Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative and Hepatoprotective Effects of Acer tegmentosum M. Extracts

산겨릅나무 추출물의 항산화 및 간 기능 보호효과

  • 권하나 (영남대학교 식품영양학과) ;
  • 박정륭 (영남대학교 식품영양학과) ;
  • 전정례 (영남대학교 식품영양학과)
  • Published : 2008.11.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate antioxidative and hepatoprotective effects of Acer tegmentosum M. (ATM) extracts. Content of total polyphenol of EtOAc fraction was the highest amount among fractions. Electron donating abilities of all fractions were increased as concentrations of each fraction were increased. ATM BuOH fraction showed the highest SOD like activity at low concentration (<$250\;{\mu}g/mL$). To investigate the protective effect of ATM on hepatotoxicity, ATM BuOH fraction was administered to mice for 7 consecutive days, and then lipopolysaccharide (LPS) was injected at a dosage of 1 mg/kg. The LPS led to increase of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and blood urea nitrogen (BUN). However, pretreatment with BuOH fraction prior to LPS injection significantly decreased plasma hepatospecific enzyme levels. Histological findings demonstrated that pretreatment with BuOH fraction resulted in an attenuation of LPS induced liver damages.

본 연구는 산겨릅나무의 식품학적 가치를 알아보고 기능성식품 소재로서의 이용.개발을 위하여 항산화 및 간 기능 보호효과를 탐색하였다. 항산화성 결과 가운데 총 폴리페놀은 EtOAc fr.에서 함량이 가장 많았고 BuOH fr., EtOH ex., $CHCl_3$ fr., hot water ex., aqueous fr. 순으로 높게 나타났다. 전자공여능은 EtOAc fr.과 BuOH fr.이 ascorbic acid와 유사한 전자공여능을 보였으며 SOD 유사활성능은 $250\;{\mu}g/mL$ 이하의 농도에서는 BuOH fr.이 가장 높게, 그 이상에서는 aqueous fr.에서 가장 높게 나타났다. LPS로 급성 간 손상을 유도한 마우스에서의 BuOH fr.의 간 기능 보호효과에서는 BuOH fr.을 전 처치한 다음 LPS를 투여한 군이 LPS만을 투여한 군보다 혈장 AST와 ALT의 활성은 유의하게 감소 되었으나 BUN의 농도는 유의성이 관찰되지 않았다. 간조직의 형태학적 관찰에서 BuOH fr.을 투여하고 LPS로 급성 간 손상을 유도한 군의 간세포는 염증과 괴사가 나타났지만 LPS군에 비하여 그 정도가 적은 것으로 나타났다.

Keywords

References

  1. Ito N, Hirose M, Fukeshima S, Tsuda H, Shirai T, Tatematsu M. 1986. Their carcinogenic and modifying effects on chemical carcinogenesis. Food Chem Toxicol 24: 1071-1082 https://doi.org/10.1016/0278-6915(86)90291-7
  2. Lee KW, Nam BH, Jo WS, Oh SJ, Kang EY, Choi YJ, Lee JY, Cheon SC, Jeong MH, Lee JD. 2006. Collection, classification, and hepatic effect of native Cordyceps militaris. Korea J Mycol 34: 7-13 https://doi.org/10.4489/KJM.2006.34.1.007
  3. Di Cario G, Mascolo N, Izzo AA, Capasso F. 1999. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci 65: 337-353 https://doi.org/10.1016/S0024-3205(99)00120-4
  4. Yang YM, Hyun JW, Lim KH, Sung MS, Kang SS, Park WH, Bae K, Cho WH, Kim HJ, Woo ER, Park HK, Park JG. 1996. Antineoplastic effect of extracts from traditional medicinal plants and various plants (III). Kor J Pharmacogn 27: 105-110
  5. Sunami E, Tsuno N, Osada T, Saito S, Kitayama J, Tomozawa S, Tsuruo T, Shibata Y, Muto T, Nagawa H. 2000. MMP-1 is a prognostic marker for hematogenous metastasis of colorectal cancer. The Oncologist 5: 108-114 https://doi.org/10.1634/theoncologist.5-2-108
  6. Kim TW. 1996. The woody plants of Korea in color. Kyohak Co., Seoul. p 476
  7. Kwon DJ, Bae YS. 2007. Phenolic compounds from Acer tegmentosum bark. Wood Sci Technol 35: 145-151
  8. Hur JM, Yang EJ, Choi SH, Song KS. 2006. Isolation of phenolic glucosides from the stems of Acer tegmentosum Max. J Korean Soc Appl Biol Chem 49: 149-152
  9. Hur JM, Jun MR, Yang EJ, Choi SH, Park JC, Song KS. 2007. Isolation of isoprenoidal compounds from the stems of Acer tegmentosum Max. Kor J Pharmacogn 38: 67-70
  10. Hong BK, Eom SH, Lee CO, Lee JW, Jeong JH, Kim JK, Cho DH, Yu CY, Kwon YS, Kim MJ. 2007. Biological activities and bioactive compounds in the extract of Acer tegmentosum Maxim. stem. Korean J Medicinal Crop Sci 15: 296-303
  11. Shin IC, Sa JH, Shim TH, Lee JH. 2006. The physical and chemical properties and cytotoxic effects of Acer tegmentosum Maxim. extracts. J Korean Soc Appl Biol Chem 49: 322-327
  12. Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16: 144-158
  13. Blois ML. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1224 https://doi.org/10.1038/1811199a0
  14. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  15. Kuramoto T. 1992. Development and application of food materials from extract such as SOD. Food Process 27: 22-23
  16. Bret-Dibat JL, Creminon C, Couraud JY, Kellev KW, Dantzer R, Kent S. 1997. Systemic capsaicin pretreatment fails to block the decrease in food-motivated behavior induced by lipopolysaccharide and interleukin-1b. Brain Res Bull 42: 443-449 https://doi.org/10.1016/S0361-9230(96)00370-X
  17. Wang JH, Redmond HP, Watson RW, Bouchier-Hayes D. 1995. Role of lipopolysaccharide and tumor necrosis factor-a in induction of hepatocyte necrosis. Am J Physiol 269: 297-304
  18. Sewerynek E, Melchiorri D, Reiter RJ, Ortiz GG, Lewinski A. 1995. Lipopolysaccharide-induced hepatotoxicity is inhibited by the antioxidant melatonin. Eur J Pharmacol 293: 327-334 https://doi.org/10.1016/0926-6917(95)90052-7
  19. Khasina EI, Sqrebneva MN, Ermak IM, Maleev VV. 2007. Effect of carrageenan on non-specific resistance to LPSinduced endotoxemia in mice. Zh Mikrobiol Epidemiol Immunobiol 2: 57-60
  20. Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28: 56-63 https://doi.org/10.1093/ajcp/28.1.56
  21. Uchida K, Tejima K. 1974. Use of a mixture of a phenol reagent and a urease solution in the urease-indophenol method (analysis of blood urea nitrogen). Rinsho Byori 22: 207
  22. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. 2000. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower. J Korean Soc Food Sci Nutr 29: 1127-1132
  23. Sa JH, Jin YS, Shin IC, Shim TH, Wang MH. 2004. Photoprotective effect and antioxidative activity from different organs of Morus bombycis K. Kor J Pharmacogn 35: 207-214
  24. Lee SY, Hwang EJ, Kim GH, Choi YB, Lim CY, Kim SM. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of Camellia japonica L. Korean J Medicinal Crop Sci 13: 93-100
  25. Lee YS. 2007. Physiological activities of hot water extract from Ailanthus altissima. Korean J Food Preserv 14: 170-176
  26. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338
  27. Jeong SJ, Lee JH, Song HN, Seong NS, Lee SE, Baeg NI. 2004. Screening for antioxidant activity of plant medicinal extracts. J Korean Soc Appl Biol Chem 47: 135-140
  28. Kwon GJ, Choi DS, Wang MH. 2007. Biological activities of hot water extracts from Euonymus alatus leaf. Korean J Food Sci Technol 39: 569-574
  29. Stellato T, Rhodes RS, McDougal WS. 1980. Azotemia in upper gastrointestinal hemorrhage. Am J Gastroenterol 73: 486-489
  30. Jeon JR, Park JR. 2002. Effect of Eucommia ulmoides leaf water extract on hepatotoxicity of carbon tetrachloride-induced rats. J Korean Soc Food Sci Nutr 31: 124-130 https://doi.org/10.3746/jkfn.2002.31.1.124

Cited by

  1. Fermentation Process for Odor Removal of Oyster (Crassostrea gigas) Hydrolysate and Its Properties vol.45, pp.4, 2016, https://doi.org/10.3746/jkfn.2016.45.4.542
  2. Hepatoprotection of different water extracts from Acer tegmentosum M. on CCl4-induced acute hepatotoxicity in mice: comparative efficacies between the extracts of boughs, twigs, and leaves vol.7, pp.4, 2011, https://doi.org/10.1007/s13273-011-0051-8
  3. Antioxidant and Alcohol Degradation Activities of Extracts from Acer tegmentosum Maxim. vol.42, pp.3, 2013, https://doi.org/10.3746/jkfn.2013.42.3.378
  4. Anticytotoxic and Radical Scavenging Activities of Acer tegmentosum Maxim Stem Extracts vol.24, pp.11, 2015, https://doi.org/10.5322/JESI.2015.24.11.1315
  5. Antioxidative Activities of the Codonopsis lanceolata Extract in vitro and in vivo vol.39, pp.2, 2010, https://doi.org/10.3746/jkfn.2010.39.2.193
  6. Evaluation of antioxidant, α-glucosidase inhibition and acetylcholinesteraseinhibition activities of Allium hookeri root grown in Korea and Myanmar vol.23, pp.2, 2016, https://doi.org/10.11002/kjfp.2016.23.2.239
  7. Traditional uses, phytochemistry, and pharmacology of the genus Acer (maple): A review vol.189, 2016, https://doi.org/10.1016/j.jep.2016.04.021
  8. Biological Activities of Ethanol Extracts from Monascus-Fermented Chinese Yam vol.21, pp.8, 2011, https://doi.org/10.5352/JLS.2011.21.8.1142
  9. Anti-Diabetic, Alcohol Metabolizing Enzyme, and Hepatoprotective Activity of Acer tegmentosum Maxim. Stem Extracts vol.44, pp.12, 2015, https://doi.org/10.3746/jkfn.2015.44.12.1785
  10. Anti-Oxidative and Anti-Inflammatory Effects of the Solvent Fraction from Theobroma cacao L. Extract vol.22, pp.10, 2012, https://doi.org/10.5352/JLS.2012.22.10.1330
  11. Verification of anti-oxidative activity of Aruncus dioicus, a native plant of Ulleungdo vol.42, pp.1, 2015, https://doi.org/10.5010/JPB.2015.42.1.55
  12. A Comparison of Antioxidant Effects among Non-fermented and Fermented Columbian Coffee, and Luwak Coffee Beans vol.30, pp.6, 2014, https://doi.org/10.9724/kfcs.2014.30.6.757
  13. Biological Activities of Fermented Dioscorea batatas Dence by two Stage Fermentation vol.48, pp.1, 2012, https://doi.org/10.7845/kjm.2012.48.1.029
  14. Characteristics of Culture of Oyster Mushroom (Pleurotus ostreatus) on Addition Rate of Acer tegmentosum vol.41, pp.1, 2013, https://doi.org/10.4489/KJM.2013.41.1.21
  15. 벌나무 잎의 영양성분 및 이화학 특성 vol.22, pp.8, 2016, https://doi.org/10.20878/cshr.2016.22.8.003
  16. 해방풍 부위별 용매추출물의 항산화 활성 및 nitric oxide 생성 억제 vol.24, pp.1, 2017, https://doi.org/10.11002/kjfp.2017.24.1.116
  17. 마가목 줄기 열수 및 에탄올 추출물의 항산화 활성 vol.32, pp.3, 2008, https://doi.org/10.6116/kjh.2017.32.3.29
  18. Antioxidant activity of ethanol extracts from the root and bark of Ulmus davidiana vol.51, pp.2, 2008, https://doi.org/10.9721/kjfst.2019.51.2.133
  19. Anti-inflammatory Effect of Hot Water Extracts of Acer tegmentosum Maxim Extracted Under Optimal Conditions for Antioxidant Activity on LPS-Induced RAW 264.7 Cells vol.32, pp.2, 2021, https://doi.org/10.7856/kjcls.2021.32.2.269