Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibition Effect of 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)Propionic Acid in Kimchi with Anti-Atherogenic Activity on the Accumulation of Lipids in the Organs of ApoE(-/-) Mice

ApoE(-/-) Mice에서 배추김치 활성 성분인 3-(4'-hydroxyl-3',5'-dimethoxylphenyl)Propionic Acid의 장기 지질저하 효과

  • Kim, Hye-Yeon (Dept. of Food Science & Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Noh, Jeong-Sook (Dept. of Food Science & Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Song, Yeong-Ok (Dept. of Food Science & Nutrition, and Kimchi Research Institute, Pusan National University)
  • 김혜연 (부산대학교 생활환경대학 식품영양학과 및 김치연구소) ;
  • 노정숙 (부산대학교 생활환경대학 식품영양학과 및 김치연구소) ;
  • 송영옥 (부산대학교 생활환경대학 식품영양학과 및 김치연구소)
  • Published : 2008.11.28
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Abstract

Preventive effects of 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)propionic acid (HDMPPA), an active compound in Korean cabbage kimchi with anti-atherogenic effects, on the accumulation of lipids in the vital organs of $apoE^{(-/-)}$ mice fed atherogenic diet (AD) were studied. Each group of 10 mice was fed AD for 8 weeks with intraperitoneal injection of either HDMPPA (1 mg HDMPPA/100 g BW/day) or phosphate buffered saline as a vehicle. The organs used for this study were liver, kidney, spleen, lung, testis, and brain. Total cholesterol (TC) concentration of lung was the highest followed by spleen and brain. TC level for the liver was the lowest. In contrast to the results of TC, triglyceride (TG) concentration in the liver was the highest followed by kidney and testis. $ApoE^{(-/-)}$ mice did not have any problem uptaking chylomicron remnant by the liver which carries an extra TG after delivering it to the adipose tissue. HDMPPA retarded TC and TG accumulations in the vital organs. Thiobarbituric acid reactive substances (TBARS) in the brain and spleen were the highest and that in the testis were the lowest. Poly-unsaturated fatty acids in the brain and activated peroxisome in the spleen might be responsible for high TBARS levels in these organs. The greatest antioxidant effect of HDMPPA against lipid peroxidation was observed in the spleen, showing 21.47% decrease. The most noticeable effect of HDMPPA was observed in glutathione (GSH) level. GSH levels of six organs in the HDMPPA group were significantly higher than those of the control group. GSH-peroxidase activity was negatively related to GSH level of the organs except liver and spleen. In conclusion, HDMPPA from Korean cabbage kimchi inhibits the lipid accumulation as well as increases the antioxidant status in the vital organs of $apoE^{(-/-)}$ mice fed an atherogenic diet.

본 연구는 동맥경화를 억제하는 것으로 보고된 김치 활성성분인 HDMPPA가 동맥경화 식이를 섭취한 $apoE^{(-/-)}$ mice의 주요 장기(동맥제외)에서 지질 축적 억제 및 항산화 효과를 살펴보았다. $ApoE^{(-/-)}$ mice는 1% 콜레스테롤과 10% 라드를 함유한 합성식이를 8주간 섭취하면서 HDMPPA(1 mg/BW/day)를 복강 주사하였고 대조군은 동일양의 PBS를 주사하였다. 간, 신장, 비장, 폐, 고환 그리고 뇌 조직에서 콜레스테롤, 중성지질, TBARS, 총 GSH 농도 및 GSH-peroxidase 활성을 측정하였다. 대조군의 콜레스테롤 농도는 폐, 비장 그리고 뇌에서 높았으며 간이 가장 낮았는데 이러한 경향은 HDMPPA군에서도 동일하게 관찰되었으나 축적된 콜레스테롤 농도는 낮았다. HDMPPA의 콜레스테롤 축적 억제 효과는 간(22.65%, p<0.01), 비장(11.73%, p<0.05), 그리고 뇌(15.34%, p<0.05)에서 유의적으로 관찰되었다. 이에 반해 중성지질의 농도는 간, 신장 그리고 고환에서 높아 총콜레스테롤 축적과 다른 양상을 보였는데 이는 apo $E^{(-/-)}$ 마우스의 간이 중성지질을 함유한 지단백질을 받아들이는데는 문제가 없기 때문으로 생각된다. HDMPPA군의 간(14.7%, p<0.05), 신장(22.83%, p<0.001) 그리고 비장(35.87%, p<0.05)의 중성지질 농도는 유의적으로 낮았다. 지질과산화물 농도(TBARS)는 불포화지방산을 다량 함유한 뇌와 peroxisome이 발달하여 활성산소의 생성이 높은 비장에서 가장 높았으며 항산화물질을 다량 함유하고 있는 고환이 가장 낮았다. HDMPPA군의 비장의 TBARS 농도는 대조군에 비해 21.47%(p<0.01) 유의적으로 낮았다. GSH 농도는 고환, 간, 그리고 신장에서 높아 TBARS 결과와 반대였다. GSH-px 활성이 높은 장기의 GSH 농도는 낮은 편이었으나 간과 비장은 예외였다. HDMPPA군의 GSH 농도는 대조군에 비해 모든 장기에서 유의적으로 높았는데 이는 알려져 있는 HDMPPA의 항산화작용 때문으로 생각된다. 이상의 결과로부터 김치의 활성 성분인 HDMPPA는 동맥경화유발 식이를 섭취한 $apoE^{(-/-)}$ mice의 주요 장기에서 지질축적을 억제하며 체내 항산화 작용을 돕는 효과가 있음을 확인하였다.

Keywords

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