Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Experimental Studies on the Hair Growth Activity of Mixed Extracts of Mylabris Phalerata Pall. and Drynariae Rhizoma in Spontaneous Alopecia Model and Normal C57BL/6N Mice

자발적인 원형탈모 생쥐(C57BL/6N)와 정상적인 생쥐에서 반모(斑毛)와 골쇄보(骨碎補) 추출 혼합물의 발모(發毛)효과에 대한 실험적 연구

  • Lee, Moon-Won (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jo, Han-Young (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Tae-Heon (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Nam-Seok (Department of Food & Biotechnology, Woosuk University) ;
  • Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 이문원 (우석대학교 한의과대학 해부학교실) ;
  • 조한영 (우석대학교 한의과대학 해부학교실) ;
  • 김태헌 (우석대학교 한의과대학 해부학교실) ;
  • 김남석 (우석대학교 식품생명공학과) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Published : 2008.08.25
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Abstract

This experiment investigated the effect of mixed extracts obtained from Mylabris phalerata Pall. and Drynariae Rhizoma on hair growth activity of the normal and spontaneous alopecia areata model of C57BL/6N mice for 16 days. First, we examined morphological regrowth of hair in normal and spontaneous alopecia model of C57BL/6N mice. Second, we examined immunoreactive density of vascular endothelial growth factor(VEGF), c-kit and protein kinase $C-{\alpha}(PKC-{\alpha})$ in skin of normal C57BL/6N mice by immunohistochemical methods. Third, we investigated expression of $TGF-{\beta}$, prolactin and placenta lactogen after topical application of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma to skin by RT-PCR. The results were as follows: Hair growth effect from middle and high concentration of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma was observed in 80% of normal mice in whose hair had been clipped in 15th days. Hair growth effect of all concentrations of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma was observed in 100% of spontaneous alopecia model of C57BL/6N mice in 15th days. Immunoreactive density of VEGF, c-kit and $PKC-{\alpha}$ in skin of all concentrations of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma were strongly stained in epidermis, bulge, secondary hair germ cells and cutaneous trunci m. compare to control group in 10th day. In experimental III group, Immunoreactive density of VEGF, c-kit and $PKC-{\alpha}$ in skin were strongly stained in inner and outer root sheath of skin. The treatment of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma increased the expression of $TGF-{\beta}$, placenta lactogen and prolactin in the skin of normal C57BL/6N mice compared to control group. These experiments suggest that mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma may stimulate the topical hair growth activity and it can be useful for treatment of alopecia areata.

Keywords

References

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