Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidative Activity and Nitrite Scavenging Ability of Ethanol Extract from Nelumbo nucifera Leaves

연잎 에탄올 추출물의 항산화 효과 및 아질산염 소거능

  • Lim, Jin-A (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Lee, Eun-Sook (Department of Herbal Skin Care, Daegu Haany University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
  • 임진아 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이은숙 (대구한의대학교 한방피부미용학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2008.06.25
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Abstract

To confirm possibility of Nelumbo nucifera leaves as biofunctional material, we investigated the antioxidant activity and nitrite scavenging ability of ethanol extract from Nelumbo nucifera leaves. Nelumbo nucifera leaves were extracted with ethanol and concentrated under vacuum using rotary evaporator. Then, antioxidative activity and nitrite scavenging ability of the extract were examined in vitro. Electron -donating ability of the extract at RC50 was 90.19 ${\mu}g/mL$. After addition of 0.96 mg/mL, autooxidation of pyrogallol decreased to 66.19% by superoxide dismutase-like activity. In antioxidative activity of the extract against linoleic acid during incubation times of 24, 48, and 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased to 72.53%, 82.00%, 84.69% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and the value revealed to be $282.84\;{\pm}\;9.03$ GAE ${\mu}g/mg$ of the extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 45.55% by addition of 0.2 mg/mL. These results suggest that ethanol extract from N. nucifera leaves can be used as bioactive and functional material.

Keywords

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