S-Adenosyl-L-methionine (SAM) Production by Lactic Acid Bacteria Strains Isolated from Different Fermented Kimchi Products

  • 발행 : 2008.08.31

초록

S-Adenosyl-L-methionine (SAM) is a bioactive material used in the treatment of depression, osteoarthritis, and liver disease. To obtain lactic acid bacteria (LAB) producing high concentrations of SAM, LAB were isolated from commercial kimchi and from prepared kimchi products that contained shrimp jeotgal (fermented salty seafood) or sand lance jeotgal or that were fermented at 5 or $10^{\circ}C$, respectively, when pH was 4.2 to 4.8 and titratable acidity 0.6 to 0.9. Among the 179 LAB strains isolated from the fermented kimchi products, the genus Leuconostoc produced the highest intracellular level of SAM (1.58 mM) and Lactobacillus produced the second highest level (up to 1.47 mM) in the strain culture. This is the first study to quantify SAM in LAB isolated from fermented kimchi prepared by a general kimchi recipe. Ultimately, the selected strains (Leuconostoc mesentroides subsp. mesenteroides/dextranicum KSK417, L. mesentroides subsp. mesenteroides/dextranicum KJM401, and Lactobacillus bifermentans QMW327) could be useful as starters to manufacture fermented foods containing high levels of SAM.

키워드

참고문헌

  1. Lee CW, Kop CY, Ha DM. Microfloral changes of the lactic acid bacteria during kimchi fermentation and identification of the isolates. Korean J. Appl. Microbiol. Biotechnol. 20: 102-109 (1992)
  2. Kim M, Chun J. Bacterial community structure in kimchi, a Korean fermented vegetable food, as reveasled by 16S rRNA gene analysis. Int. J. Food Microbiol. 103: 91-96 (2005) https://doi.org/10.1016/j.ijfoodmicro.2004.11.030
  3. Lee CH. Lactic acid fermented foods and their benefits in Asia. Food Control 8: 259-269 (1997) https://doi.org/10.1016/S0956-7135(97)00015-7
  4. Um S, Shin WS, Lee JH. Real-time PCR monitoring of Lactobacillus sake, Lactobacillus plantarum, and Lactobacillus paraplantarum during kimchi fermentation. Food Sci. Biotechnol. 15: 595-598 (2006)
  5. Choi SY, Lee MK, Choi KS, Koo YJ, Park WS. Changes of fermentation characteristics and sensory evaluation of kimchi on different storage temperature. Korean J. Food Sci. Technol. 30: 644-649 (1998)
  6. Ko YT, Baik IH. Changes in pH, sensory properties, and volatile odor components of kimchi by heating. Korean J. Food Sci. Technol. 34: 1123-1126 (2002)
  7. Lee JH, Kweon DH, Lee SC. Isolation and characterization of an immunopotenitating factor from Lactobacillus plantarum in kimchi: Assessment of immunostimulatory activities. Food Sci. Biotechnol. 15: 877-883 (2006)
  8. Lee KH, Hwang JH, Yu KW. Preparation of kimchi supplemented with immunomodulatory components isolated from licorice. Food Sci. Biotechnol. 12: 351-357 (2003)
  9. Woo SM, Jeong YJ. Effect of germinated brown rice concentrate on free amino acid levels and antioxidant and nitrate scavenging activity in kimchi. Food Sci. Biotechnol. 15: 351-356 (2006)
  10. Jayaprakasha HM, Yoon YC, Paik HD. Probiotic functional dairy foods and health claims: An overview. Food Sci. Biotechnol. 14: 523-528 (2005)
  11. Lu SC. S-Adenosyl-methionine. Int. J. Biochem. Cell. B. 32: 391-395 (2000) https://doi.org/10.1016/S1357-2725(99)00139-9
  12. Poirier LA. Methyl group deficiency in hepatocarcinogenesis. Drug Metab. Rev. 26: 185-199 (1994) https://doi.org/10.3109/03602539409029790
  13. Kim DJ, Huh JH, Yang YY, Kang CM, Lee IH, Hyun CG, Hong SK, Suh JW. Accumulation of S-adenosyl-L-methionine enhances production of actinorhodin but inhibits sporulation in Streptomyces lividans TK23. J. Bacteriol. 185: 592-600 (2003) https://doi.org/10.1128/JB.185.2.592-600.2003
  14. Shiozaki S, Shimizu S, Yamada H. Unusual interacellular accumulation of S-adenosyl-L-methionine by microorganisms. Agr.Biol. Chem. 48: 2293-2300 (1984) https://doi.org/10.1271/bbb1961.48.2293
  15. Hong SI, Park WS. Use of color indicators as an active packaging system for evaluating kimchi fermentation. J. Food Eng. 46: 67-72 (2000) https://doi.org/10.1016/S0308-8146(00)00141-2
  16. Miyao S, Ogawa T. Selected media for enumerating lactic acid bacteria groups from fermented pockles. Nippon Shokuhin Kogyo Gakk. 35: 610-617 (1988) https://doi.org/10.3136/nskkk1962.35.9_610
  17. Okamoto S, Lezhava A, Hosaka T, Okamoto-Hosoya Y, Ochi K. Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3. J. Bacteriol. 185: 601-609 (2003) https://doi.org/10.1128/JB.185.2.601-609.2003
  18. Payne SH, Ames BN. A procedure for rapid extraction and highpressure liquid chromatographic separation of the nucleotides and other small molecules from bacterial cells. Anal. Biochem. 123: 151-161 (1982) https://doi.org/10.1016/0003-2697(82)90636-4
  19. Rhee JN, Lyoo YW, Choi MU. Laboratory scale preparation of Sadenosyl-L-methionine from yeast. Korean J. Appl. Biotechnol. 19: 588-591 (1991)
  20. Graham DE, Bocks CL, Schalk-Hihi C, Lu ZJ, Markham GD. Identification of a highly diverged class of S-adenolsylmethionine synthetases in the Archaea. J. Biol. Chem. 275: 4055-4059 (2000) https://doi.org/10.1074/jbc.275.6.4055
  21. Creason GL, Madison JT, Thompson JF. Soybeans and radish leaves contain only one of the sulfonium diastereoisomers of Sadenolsylmethionine. Phytochemistry 24: 1151-1155 (1985) https://doi.org/10.1016/S0031-9422(00)81092-4