon, Myung-Jin (Department of Biological Sciences & Institute of Basic Science, Dankook University)
  • 문명진 (단국대학교 첨단과학대학 생명과학과)
  • Published : 2008.06.30
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Abstract

Dolomedes spiders of the family Pisauridae are one of free wandering spiders with semi-aquatic habitation. They do not build web for prey-hunting but build a nursery web for spiderlings. This paper describes the fine structure of the silkspinning spigots of the fishing spider Dolomedes sulfureus revealed by the field emission scanning electron microscope (FESEM). The fishing spider Dolomedes sulfureus possesses only three types of silk glands which connected through the typical spinning tubes on the spinnerets. The silk spigots of this spider were identified as three groups: ampullates, pyriforms and aciniforms. Two pairs of major ampullate glands send secretory ductules to the anterior spinnerets, and another two pairs (or $1{\sim}2$ pairs in males) of minor ampullate glands supply the middle spinnerets. In addition, the pyriform glands feed silk into the anterior spinnerets ($62{\sim}68$ pairs in females and $45{\sim}50$ pairs in males), and the aciniforms send ductules to the middle ($33{\sim}40$ pairs in females and $18{\sim}25$ pairs in males) and the posterior spinnerets ($42{\sim}50$ pairs in females and $24{\sim}28$ pairs in males). Among these, the ampullate one is the most predominate gland in both sexes.

배회성거미류의 일종으로 주로 물가에 서식하는 닷거미과 (Pisauridae)의 황닷거미(Dolomedes sulfureus)를 실험재료로 실크 분비장치의 미세구조를 주사전자현미경(FESEM)으로 관찰하고, 토사관의 미세구조적 특성을 분석하였다. 황닷거미는 먹이를 포획하기 위한 거미줄을 만들지 않지만, 유충을 보호하기 위한 그물을 만드는 종류로 병상선(ampullate glands), 이상선(pyriform glands), 포도상선(aciniform glands) 등, 세 종류의 견사선과 이와 연결된 실젖 표면의 독특한 토사관을 가지고 있었다. 두 쌍의 대 병상선은 앞실젖을 통해, 그리고 또 다른 두 쌍(수컷의 경우 1${\sim}$2쌍)의 소병상선은 가운데실젖을 통해 분비관이 연결되어 있었다. 이상선은 앞실젖 표면의 토사관을 통해(암컷: 62${\sim}$68쌍, 수컷: 45${\sim}$50쌍) 분비되었고, 포도상선은 가운데실젖(암컷: 33${\sim}$40쌍, 수컷:18${\sim}$25쌍)과 뒷실젖(암컷: 42${\sim}$50쌍, 수컷: 24${\sim}$28쌍)의 토사관을 통해 분비됨이 확인되었다. 이들 중, 병상선과 연결된 토사관이 암수 모두에서 가장 현저한 분비 특성을 가진 것으로 관찰되었다.

Keywords

References

  1. Coddington J: The monophyletic origin of the orb web. In: Shear WA, ed, Spiders: Webs, Behavior, and Evolution, pp. 319-363, Stanford University Press, Stanford, CA, USA, 1986
  2. Coddington JA, Levi HW: Systematics and evolution of spiders (Araneae). Ann Rev Ecol Syst 22 : 565-592, 1991 https://doi.org/10.1146/annurev.es.22.110191.003025
  3. Denny M: The physical properties of spider's silk and their role in the design of orb-webs. J Exp Biol 65 : 483-506, 1976
  4. Foelix RF: Biology of Spiders (2nd ed). Oxford University Press, London, 1996
  5. Gertsch WJ: American Spiders (2nd ed). Van Nostrand Reinhold Co., New York, 1979
  6. Gorb SN, Barth FG: Locomotor behavior during prey-capture of a fishing spider, Dolomedes plantarius (Araneae: Araneidae): Galloping and stopping. J Arachnol 22 : 89-93, 1994
  7. Groome JR, Townley MA, de Tschaschell M, Tillinghast EK: Detection and isolation of proctolin-like immunoreactivity in Arachnids: Possible cardioregulatory role for proctolin in the orb-weaving spiders Argiope and Araneus. J Insect Physiol 37 : 9-19, 1991 https://doi.org/10.1016/0022-1910(91)90013-P
  8. Kavanagh EJ, Tillinghast EK: Fibrous and adhesive components of the orb webs of Araneus trifolium and Argiope trifasciata. J Morphol 160 : 17-32, 1979 https://doi.org/10.1002/jmor.1051600104
  9. Kovoor J: Comparative structure and histochemistry of silkproducing organs in Arachnids. In: Nentwig W, ed, Ecophysiology of Spiders, pp. 159-186, Springer-Verlag, Berlin, 1987
  10. Moon MJ: Fine structural analysis of the silk producing apparatus in wolf spider, Pardosa astrigera (Araneae: Lycosidae). Korean J Entomol 28 : 201-211, 1998a
  11. Moon MJ: Fine structure of the silk producing apparatus in the garden spider, Argiope bruennichi. Korean J Entomol 28 : 345-354, 1998b
  12. Moon MJ: Microstructure of the silk spinning nozzles in the lynx spider, Oxyopes licenti (Araneae: Oxyopidae). Integrative Biosciences 10 : 85-91, 2006
  13. Moon MJ, An JS: Spinneret microstructure of silk spinning apparatus in the crab spider, Misumenops tricuspidatus (Araneae: Thomisidae). Entomol Res 35 : 67-74, 2005 https://doi.org/10.1111/j.1748-5967.2005.tb00138.x
  14. Moon MJ, An JS: Microstructure of silk spigot of the green crab spider, Oxytate striatipes (Araneae: Thomisidae). Entomol Res 36 : 133-138, 2006 https://doi.org/10.1111/j.1748-5967.2006.00023.x
  15. Moon MJ, Kim TH: Microstructural analysis of the capture thread spinning apparatus in orb web spiders. Entomol Res 35 : 133-140, 2005 https://doi.org/10.1111/j.1748-5967.2005.tb00149.x
  16. Moon MJ, Kim WK: Fine structural study on the capture threads -producing organs in Nephila clavata L. Koch (Araneae: Araneidae). (I) Aggregate glands. Kor J Zool 32 : 211-220, 1989
  17. Moon MJ, Tillinghast EK: Silk production after mechanical pulling stimulation in the ampullate silk glands of the barn spider, Araneus cavaticus. Entomol Res 34 : 123-130, 2004 https://doi.org/10.1111/j.1748-5967.2004.tb00101.x
  18. Nentwig W, Heimer S: Ecological aspects of spider webs. In: Nentwig W, ed, Ecophysiology of Spiders, pp. 211-225, Springer-Verlag, Berlin, 1987
  19. Park JG, Moon MJ: Fine structure of the spinning apparatus in the funnel-web spider, Agelena limbata. Korean J Entomol 32 : 223 -232, 2002 https://doi.org/10.1111/j.1748-5967.2002.tb00033.x
  20. Peters HM: Fine structure and function of capture threads. In: Nentwig W, ed, Ecophysiology of Spiders, pp. 187-202, Springer-Verlag, Berlin, 1987
  21. Peters HM, Kovoor J: The silk-producing system of Linyphia triangularis (Araneae: Linyphiidae) and some comparisons with Araneidae: Structure, histochemistry and function. Zoomorphology 111 : 1-17, 1991
  22. Shear WA: Untangling the evolution of the web. Amer Sci 82 : 256-266, 1994
  23. Suter RB, Gruenwald J: Predator avoidance on the water surface? Kinematics and efficacy of vertical jumping by Dolomedes (Araneae, Pisauridae). J Arachnol 28 : 201-210, 2000 https://doi.org/10.1636/0161-8202(2000)028[0201:PAOTWS]2.0.CO;2
  24. Suter RB, Rosenberg O, Loeb S, Wildman H, Long JH Jr: Locomotion on the water surface: Propulsive mechanisms of the fisher spider Dolomedes triton. J Exp Biol 200 : 2523-2538, 1997
  25. Tillinghast EK, Christenson T: Observations on the chemical composition of the web of Nephila clavipes (Araneae: Araneidae). J Arachnol 12 : 69-74, 1984
  26. Tillinghast EK, Townley MA: The independent regulation of protein synthesis in the major ampullate glands of Araneus cavaticus Keyserling. J Insect Physiol 32 : 117-123, 1986 https://doi.org/10.1016/0022-1910(86)90130-7
  27. Townley MA, Horner NV, Cherim NA, Tugmon CR, Tillinghast EK: Selected aspects of spinning apparatus development in Araneus cavaticus (Araneae: Araneidae). J Morphol 208 : 175-191, 1991