Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antiwrinkle Effects of Mugwort (Artemisia vulgaris) Extracts on UVB-Irradiated Hairless Mouse Skin

자외선 조사 무모쥐 피부조직에 도포한 애엽(Mugwort) 추출물의 주름개선 효과

  • Park, Si-Hyang (Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Hong, Yu-Mi (Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Yeung-Joon (Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Jin-Ho (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Kim, Byung-Kwan (Dept. of Chemical Engineering, Changwon National University)
  • 박시향 (경상대학교 해양과학대학 해양생명과학부/해양산업연구소) ;
  • 홍유미 (경상대학교 해양과학대학 해양생명과학부/해양산업연구소) ;
  • 최영준 (경상대학교 해양과학대학 해양생명과학부/해양산업연구소) ;
  • 최진호 (부경대학교 식품생명공학부) ;
  • 김병관 (창원대학교 공과대학 화공시스템공학과)
  • Published : 2008.09.30
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Abstract

This study was to investigate antiwrinkle effect of mugwort (Artemisia vulgaris) methanol extract in hairless mouse skin induced by UVB-irradiation. Hairless mouse were topically treated with the basic lotion alone (control), ascorbic acid (AA-0.5%, AA-1.0%, AA-2.0%, and AA-5.0%) and mugwort extract (ME-0.5%, ME-1.0%, ME-2.0%, and ME-5.0%) dissolved in a basic lotion. After topical treatment of 30 minutes, the animals were irradiated with increasing doses of UVB radiation ($60{\sim}100\;mJ/cm^2$) for 4 weeks. In our experimental condition, skin thickness of hairless mouse was significantly decreased ($12.5{\sim}21.4%$) in all ME groups compared with control group. Ra value, that is surface roughness parameter induced by skin wrinkling, was significantly decreased ($23.7{\sim}31.1%$) in ME-1.0%, 2.0% and 5.0% group compared with control group. Furthermore, Rq, Rz and Rt value were significantly decreased to $11.2{\sim}21.2%$, $19.8%{\sim}24.5%$, and $14.2%{\sim}22.7%$, respectively. Wrinkle formation of ascorbic acid treatment group as reference group was inhibited, but its effect was less than ME treatment. Matrix metalloproteinase-1 activity was significantly inhibited ($19.7{\sim}22.6%$) compared with control group and collagen content was significantly increased (about 10%) when compared with control group. These results indicate that ME could protect skin aging and wrinkle formation in hairless mouse from photo-irradiation.

무모쥐의 등에 애엽 추출물과 아스코르브산 함유 스킨로션을 도포하고 자외선을 조사하여 애엽 추출물과 아스코르브산의 주름 개선 효과를 알아보았다. 애엽 추출물 도포군의 피부조직 두께는 대조군에 비해 $12.5{\sim}21.4%$의 유의적인 감소 효과를 보였다. 표면 거칠기 측정기에 의한 피부 주름의 형성 정도를 측정하는 지표인 Ra값은 ME-1.0, ME-2.0과 ME-5.0 도포군에서 $23.7{\sim}31.1%$로 유의적으로 감소하였고 (p<0.01), Rq치는 ME-1.0, ME-2.0와 ME-5.0에서 $11.2{\sim}21.2%$, Rz의 측정치에서는 ME-1.0(19.8%), ME-2.0(22.1%), ME-5.0(24.5%) 그룹에서 대조군에 비해 크게 감소하였다. Rt치는 ME-1.0, ME-2.0와 ME-5.0 그룹에서 농도 증가에 따라 14.2%에서 22.7%의 유의적인 감소효과가 있었다. 아스코르브산 도포그룹은 애엽과 비슷한 감소효과를 보여주었지만, 애엽의 주름 개선효과에는 미치지 못하였다. MMP-1 활성은 애엽 메탄올 추출물 그룹에서 $19.3%{\sim}22.6%$까지 감소하였고, 콜라겐의 생성은 ME-2.0과 ME-5.0그룹에서 10% 정도의 유의적인 증가 효과가 있었다. 이처럼 애엽 메탄올 추출물은 피부의 광손상에 대한 피부 보호 및 피부의 주름 개선효과가 탁월하고, 또한 강력한 항산화제인 아스코르브산보다 그 효과가 우수하여 피부노화 현상과 주름 개선을 위한 기능성 화장품 소재로 이용가치가 있을 것으로 여겨진다.

Keywords

References

  1. Lee JN, Kim SW, Yoo YK, Lee GT, Lee KK. 2006. Anti-wrinkle effect of Morinda citrifolia (Noni) extracts. J Soc Cosmet Scientists Korea 32: 227-231
  2. Jean LB. 1995. Aging skin. Am J Med 8: S99-S103
  3. Laure RG, Fisher J. 2002. UV-light-induced signal cascades and skin aging. Ageing Res Rev 1: 705-720 https://doi.org/10.1016/S1568-1637(02)00024-7
  4. Varani J, Perone P, Fligiel G, Fisher GJ, Voorhees JJ. 2002. Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis. J Invest Dermatol 119: 122-129 https://doi.org/10.1046/j.1523-1747.2002.01810.x
  5. Chung JH, Seo JY, Choi HR, Lee MK, Youn CS, Rhie GE, Cho KH, Kim KH, Park KC, Eun HC. 2001. Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol 117: 1218-1224 https://doi.org/10.1046/j.0022-202x.2001.01544.x
  6. Kang SJ. 1999. Skin photoaging in reconstituted skin culture models. J Soc Cosmet Scientists Korea 25: 59-74
  7. Fisher GJ, Choi HC, Bata-Csorgo Y, Shao S, Datta ZQ, Wang SK, Voorhees JJ. 2001. Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J Invest Dermatol 117: 219-226 https://doi.org/10.1046/j.0022-202x.2001.01432.x
  8. Simon C, Ilona K, Tramposch KM. 1992. Effects of all-trans retinoic acid on UVB-irradiated and non-irradiated hairless mouse skin. J Invest Dermatol 98: 248-260 https://doi.org/10.1111/1523-1747.ep12462236
  9. Elaine S, Frederick AC, James AM, Lorraine HK. 1991. Topical all-trans retinoic acid stimulates collagen synthesis in vivo. J Invest Dermatol 96: 975-978 https://doi.org/10.1111/1523-1747.ep12476385
  10. Shin MH, Rhie GE, Park CH, Kim KH, Cho KH, Eun HH, Chung JH. 2005. Effect of DHEA on skin collagen metabolism. J Invest Dermatol 124: 315-323 https://doi.org/10.1111/j.0022-202X.2004.23588.x
  11. Kim SW, Jeong JH, Jo BK. 2004. Anti-wrinkle effect by ginsenoside Rg3 derived from ginseng. J Soc Cosmet Scientists Korea 30: 221-225
  12. Francesco B, Maria L, Lucia M, Claudio P, Antonio T, Domenico T, Francesco C, Antonella S. 1996. Flavonoids as potential protective agents against photo-oxidative skin damage. Int J Pharm 145: 87-94 https://doi.org/10.1016/S0378-5173(96)04728-X
  13. Choi BB, Lee HJ, Bang SK. 2004. Studies on the amino acid, sugar analysis and antioxidative effect of extracts from Artemisia sp. Korean J Food Nutr 17: 86-91
  14. Choi HC, Oh CH. 1997. Evaluation of skin furrows in the ageing process using an image analysis system. Korean J Dermatol 35: 292-302
  15. Lee MY, Kim EJ, Lee HK, Seu YK, Lee MS, Koh JS. 2004. Evaluation of age-dependent crow's feet in Korean women. J Soc Cosmet Scientists Korea 30: 85-91
  16. Florian K, Michael WD, Michael D, Lars DK, Hermann JV, Siegfried B. 1995. Direct profilometry of the skin: its reproducibility and variability. J Eur Acad Dermatol Venerol 5: 15-23 https://doi.org/10.1111/j.1468-3083.1995.tb00428.x
  17. Kim YP, Lee SC. 1987. Superoxide dismutase activites in the human skin. The biological role of reactive oxygen species in skin. University of Tokyo Press, Tokyo. p 225-320
  18. Yoshinori T, Yukiko Y, Michio K. 1995. The relationship between age- related changes in the physical properties and development of wrinkles in human facial skin. J Soc Cosmet Scientists Korea 46: 163-173
  19. Gail J. 2002. Molecular mechanism of skin ageing. Mech Ageing Dev 123: 801-810 https://doi.org/10.1016/S0047-6374(01)00425-0
  20. Suzanne EGF, James V, Subhash CD, Sewon K, Gary JF, John J, Voorhees. 2003. Collagen degradation in aged/photodamaged sin in vivo and after exposure to matrix metalloproteinase-1 in vitro. J Invest Dermatol 120: 842-848 https://doi.org/10.1046/j.1523-1747.2003.12148.x
  21. Varani J, Warner RL, Gharaee KM, Phan SH, Kang S, Chung JH, Wang Z, Datta SC, Fisher GJ, Voorhees JJ. 2000. Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin. J Invest Dermatol 114: 480-486 https://doi.org/10.1046/j.1523-1747.2000.00902.x
  22. Karna E, Miltyk W, Wolczynski S, Palka JA. 2001. The potential mechanism for glutamine-induced collagen biosynthesis in cultured human skin fibroblasts. Comp Biochem Physiol 130: 23-32 https://doi.org/10.1016/S1096-4959(01)00400-6
  23. Park SH, Cho DM, Choi BD, Choi YJ, Choi JH. 2008. Antioxidative effects of skinned mugwort (Artemisia vulgris L.) extracts on UV-irradiated hairless mouse skin. J Korean Soc Food Sci Nutr 37: 20-26 https://doi.org/10.3746/jkfn.2008.37.1.20
  24. Kim KD. 2004. Research and application for natural extract that contain ultraviolet rays absorbent ingredient. J Soc Cosmet Scientists Korea 30: 117-122
  25. Ryu SN, Kang SS, Kim JS, Ku BI. 2004. Quantitative analysis of eupatilin and jaceosidin in Artemisia herba. Korean J Crop Sci 49: 452-456
  26. Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol 27: 978-984

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