Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Selective Production of Epothilone B by Heterologous Expression of Propionyl-CoA Synthetase in Sorangium cellulosum

  • Han, Se-Jong (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Park, Sang-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Kim, Byung-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Sim, Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University)
  • Published : 2008.01.31
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Abstract

The metabolic engineering of epothilones, as secondary metabolites, was investigated using Sorangium cellulosum to achieve the selective production of epothilone B, a potent anticancer agent. Thus, the propionyl-CoA synthetase gene (prpE) from Ralstonia solanacearum was heterologously expressed in S. cellulosum to increase the production of epothilone B. Propionyl-CoA synthetase converts propionate into propionyl-CoA, a potent precursor of epothilone B. The recombinant S. celluloslim containing the prpE gene exhibited a significant increase in the resolution of epothilones B/A, with an epothilone B to A ratio of 127 to 1, which was 100 times higher than that of the wild-type cells, demonstrating its potential use for the selective production of epothilone B.

Keywords

References

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