Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-inflammatory Effects of Enzymatic Extract from Ecklonia cava on TPA-induced Ear Skin Edema

  • Ahn, Ginnae (Department of Marine Life Science, Cheju National University) ;
  • Park, Eun-Jin (College of Veterinary Medicine and Institute of Medical Science, Cheju National University) ;
  • Kim, Dae-Seung (Department of Marine Life Science, Cheju National University) ;
  • Jeon, You-Jin (Faculty of Marine Medicine Science, Cheju National University) ;
  • Shin, Tae-Kyun (College of Veterinary Medicine and Institute of Medical Science, Cheju National University) ;
  • Park, Jae-Woo (Department of Nuclear and Energy Engineering, Cheju National University) ;
  • Woo, Ho-Chun (College of Veterinary Medicine and Institute of Medical Science, Cheju National University) ;
  • Lee, Ki-Wan (Department of Marine Life Science, Cheju National University) ;
  • Jee, Young-Heun (College of Veterinary Medicine and Institute of Medical Science, Cheju National University)
  • Published : 2008.08.31
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Abstract

Anti-inflammatory potential of the enzymatic extract prepared by Kojizyme (ECK), a component of brown seaweeds Ecklonia cava (Alariaceae, Phaeophyta) in vivo was investigated. For the application of mouse ear edema model, 12-O-tetradecanoylphorbol acetate (TPA) was used, a topical inducer of a long-lasting inflammatory response. Our results demonstrated that ECK inhibited ear edema when topically applied to mouse ear skin. In histological evaluation, the inhibition activity of ECK on TPA-induced inflammation is similar to that of dexamethasone, although less strong. In addition, the mRNA expression levels of IL-$1{\beta}$, IFN-$\gamma$, TNF-$\alpha$, and cyclooxygenase-2 (COX2) and the immunoreactivity to inducible nitric oxide synthase (iNOS) and COX2 expressed mainly in inflammatory cells were down-regulated by ECK. These results indicate that ECK has anti-inflammatory effects through the inhibition of Th1 cytokines and 2 inducers of inflammation in TPA-induced ear skin edema.

Keywords

References

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