Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Cholesterol Diet and Exercise on Plasma and Liver Lipids, Platelet Aggregation and Erythrocyte Na Efflux in Rats

콜레스테롤 식이와 운동이 흰쥐의 체내 지질수준, 혈소판 응집 및 적혈구막 Na 유출에 미치는 영향

  • Kang, Min-Sook (Department of Food & Nutrition Science, College of Natural Sciences, Cheju National University) ;
  • Kim, Young-Pyo (Division of Exercise & Sports Science, Cheju National University) ;
  • Kang, Young-Hee (Division of Life Sciences and Institute of Environment and Life Science, Hallym University) ;
  • Kang, Jung-Sook (Department of Food & Nutrition Science, College of Natural Sciences, Cheju National University)
  • 강민숙 (제주대학교 자연과학대학 식품영양학과) ;
  • 김영표 (제주대학교 체육학부) ;
  • 강영희 (한림대학교 식품영양학과) ;
  • 강정숙 (제주대학교 자연과학대학 식품영양학과)
  • Published : 2008.07.30
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Abstract

Present study was conducted to investigate the effects of exercise and cholesterol diet on plasma and liver lipids, platelet aggregation, erythrocyte Na efflux and liver index such as GOT and GPT using Sprague Dawley rats. Forty rats were divided into four groups and fed control or 0.5% cholesterol diet with and without treadmill for six weeks. The final body weight of group fed cholesterol diet with exercise was somewhat decreased compared with group fed cholesterol diet without exercise. L.W/B.W ratio was significantly increased in groups fed cholesterol diet (p < 0.01), but exercise tended to decrease this ratio. Plasma total cholesterol was significantly increased and HDL-cholesterol was decreased in groups fed cholesterol diet (p < 0.01). Plasma triglyceride was significantly decreased in groups fed cholesterol diet compared with groups fed control diet (p < 0.01). Plasma triglyceride of groups with exercise was significantly decreased compared with their non exercising counterparts regardless diet (p < 0.05). Liver total cholesterol and triglyceride was significantly increased in groups fed cholesterol diet (p < 0.01), but exercise did not affect on these levels. Na-K ATPase was somewhat decreased in groups fed cholesterol diet, and exercise tended to recover the reduced Na-K ATPase. Na passive transport was significantly decreased in group fed control diet without exercise and significantly increased in group fed cholesterol diet with exercise, there were significant differences between groups (p < 0.05). There were no differences in total Na efflux and intracellular Na among groups, and total Na efflux was not correlated with intracellular Na. Hematocrit was significantly lower (p < 0.05) in group fed cholesterol diet without exercise compared with other groups. Platelet aggregation in the initial slope and the maximum was increased in groups fed cholesterol diet, but not statistically significant. Exercise especially increased the initial slope of aggregation. Plasma GOT and GPT was significantly increased in groups fed cholesterol diet (p < 0.01), and exercise in group fed cholesterol diet significantly decreased both GOT and GPT compared with the non exercising counterpart (p < 0.01). This study showed that cholesterol diet increases plasma and liver lipids and GOT and GPT, and exercise improves plasma and liver lipid profile and liver index of GOT and GPT preventing fatty liver.

본 실험은 콜레스테롤식이와 운동수행이란 두 조건을 심순환기 질환의 관련지표로서 혈장 및 간의 지질, 적혈구막의 Na efflux 그리고 혈소판 응집성에 어떠한 영향을 미치는지 조사하였다. 운동에 의해서 체중이 다소 감소하였지만 그렇게 크지는 않았다. 라드를 주 지방원으로 사용한 식이에 0.5% 콜레스테롤첨가는 혈중 콜레스테롤을 2배 이상, 간 콜레스테롤을 8배 이상 증가시켰고 HDL-콜레스테롤을 절반 이하로 떨어뜨렸는데, 특히 운동효과로 예상했던 HDL-콜레스테롤증가는 대조군식이나 콜레스테롤식이에서 나타나지 않았다. 혈중 중성지방은 콜레스테롤 식이군에서 오히려 감소하였고 간의 경우 지방 축척으로 인해 간의 크 기뿐만 아니라 간의 중성지방은 현저히 증가되었다. 체액량 조절 및 혈압과 관계에서 조사된 적혈구의 Na efflux는 Na-K ATPase가 콜레스테롤 식이에 의해 그 활성을 감소되었고 운동이 이를 다소 회복시키는 경향을 보였다. 혈압과의 상관성이 높다고 알려진 Na passive leak은 콜레스테롤과 운동이 각각 독립적으로 증가시키는 요인으로 작용하여 콜레스테롤-운동군이 대조군식이-비운동군에 비해 현저히 증가된 것으로 나타났다. 총 Na efflux는 실험군 간에 큰 차이가 없었고 intracellular Na와의 상관성도 보이질 않았다. 혈소판응집성에 있어서 콜레스테롤식이가 혈소판 응집을 증가시키고 특히 운동이 초기응집을 증가시키는 경향을 보였다. 혈장 GOT 및 GPT수치는 콜레스테롤식이로 인해 현저히 증가됨으로서 간의 병리적 상태를 잘 반영하였고 운동에 의해 감소됨으로서 운동수행이 술이나 고지방식으로 인한 지방간을 호전시켜 줄 수 있으리라 기대할 수 있다. 고지혈증이나 고혈압 환자에 있어서 비약물적 요법으로 식이나 운동을 통한 치료나 예방의 가능성을 규명하기 위한 실험의 결과로서 특히 운동에 대해 일관되고 가시적인 효과가 미흡한 것은 6주간의 짧은 실험기간을 생각할 수 있는데 혈청 지질, Na flux 및 체액, 혈소판 응집성 등 심순환기 질환의 예방에 도움이 되는 운동의 형태나 강도 혹은 지속 기간에 대한 더 많은 연구가 필요하리라 생각된다.

Keywords

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