Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Development of a Simultaneous Analysis Method for Disapproved Coloring Agents in Foods Using HPLC

HPLC를 이용한 식품중 허용 외 색소의 동시분석에 관한 연구

  • Kim, Cheon-Hoe (Korea Health Supplement Institute) ;
  • Lee, Min-Jae (Korea Health Supplement Institute) ;
  • Kim, Keoung-Ok (Korea Health Supplement Institute) ;
  • Lee, Hyoun-Young (Korea Health Supplement Institute) ;
  • Yang, Joo-Hong (Korea Health Supplement Institute) ;
  • Heo, Seok (Hazard Substances Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food & Drug Administration) ;
  • Park, Jong-Seok (Hazard Substances Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food & Drug Administration) ;
  • Jang, Yeong-Mi (Hazard Substances Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food & Drug Administration) ;
  • Kim, Hee-Yun (Hazard Substances Analysis Division, Center for Food and Drug Analysis, Gyeongin Regional Korea Food & Drug Administration)
  • 김천회 (한국기능식품연구원) ;
  • 이민재 (한국기능식품연구원) ;
  • 김경옥 (한국기능식품연구원) ;
  • 이현영 (한국기능식품연구원) ;
  • 양주홍 (한국기능식품연구원) ;
  • 허석 (경인지방식품의약품안전청 유해물질분석과) ;
  • 박종석 (경인지방식품의약품안전청 유해물질분석과) ;
  • 장영미 (경인지방식품의약품안전청 유해물질분석과) ;
  • 김희연 (경인지방식품의약품안전청 유해물질분석과)
  • Published : 2008.08.01
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Abstract

This study developed the simultaneous analysis method for efficient safety controls of import food of Orange II, Rhodamine B, Para Red, and Sudan dye I-IV among disapproved coloring agents that use is prohibited in foods. The analysis method was developed according to the sample pre-treatment and HPLC conditions, and a documentary survey was used to establish the detection limit of the method, followed by effectiveness verification and recovery percentage examinations. Recovery percentage examination for 26 products resulted Orange II displayed recovery percentage of 96.46-121.26%, Rhodamine B displayed recovery percentage of 70.86-106.53%, Para Red displayed recovery percentage of 97.00-116.86%, Sudan I displayed recovery percentage of 92.93-112.44%, Sudan II displayed recovery percentage of 96.63-115.10%, Sudan III displayed recovery percentage of 92.21-114.73%, Sudan IV displayed recovery percentage of 93.22-122.91%. Correlation coefficient of gradient of this analysis method exhibited more than 0.999, RSD exhibited fewer than 2 as 0.8-1.39%, exactitude exhibited more than 90%. At this time, detection limit and fixed quantity limit decided by each 0.1 mg/L, 0.3 mg/L.

식품에 사용이 금지된 허용 외 색소 중 Orange II, Rhodamine B, Para red, Sudan I-IV 색소의 효율적인 수입식품 안전관리와 수용성 착색료 및 지용성 착색료로 이원화되어 있는 허용 외 착색료의 신속하고 정확한 분석을 위하여 동시 분석법을 개발하고자 하였다. 문헌조사를 바탕으로 분석을 위한 컬럼, 이동상 조건, 분석파장, 전처리 조건 등을 검토하여 분석법을 개발하였으며, 개발된 분석법의 검출한계 설정, 유효성 검증 및 회수율을 검토하였다. 26가지 제품에 대한 회수율 검토결과 Orange II는 96.46-121.26%, Rhodamine B는 70.86-106.53%, Para red는 97.00-116.86%, Sudan I은 92.93-112.44%, Sudan II는 96.63-115.10%, Sudan III는 92.21-114.73%, Sudan IV는 93.22-122.91%의 회수율을 나타냈다. 본 분석법 검량선의 상관계수는 0.999 이상을 보였으며 정밀성의 RSD는 0.8-1.39%로서 모두 2 이하의 값을 보였으며, 정확성은 90% 이상을 나타냈다. 이때, 검출한계 및 정량한계는 각각 0.1 mg/L, 0.3 mg/L로 결정하였다.

Keywords

References

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