Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Extraction and Analysis of Astaxanthin from Haematococcus pluvialis Using Sonication

초음파처리를 이용한 Haematococcus pluvialis로부터의 아스타잔틴의 추출 및 분석

  • Kim, So-Young (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • Cho, Eun-Ah (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • Yoo, Ji-Min (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • In, Man-Jin (Dept. of Human Nutrition and Food Science, Chungwoon University) ;
  • Chae, Hee-Jeong (Dept. of Food and Biotechnology, Center for Food Function and Safety, Hoseo University)
  • 김소영 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 조은아 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 유지민 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 인만진 (청운대학교 식품영양학과) ;
  • 채희정 (호서대학교 식품생물공학과, 식품기능안전연구센터)
  • Published : 2008.10.31
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Abstract

The extraction and quantitative analysis conditions for astaxanthin from Haematococcus pluvialis, and the structural characteristics of H. pluvialis extract, H. pluvialis hydrolysate and synthetic astaxanthin were investigated using UV/visible and FT-IR spectrometers. Astaxanthin was dissolved in methanol, and then treated to enhance the solubility by sonication for 45 min. With sonication pretreatment, the solubility of astaxanthin increased up to 1.5 times compared to that without sonication. The extracts were hydrolyzed by cholesterol esterase for the analysis of H. pluvialis extract containing astaxanthin ester. A HPLC method using reverse phase C18 column with methanol-water (95:5, v/v) as mobile phase was developed to analyze astaxanthin. After hydrolysis, the absorption spectrum of H. pluvialis hydrolysat was changed to similar pattern to synthetic astaxanthin, confirming the extraction and analysis condition of astaxanthin from H. pluvialis.

본 연구에서는 헤마토코커스 플루비알리스(H. pluvialis)의 파쇄세포(cracked cell)로부터 추출된 아스타잔틴의 HPLC 분석방법을 확립하고, UV/visible 및 FT-IR 분광분석법을 이용하여 헤마토코커스 추출물과 합성 아스타잔틴의 구조적 특성을 조사하였다. 아스타잔틴을 메탄올 용매에 녹이고, 45분 정도 초음파로 처리할 경우 교반처리하는 경우 보다 1.5배 정도 용해율이 높아지는 것을 확인하였다. 에스터 형태로 존재하는 추출물 중의 아스타잔틴의 분석을 위해 cholesterol esterase를 이용해 헤마토코커스 추출물을 가수분해처리를 하여 유리형으로 전환시킨 후 HPLC 분석을 수행하였다. 아스타잔틴의 함량을 분석하기 위해 역상칼럼(C18)과 UV/visible 검출기를 사용하였고, 이동상은 메탄올과 물(95:5)의 혼합용매를 사용하여 분석하였다. 효소처리 후, 헤마토코커스 추출물의 흡수스펙트럼이 합성 아스타잔틴과 유사한 패턴으로 변화하는 것을 확인함으로써 헤마토코커스로부터의 아스타잔틴의 추출 및 분석조건을 확립하였다.

Keywords

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