Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Production of Salidroside in Rhodiloa sachalinensis A. Bor Callus by the Elicitation and Precursor

참돌꽃 (Rhodiola sachalinensis A. Bor) 캘러스에서 elicitor와 전구체에 의한 Salidroside 생산성의 변화

  • Lee, Jae-Seung (Department Education of Biology, Sunchon University Graduate School Of Education) ;
  • Kim, Min-Young (Jeollanamdo Agricultural Research and Extension Services) ;
  • Kim, Jae-Heun (Korea Forest Research Institute) ;
  • Nam, Jong-Hyun (Glami Co., LTD) ;
  • Lee, Hyeon-Young (College of Bioscience & Biotechnology, Kangwon National. University) ;
  • Hwang, Baik (Deppartment. of Biology, Chonnam National University)
  • Published : 2008.08.30
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Abstract

The effect of elicitor and precursor on salidroside production from Rhodiola sachalinensis A.Bor callus cultures was investigated. Callus cultures were treated with yeast extract, soft-ferrite ceramics powder, methyl jasmonate, ascorbic acid, jasmonic acid and $CuCl_2$/$CdCl_2$ as an elicitor. When callus cultures were treated with $0.2g/\ell$ of yeast extract, salidroside production from callus treated with yeast extract is 3.45 times higher than that of the controlled group. Among of them, callus cultures treated with yeast extract produced the highest salidroside. Callus cultures were treated with L-phenylalanine and L-tyrosine as a precursor for 4 days. The result of salidroside content analysis showed that all feeding of precursors not affected salidroside production from callus cultures. In case of L-tyrosine fed into callus cultures, both callus growth and salidroside production decreased at all concentrations.

참돌꽃 캘러스로부터 elicitor와 전구체가 salidroside 생산에 미치는 영향을 조사하였다. Elicitor로서 효모추출물, 연자성 세라믹, methyl jasmonate, jasmonic acid, ascorbic acid, 및 중금속 ($CuCl_2$/$CdCl_2$)을 캘러스 배양에 처리하였다. 효모추출물 $0.2g/\ell$농도로 처리한 결과 처리하지 않은 대조구에 보다 3.45배 증가시켰다. 사용된 elicitor 중 효모추출물이 가장 높은 salidroside 생산을 보여 가장 적합한 elicitor로 사료된다. 전구체로서 L-phenylalanine과 L-tyrosine을 배지에 첨가하여 4일 간 배양 처리하였다. Salidroside 함량분석 결과 캘러스로부터 전구체들은 유용물질 생합성에 영향을 주지는 않았다. 캘러스 배양에 첨가 처리된 L-tyrosine의 모든 농도의 경우에는 캘러스 생장뿐만 아니라 salidroside 생산을 감소시켰다.

Keywords

References

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