Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지

THE ANTIMICROBIAL EFFECT OF HORSERADISH(ARMORACIA RUSTICANA) ROOT EXTRACTS AGAINST STREPTOCOCCUS MUTANS ISOLATED FROM HUMAN DENTAL PLAQUE

치태에서 분리된 Streptococcus mutans에 대한 서양산 고추냉이(Armoracia rusticana) 뿌리 추출물의 항균효과

  • Kim, Hye-Kyoung (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University) ;
  • Park, Ho-Won (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University) ;
  • Shin, Il-Shik (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Lee, Ju-Hyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University) ;
  • Seo, Hyun-Woo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University)
  • 김혜경 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 신일식 (강릉대학교 해양생명공학부 식품미생물학연구실) ;
  • 이주현 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소)
  • Published : 2008.05.30
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Abstract

Recently interesting in development of antimicrobial agent from natural origin has been increased in these days. Many studies have been reported antimicrobial effect of Horseradish(Armoracia rusticana) root extracts against various microorganisms such as Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio parahaemolyticu, Staphylococcus aureus, Bacillus subtilis, Aspergillus parasiticus, Helicobacter pylori. The main component related to antimicrobial activity in horseradish is well known as allyl isothiocyanate(AIT). In this study, we investigated the antimicrobial effects of Horseradish(Armoracia rusticana) root extracts against Streptococcus mutans isolated from human dental plaque, Streptococcus mutans reference strain and compared with that of chlorhexidine. Horseradish root extracts and chlorhexidine were tested to determine their minimum inhibitory concentration( MIC) and minimum bactericidal concentration(MBC). The result of this study can be summerized as follows; 1. Horseradish root extracts showed antimicrobial effect against both S. mutans isolated strain and reference strain, their MIC were respectively $0.083{\sim}0.25%$ ($833.33{\sim}2500 ppm$), 0.25% (2500 ppm). Horseradish root extracts showed antimicrobial effect against S. mutans isolated strain at same or slightly lower concentration compared with MIC of reference strain. 2. $0.083{\sim}0.25%$ horseradish root extracts showed similar antimicrobial effect with chlorhexidine ($0.0021{\sim}0.0041%$).

천연항균제의 개발에 대한 관심이 날로 증가하는 가운데, 다수의 연구들에서 Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus subtilis, Aspergillus parasiticus, Helicobacter pylori 등 다양한 세균들에 대한 서양산 고추냉이의 항균 효과가 밝혀진 바 있다. 서양산 고추냉이가 치아우식증의 원인균인 Streptococcus mutans에 대해서도 항균성을 나타낸다는 보고가 있으나, 아직까지 구강내에서 분리된 임상분리균주를 대상으로 한 연구는 미진한 실정이다. 본 연구에서는 사람의 구강내에서 분리 및 동정된 Streptococcus mutans에 대한 서양산 고추냉이 뿌리 추출물의 항균 효과를, 대표적 항균제인 클로르헥시딘과 비교하여 알아보고, Streptococcus mutans 표준균주에 대한 서양산 고추냉이 뿌리 추출물의 효과와 비교하고자 하였다. 항균 효과를 평가하기 위해 최소억제농도와 최소살균농도를 측정하여 다음과 같은 결론을 얻었다. 1. 본 연구에서 Streptococcus mutans 임상 분리균주와 표준균주에 대한 서양산 고추냉이 뿌리 추출물의 최소억제 농도는 각각 평균 $0.083{\sim}0.25%$ ($833.33{\sim}2500 ppm$), 평균 0.25%(2500 ppm)으로 나타나, 두 종류의 균주에 대한 항균 효과에는 뚜렷한 차이가 없는 것으로 나타났다. 2. 서양산 고추냉이 뿌리 추출물은 $0.083{\sim}0.25%$의 농도에서 클로르헥시딘($0.0021{\sim}0.0041%$)과 대등한 항균 효과를 가지는 것으로 나타났다.

Keywords

References

  1. Fitzgerald RJ, Keyes PH : Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc, 61:9-19, 1960. https://doi.org/10.14219/jada.archive.1960.0138
  2. Michalek SM, McGhee JR, Shiota T, et al. : Virulence of Streptococcus mutans : Cariogenicity of S. mutans in adult gnotobiotic rats. Infect Immun, 15:466-471, 1977.
  3. De Stoppelaar JD, Van Houte J, Backer Dirks O : The relationship between extracellular polysaccharide- producing streptococci and smooth surface caries in 13-year-old children. Caries Res, 3:190- 199, 1969. https://doi.org/10.1159/000259582
  4. Krasse B : Relationship between caries activity and the number of lactobacilli in the oral cavity. Acta Odontol Scand, 12:157-172, 1954. https://doi.org/10.3109/00016355409026083
  5. Jordan HV, Keyes PH, Bellack S : Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin. J Periodontal Res, 7:21-28, 1972. https://doi.org/10.1111/j.1600-0765.1972.tb00627.x
  6. Gibbons RJ, Nygaard M : Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque forming streptococci. Arch Oral Biol, 3:1249-1262, 1968.
  7. Loesche WJ : Dental Caries. A treatable infection. Charles C Thomas, Publisher, Spring field, 1982.
  8. Whiley RA, Beighton D : Current classification of the oral streptococci. Oral Microbiol Immunol, 13:195-216, 1998. https://doi.org/10.1111/j.1399-302X.1998.tb00698.x
  9. 임성훈, 서정순, 윤영주 등 : 녹차 및 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균 작용. 대한치과교정학회지, 33:381-389, 2003.
  10. Loesche WJ : Role of Streptococcus mutans in human dental decay. Microbiol Rev, 50:353-380, 1986.
  11. van Houte J : Role of micro-organisms in caries etiology. J Dent Res, 73:672-681, 1994. https://doi.org/10.1177/00220345940730031301
  12. Kohler B, Andreen I, Jonsson B : The earlier the colonization by mutans streptococci, the higher the caries prevalence at 4 years of age. Oral Microbiol Immunol, 3:14-17, 1988. https://doi.org/10.1111/j.1399-302X.1988.tb00598.x
  13. Coykendall AL : Classification and identification of the viridans streptococci. Clin Microbiol Rev, 2:315- 328, 1989. https://doi.org/10.1128/CMR.2.3.315
  14. Carlsson P, Gaudour IA, Olsson B, et al. : High prevalence of mutans streptococci in a population with extremely low prevalence of dental caries. Oral Microbiol Immunol, 2:121-124, 1987. https://doi.org/10.1111/j.1399-302X.1987.tb00274.x
  15. Fujiwara T, Sasada E, Mima N, et al. : Caries prevalence and salivary mutans streptococci in 0-2- year-old children of Japan. Community Dent Oral Epidemiol, 19:151-154, 1991. https://doi.org/10.1111/j.1600-0528.1991.tb00131.x
  16. Hirose H, Hirose K, Isogai E, et al. : Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment. Caries Res, 27:292-297, 1993. https://doi.org/10.1159/000261553
  17. 안승태, 박재홍, 이긍호: 6세 이하의 어린이에서 Streptococcus mutans와 Streptococcus sobrinus의 분포에 관한 연구. 대한소아치과학회지, 32:207-215, 2005.
  18. Becker MR, Paster BJ, Leys EJ, et al. : Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol, 40:1001-1009, 2002. https://doi.org/10.1128/JCM.40.3.1001-1009.2002
  19. Weitz M, Brownstein G, Deasy M : Effect of a twice daily 0.12% chlorhexidine rinse on the oral health of a geriatric population. Clin Prev Dent, 14:9-13, 1992.
  20. Loe H, Schiott CR : The effect of mouthrinses and topical applications of chlorhexidine on the development of dental plaque and gingivitis in man. J Periodontal Res, 5:79-83 1970. https://doi.org/10.1111/j.1600-0765.1970.tb00696.x
  21. Loe H, Schiott CR, Karring G, et al. : Two years oral use of chlorhexidine in man. 1. General design and clinical effects. J Periodontal Res, 11:135-144, 1976. https://doi.org/10.1111/j.1600-0765.1976.tb00061.x
  22. Lang NP, Hotz P, Graf H. et al. : Effects of super-vised chlorhexidine mouthrinses in children. A longitudinal clinical trial. J Periodontal Res, 17:101-111, 1982. https://doi.org/10.1111/j.1600-0765.1982.tb01135.x
  23. Grossman E, Reiter G, Sturzenburger OP, et al. : Six-month study of the effects of a chlorhexindine mouthrinse on gingivitis in adults. J Perio Res, 21:33-43, 1986 https://doi.org/10.1111/j.1600-0765.1986.tb01513.x
  24. Overholser CD, Meiller TF, DePaola LG, et al. : Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol, 17:575-579, 1990. https://doi.org/10.1111/j.1600-051X.1990.tb01108.x
  25. Featherstone JD, Zero DT : Laboratory and human studies to elucidate the mechanism of action of fluoride- containing dentifrices. In: Embery G, Rolla R, eds. Clinical and biological aspects of dentifrices. Oxford, England: Oxford University Press; 1992;41-50.
  26. Lagerlof F, Oliveby A : Clinical implications : new strategies for caries treatment. In: Stookey GH, Beiswanger B, eds. Indiana Conference 1996: Early Detection of Dental Caries. Indianapolis: Indiana University School of Dentistry; 1996.
  27. Hennessey TS : Some antibaterial properties of chlorhexidine. J Periodonto Res, 8:61-67, 1973. https://doi.org/10.1111/j.1600-0765.1973.tb02166.x
  28. Budtz-Jorgensen E, Loe H : Chlorhexidine as a denture disinfectant in the treatment of denture stomatitis. Scand J Dent Res, 80:457-464, 1972.
  29. Davies A : The mode of action of chlorhexidine. J Periodont Res, 8:68-75, 1973. https://doi.org/10.1111/j.1600-0765.1973.tb02167.x
  30. Schaupp H, Wohnaut H : Disturbance of taste from oral disinfectants. HNO, 26:335-341, 1978.
  31. Bergstorm J, Holmberg B : The effect of chlorhexidine emulsion on plaque. An infra individual study of local applicatin. Swed Dent J, 66:461-465, 1973.
  32. Gildemeister E, Fr Hoffmann : The volatile oils. John Wiely & Sons Inc. 2nd ed., p516, 1961.
  33. Hitomi Kumagai : Analysis of volatile components in essential oil of upland wasabi and their inhibitory effects on platelet aggregation. BiosciBiotechBiochem, 58:2131-2135, 1994.
  34. Kazuo Ina : Volatile components of wasabi (Wasabia japonica) and horseradish (Cocholeria armoracia). Nippon Shokuhin Kagyo Gakkaishi, 18:365-370, 1981.
  35. Nishida M : Studies on the pungent component. Antibacterial properties of essential oil of Eutrema wasabi Maxim. Yakugaku Zasshi, 78:435-443, 1958. https://doi.org/10.1248/yakushi1947.78.4_435
  36. Inoue S, Goi H, Miyauchi K, et al. : Inhibitory effect of volatile constituents of plants on the proliferation of bacteria-Antibacterial activity of plant volatiles. J Antibact Antifung Agents, 11:609-615, 1983.
  37. Hasegawa N, Matsumoto Y, Hoshino A, et al. : Comparison of effects of Wasabia japonica and allyl isothiocyanate on the growth of four strains of Vibrio parahaemolyticus in lean and fatty tuna meat suspensions. Int J Food Microbiol, 49:27-34, 1999. https://doi.org/10.1016/S0168-1605(99)00043-4
  38. Il Shik Shin, Hideki Masuda, Kinae Naohide : Bactericidal activity of wasabi(Wasabia japonica) against Helicobacter pylori. Int J Food Microbiol, 94:255-261, 2004. https://doi.org/10.1016/S0168-1605(03)00297-6
  39. 신일식, 이정모 : 고추냉이 뿌리의 항균활성 및 항변이원활성에 대한 연구. 한국수산학회지, 31:835-841, 1998.
  40. Masuda H, Inoue T, Kobayashi Y : Anticaries effect of Wasabi components. ACS symposium series 859:142-153, 2003. https://doi.org/10.1021/bk-2003-0859.ch009
  41. 유난영, 박호원, 이주현 등 : 구강내 미생물에 대한 서양산 고추냉이 (Armoracia rusticana) 뿌리 추출물의 항균 효과. 대한소아치과학회지, 33:447-456, 2006.
  42. Bamba H, Kondo Y, Wong RM, et al. : Evaluation of an assay method of the susceptibility of antimicrobial agents using a 96-well flat-bottom microplate and a microplate reader. Am J Gastroenterol, 92:659-664, 1997.
  43. Fenwick GR, Heaney RK, Mullin WJ : Glucosinolates and their breakdown products in food and food plants. Crit Rev Food Sci Nutr, 18:123- 201, 1983. https://doi.org/10.1080/10408398209527361
  44. Verhoeven DT, Verhagen H, Goldbohm RA, et al. : A review of mechanisms underlying anticarcinogenicity by brassica vegetables, Chem Biol Interact, 103:79-129, 1997. https://doi.org/10.1016/S0009-2797(96)03745-3
  45. Kawakishi S, T Kaneko : Interaction of oxidized glutathione with allyl isothiocyanate. Phytochemistry, 24:715-718, 1985. https://doi.org/10.1016/S0031-9422(00)84882-7
  46. Kawakishi S, T Kaneko : Interaction of proteins with allyl isothiocyanate. J Agri Food Chem, 35:85- 88, 1987. https://doi.org/10.1021/jf00073a020
  47. Kojima M, K Ogawa : Studies on the effect of isothiocyanates and their analogues on microorganisms: (I) effects of isothiocyanates on the oxygen uptake of yeasts. J Ferment Technol, 49:740-746, 1971.
  48. Ochman H, Lerat E, Daubin V : Examining bacterial species under the specter of gene transfer and exchange. Proc Natl Acad Sci USA, 102:6595-6599, 2005. https://doi.org/10.1073/pnas.0502035102
  49. Clarridge JE 3rd : Impact of 16s rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev, 17:840-862, 2004. https://doi.org/10.1128/CMR.17.4.840-862.2004
  50. Bauroth K, Charles CH, Mankodi SM, et al. : The efficacy of an essential oil antiseptic mouthrinse vs. dental floss in controlling interproximal gingivitis : a comparative study. J Am Dent Assoc, 134:359-365, 2003. https://doi.org/10.14219/jada.archive.2003.0167
  51. Fornell J, Sundin Y, Lindhe J. : Effect of $Listerine^{\circledR}$ on dental plaque and gingivitis. Scand J Dent Res, 83:18-25, 1975
  52. Lusk SS, Bower GM, Tow HD, et al. : Effects of an oral rinse on experimental gingivitis, plaque formation, and formed plaque. J Am Soc Prev Dent, 4:31- 34, 1974.
  53. Sharma N, Charles CH, Lynch MC et al. : Adjunctive benefit of an essential oil-containing mouthrinse in reducing plaque and gingivitis in patients who brush and floss regularly. A six-month study. J A Dent Asso, 135:496-504, 2004. https://doi.org/10.14219/jada.archive.2004.0217