Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Change in Proteomic Profiles of Genetically Modified 1,3-Propanediol-Producing Recombinant E. coli

  • Jin, Li-Hua (Department of Chemical Engineering, Chosun University) ;
  • Lee, Jung-Heon (Department of Chemical Engineering, Chosun University)
  • Published : 2008.08.31
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Abstract

The recombinant E. coli $\Delta$6 mutant (galR, glpK, gldA, IdhA, lacI, tpiA) was used to produce 1,3-propanediol (PD) from glucose. The 1,3-PD production increased with feedback control of the glucose concentration using fed-batch fermentation. The maximum 1,3-PD concentration produced was 43 g/l after 60 h of fermentation. Glycerol production was minimized when controlling the glucose concentration at less than 1 g/l. The expression levels of seven enzymes related to the 1,3-PD production metabolism were compared during the cell growth phase and 1,3-PD production phase, and their expression levels all increased during 1,3-PD production, with the exception of alcohol dehydrogenase.

Keywords

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