Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Control of Acetate Production Rate in Escherichia coli by Regulating Expression of Single-Copy pta Using $lacI^Q$ in Multicopy Plasmid

  • Lee, Sun-Gu (Department of Chemical and Biochemical Engineering, Pusan National University) ;
  • Liao, James C (Department of Chemical and Biomolecular Engineering, University of California Los Angeles)
  • Published : 2008.02.29
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Abstract

A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosphate was constructed by integrating pta, a gene encoding phosphotransacetylase, under a tac promoter into the chromosome of E. coli with a pta-negative background and transforming a multicopy plasmid containing the $lacI^Q$ gene into the strain. The production rate of acetate was shown to be tightly controlled when varying the concentration of the inducer (IPTG) in he model system.

Keywords

References

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