Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Screening of a Novel Lactobacilli Replicon from Plasmids of Lactobacillus reuteri KCTC 3678

  • Published : 2008.04.30
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Abstract

A novel lactobacilli replicon from plasmids of Lactobacillus reuteri KCTC 3678 was isolated. Eight L. reuteri strains from Korean Collection for Type Cultures (KCTC) and Korea Food Research Institute (KFRI) were screened for cryptic plasmids and most strains harbored 1 or 2 plasm ids. Particularly, L. reuteri KCTC 3678 contained 6 plasm ids which all were used for screening of lactobacilli replicon. EcoRI digests of the plasmid DNA prep from L. reuteri KCTC 3678 were ligated with pUC19 and the recombinant DNAs were serially named from pLR1 to pLR7. A cat (chloramphenicol acetyltransferase; $Cm^r$) gene originated from pC194 was introduced into pLR1-7, resulting in pLR1cat-pLR7cat, respectively. The recombinant plasmids were introduced into L. reuteri KCTC 3679, and only transformants harboring pLR5cat were obtained, indicating that the insert in pLR5 functioned as a lactobacilli replicon.

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References

  1. Wu CM, Lin CF, Chang YC, Chung TC. Construction and characterization of nisin-controlled expression vectors for use in Lactobacillus reuteri. Biosci. Biotech. Bioch. 70: 757-767 (2006) https://doi.org/10.1271/bbb.70.757
  2. Ma D, Forsythe P, Bienenstock J. Live Lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alphainduced interleukin-8 expression. Infect. Immun. 72: 5308-5314 (2004) https://doi.org/10.1128/IAI.72.9.5308-5314.2004
  3. Saxelin M, Tynkkynen S, Mattila-Sandholm T, de Vos WM. Probiotic and other functional microbes: From markets to mechanisms. Curr. Opin. Biotech. 16: 204-211 (2005) https://doi.org/10.1016/j.copbio.2005.02.003
  4. Doleyres Y, Beck P, Vollenweider S, Lacroix C. Production of 3- hydroxypropionaldehyde using a two-step process with Lactobacillus reuteri. Appl. Microbiol. Biot. 68: 467-474 (2005) https://doi.org/10.1007/s00253-005-1895-4
  5. Lin CF, Ho JL, Chung TC. Characterization of the replication region of the Lactobacillus reuteri plasmid pTC82 potentially used in the construction of cloning vector. Biosci. Biotech. Bioch. 65: 1495-1503 (2001) https://doi.org/10.1271/bbb.65.1495
  6. Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119 (1985) https://doi.org/10.1016/0378-1119(85)90120-9
  7. Moon GS, Pyun YR, Park MS, Ji GE, Kim WJ. Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for bifidobacterial ${\alpha}-amylase$. Appl. Environ. Microb. 71: 5630-5632 (2005) https://doi.org/10.1128/AEM.71.9.5630-5632.2005
  8. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA (1989)
  9. Hallet B, Sherratt DJ. Transposition and site-specific recombination: Adapting DNA cut-and-paste mechanisms to a variety of genetic rearrangements. FEMS Microbiol. Rev. 21: 157-178 (1997) https://doi.org/10.1111/j.1574-6976.1997.tb00349.x
  10. Martín MC, Alonso JC, Suárez JE, Alvarez AE. Generation of foodgrade recombinant lactic acid bacterium strains by site-specific recombination. Appl. Environ. Microb. 66: 2599-2604 (2000) https://doi.org/10.1128/AEM.66.6.2599-2604.2000
  11. De Boever EH, Clewell DB, Fraser CM. Enterococcus faecalis conjugative plasmid pAM373: Complete nucleotide sequence and genetic analyses of sex pheromone response. Mol. Microbiol. 37: 1327-1341 (2000) https://doi.org/10.1046/j.1365-2958.2000.02072.x
  12. Swinfield TJ, Jannière L, Ehrlich SD, Minton NP. Characterization of a region of the Enterococcus faecalis plasmid $pAM{\beta}1$ which enhances the segregational stability of $pAM{\beta}1-derived$ cloning vectors in Bacillus subtilis. Plasmid 26: 209-221 (1991) https://doi.org/10.1016/0147-619X(91)90044-W
  13. Desmond C, Ross RP, Fitzgerald G, Stanton C. Sequence analysis of the plasmid genome of the probiotic strain Lactobacillus paracasei NFBC338 which includes the plasmids pCD01 and pCD02. Plasmid 54: 160-175 (2005) https://doi.org/10.1016/j.plasmid.2005.04.001
  14. Kanatani K, Tahara T, Oshimura M, Sano K, Umezawa C. Identification of the replication region of Lactobacillus acidophilus plasmid pLA103. FEMS Microbiol. Lett. 133: 127-130 (1995) https://doi.org/10.1111/j.1574-6968.1995.tb07872.x
  15. del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz- Orejas R. Replication and control of circular bacterial plasmids. Microbiol. Mol. Biol. R. 62: 434-464 (1998)
  16. Jeong D-W, Hwang E-S, Lee HJ. Food-grade expression and secretion systems in Lactococcus. Food Sci. Biotechnol. 15: 485-493 (2006)
  17. Kim J-T, Jung H-Y, Lee N-K, Rhim S-L, Paik H-D. Isolation, identification, and probiotic properties of Lactobacillus reuteri HY701 from human feces. Food Sci. Biotechnol. 15: 677-682 (2006)