Effect of Heating Temperature on Antimicrobial Activities of Garlic Juice

가열온도가 마늘즙의 항균활성에 미치는 영향

  • Ko, Myung-Soo (Department of Food Science and Biotechnology, Dongnam Health College) ;
  • Yang, Jong-Beom (Department of Food Science and Biotechnology, Dongnam Health College)
  • 고명수 (동남보건대학 식품생명과학과) ;
  • 양종범 (동남보건대학 식품생명과학과)
  • Published : 2008.08.30

Abstract

The antimicrobial activities of raw garlic and garlic juice heated for 30 min at $65^{\circ}C,\;80^{\circ}C,\;and\;95^{\circ}C$ were investigated. Activities against food poisoning bacteria were measured. Raw garlic and garlic juice heated at $65^{\circ}C$ showed strong antimicrobial activities, but the antimicrobial activity of garlic juice heated at $80^{\circ}C$ was much less, and no antimicrobial activity was seen in garlic juice heated at $95^{\circ}C$. Raw garlic and garlic juice heated at $65^{\circ}C$ completely inhibited the growth of Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Salmonella typhymurium, and Salmonella enteritidis at levels below 0.2%(w/v) of garlic solids. The inhibitory effects of garlic juice heated at $80^{\circ}C$ were much lower, against all bacteria tested.

본 연구에서는 가열온도를 달리하여 마늘즙을 열처리한 후 생마늘즙과 열처리 마늘즙의 식중독세균에 대한 항균활성을 조사하였다. 생마늘즙과 $65^{\circ}C$에서 30분간 열처리된 마늘즙은 공시된 모든 균주에 대해 강한 항균활성을 나타내었으나, 가열온도가 높을수록 항균활성이 낮아져 $80^{\circ}C$에서 30분간 열처리된 마늘즙은 항균활성이 크게 저하되었고, $95^{\circ}C$에서 30분간 열처리된 마늘즙은 모든 균주에 대해 항균활성을 보이지 않았다. 생마늘즙은 Bacillus subtilis, Bacillus cereus, Staphylococcus aureus 및 Vibrio parahaemolyticus에 대해 고형분 함량 0.1%의 농도에서, Salmonella typhymurium과 Salmonella enteritidis에 대해서는 고형분 함량 0.2%의 농도에서 생육을 완전히 억제시켜 강한 생육저해효과를 나타내었고, Listeria monocytogenes와 Escherichia coli에 대해서는 고형분 함량 0.8%의 농도에서 생육을 완전히 억제시켜 이 균들은 다른 균들에 비해 마늘즙에 대한 감수성이 상대적으로 낮았다. $65^{\circ}C$로 열처리된 마늘즙은 생마늘즙에 비하여 Bacillus subtilis와 Vibrio parahaemolyticus에 대해 최소저해농도가 0.1%에서 각각 0.2% 및 0.4%의 농도로 다소 높게 나타나 마늘즙을 $65^{\circ}C$에서 30분간 열처리할 경우 일부의 균에 대해 생육저해효과가 다소 저하되었으나, $80^{\circ}C$로 열처리된 마늘즙에서는 생육저해효과가 크게 저하되어 공시된 모든 균주에 대한 최소저해농도가 크게 증가되었다.

Keywords

References

  1. Shin, J.H., Ju. J.C., Kwen, O.C., Yang, S.M. and Lee, S.J. (2004) Physicochemical and physiological activities of garlic from different area. Korean J. Food Nutr., 17, 237-245
  2. Kumar, M. and Berwal, J.S. (1998) Sensitivity of food pathogens to garlic(Allium sativum L.). J. Appl. Microbiol., 84, 213-215 https://doi.org/10.1046/j.1365-2672.1998.00327.x
  3. Song, K. and Milner, J.A. (2001) The influence of heating on the anticancer properties of garlic. J. Nutr., 131, 1054-1057
  4. Briggs, W.H., Xiao, H., Parkin, K.L., Shen, C. and Goldman, I.L. (2000) Differential inhibition of human platelet aggregation by selected allium thiosulfinates. J. Agric. Food Chem., 48, 5731-5735 https://doi.org/10.1021/jf0004412
  5. Gebhardt, R. (1993) Multiple effects of garlic extracts on cholesterol biosynthesis in hepatocytes. Lipids, 28, 613-619 https://doi.org/10.1007/BF02536055
  6. Lau, B.H. (2001) Suppression of LDL oxidation by garlic. J. Nutr., 131, 985-988
  7. Kyo, E., Uda, N., Kasuga, S. and Itakura, Y. (2001) Immunomodunlatory effects of aged garlic extract. J. Nutr., 131, 1075-1079
  8. Kyung, K.H. (2006) Growth inhibitory activity of sulfur compounds of garlic against pathogenic microorganisms. J. Food Hyg. Safety, 21, 145-152
  9. Johnson, M.G. and Vaughn, R.H. (1969) Death of Salmonella typhymurium and Escherichia coli in the presence of freshly reconstituted dehydrated garlic and onion. Appl. Microbiol., 17, 903-905
  10. Saleem, Z.M. and Al-Delaimy, K.S. (1982) Inhibition of Bacillus cereus of garlic extract. J. Food Prot., 45, 1007-1009 https://doi.org/10.4315/0362-028X-45.11.1007
  11. Al-Delaimy, K.S. and Ali, S.H. (1970) Antibacterial action of vegetable extracts on the growth of pathogenic bacteria. J. Sci. Food Agric., 21, 110-112 https://doi.org/10.1002/jsfa.2740210214
  12. Chung, K.S., Kim, J.Y. and Kim, Y.M. (2003) Comparison of antimicrobial activities of garlic juice and heat-treated garlic juice. Korean J. Food Sci. Technol., 35, 540-543
  13. Kim, M.H., Kim, S.Y., Shin, W.S. and Lee, J.S. (2003) Antimicrobial activity of garlic juice against Escherichia coli O157:H7. Korean J. Food Sci. Technol., 35, 752-755
  14. Kim, K.J., Do, J.R. and Kim, H.K. (2005) Antimicrobial, antihypertensive and anticancer activities of garlic extracts. Korean J. Food Sci. Technol., 37, 228-232
  15. Kim, Y.D., Kim, K.M. Hur, C.K., Kim, E.S., Cho, I.K. and Kim, K.J. (2004) Antimicrobial activity of garlic extracts according to different cooking methods. Korean J. Food Preservation, 11, 400-404
  16. Ji, W.D., Jeong, M.S., Choi, U.K., Choi, D.H. and Chung Y.G. (1998) Growth inhibition of garlic(Allium sativum L.) juice on the microorganisms. Agr. Chem. & Biotechnol. 41, 1-5
  17. Chung, K.S., Kang, S.Y. and Kim, J.Y. (2003) Antibacterial activity of garlic extract against pathogenic bacteria and lactic acid bacteria. Korean J. Microbiol. Biotechnol., 31, 32-35
  18. Kim, Y.S., Park K.S., Kyung, K.H., Shim, S.T. and Kim, H.K. (1996) Antibacterial activity of garlic extract against Escherichia coli., Korean J. Food Sci. Technol., 28, 730-735
  19. Stroll, A. and Seebeck, E. (1951) Chemical investigation of alliin, the specific principle of garlic. Adv. Enzymol., 11, 377-400
  20. Small, L.D., Bailey, J.H. and Cavallito, C.J. (1947) Alkyl thiosulfinates. J. Am. Chem. Soc. 69, 1710-1713 https://doi.org/10.1021/ja01199a040
  21. Piddock, L.J.G. (1990) Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. J. Appl. Bacteriol., 68, 307-318 https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  22. Yoon, W.H., Choi, J.H., Lee, K.H. and Kim, C.H. (2005) Antimicrobial and antitumor activities of seed extracts of Camellia sinensis L. Korean J. Food Sci. Technol., 37, 108-112
  23. Koh, M.S. (2004) Antimicrobial activity of Saururus chinensis Bail extract. J. Korean Soc. Food Sci. Nutr., 33, 1098-1105 https://doi.org/10.3746/jkfn.2004.33.7.1098
  24. Lee, B.W. and Shin, D.H. (1991) Screening of natural antimicrobial plant extract on food spoilage microorganisms. Korean J. Food Sci. Technol., 23, 200-204
  25. Kim, J.Y., Lee, Y.C. and Kim, K.S. (2002) Effect of heat treatment on the antimicrobial activities of garlic(Allium sativum). J. Microbiol., Biotechnol., 12, 331-335