Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Eisenia Bicyclis Extracts and Pill on Blood Glucose and Lipid Profile in Streptozotocin-Induced Diabetic Mice

당뇨 생쥐에서 대황 추출물 및 정제환의 혈당과 지질 상태 개선 효과

  • Cho, Sung-Hee (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Park, So-Young (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu)
  • 조성희 (대구가톨릭대학교 식품영양학과) ;
  • 박소영 (대구가톨릭대학교 식품영양학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과)
  • Published : 2008.09.30
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Abstract

The objectives of this study were to investigate the antihyperglycemic and hypolipidmic effects of sea oak(Eisenia bicyclis, EB) in the diabetic state and to examine the appropriateness of formulated EB pill for the effects. The various test materials obtained from EB were included in the experimental diets with 15% fat/0.5% cholesterol and fed to streptozotocin-induced diabetic mice weighing $35.0{\pm}0.7$ g for three weeks but not in the control diet having the same composition. The test materials were EB dry powder, water and ethanol extracts, viscozyme-treated EB water extract(EB enzyme-TR) and formulated pill containing dry powders of the EB, two kinds of seaweed, black soybean, sesame, onion and garlic. BG was measured during feeding period and serum insulin, lipids and thiobarbituric acid reactive substances(TBARS) and intestinal disaccharidase activities were measured at the end of the three weeks of the feeding. BG increase was lower in the EB enzyme-TR group after 10 days of the experimental diet but lower in EB pill group after 15 days compared with the control group. Serum insulin levels were higher in the EB enzyme-TR and EB pill groups. Intestinal maltase but not sucrase activity was higher in EB enzyme-TR fed group than the control group. Serum levels of total cholesterol and triglyceride were reduced by the EB enzyme-TR and EB pill compared with the control diet. HDL-/total cholesterol was increased by all EB test materials. Serum TBARS levels were lower in the EB ethanol extract and EB pill groups than in the control group and tended to be lower in the other EB groups. It is concluded that the EB enzyme-TR is the best among the EB preparations to be utilized as a functional component for improving blood glucose and lipid profile in diabetic subjects in the future. However, the pill containing low level of the EB powder is also regarded as effective and readily usable when formulated with the several other ingredients of the proper composition.(Supported by the RIC Program of MOCIE, Korea).

Keywords

References

  1. Korea National Statistical Office. Cause of death statistics; 2007
  2. Wildman RC. Handbook of Nutraceuticals and Functional Foods 2nd ed. CRC press; 2007
  3. Sung JH, Ha YS, Im MH, Im JG, Kang KS. Seaweed and health. In: Foods and Health. Seoul: Hyungseol Press Co.; 2002. p.190
  4. Lee JG, Lim YS, Joo DS, Joung IH. Effects of diet with seatangle (Kjellmaniella crassifolia) on calciumacsortion, serum composition and feces in rats. J Korean Fish Soc 2002; 35: 601-607
  5. Oh HK, Lim HS. Effects of the products of raw sea tangle on chronic idiopathic constipation. J Korean Soc Food Sci Nutr 2007; 36: 720-726 https://doi.org/10.3746/jkfn.2007.36.6.720
  6. Cho KJ, Lee YS, Ryu BH. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. J Kor Fish Soc 1990; 23: 345-352
  7. Kim YM, Do JR, Kim DS, Park JH. Cytotoxicities of hydrolyzed crude laminaran from Eisenia biscyclis on the SNU-1, Hela and SW cells. Korean J Food Sci Technol 2006; 38: 793-798
  8. Lee HS, Choi MS, Lee YK, Park SH, Kim YJ. A study on the development of high-fiber supplements for the diabetic patients (2)-Effect of seaweed supplementation on the lipid and glucose metabolism in streptozotocin-induced diabetic rats. Korean J Nutr 1996; 29: 296-306
  9. Lee KS, Seo JS, Choi YS. Effect of sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats. J Korean Soc Food Sci Nutr 1998; 27: 960-967
  10. Kim HS, Kim GA. Effects of the feeding Hijika fusi-forme (Harvey) Okamura on lipid composition of serum in dietary hyperlipidemic rats. J Korean Soc Food Sci Nutr 1998; 27: 718-723
  11. Joo DS, Lee JK, Choi YS, CHo SY, Je YK, Choi JW. Effects of seatangle oligosaccharide drink on serum and hepatic lipids in rats fed a hyperlipidemic diet. J Korean Soc Food Sci Nutr 2003; 32: 1364-1369 https://doi.org/10.3746/jkfn.2003.32.8.1364
  12. Ruberto G, Baratta MT, Biondi DM, Amico V. Antioxidant activity of extracts of the marine algal genus Cystoseira in a micellar model system. J Appl Phycol 2001; 13: 403-407 https://doi.org/10.1023/A:1011972230477
  13. Cho SH, Kang SE, Cho JY, Kim AR, Park SM, Hong YK, Ahn DH. The antioxidant properties of brown seaweed (sargassum siliquastrum) extracts. J Med Food 2007; 10: 479-485 https://doi.org/10.1089/jmf.2006.099
  14. Kang HS, Kim HR, Byun DS, Son BW, Nam TJ, Choi JS. Tyrosinase inhibitors isolated from the edible brown alga Ecklonia stolonifera. Arch Pharm Res 2004; 27: 1226-1232 https://doi.org/10.1007/BF02975886
  15. Jang YH, Choi SW, Cho SH. Effect of Eisenia bicyclis and its pill on serum lipid status in rats fed high fat diet. Korean J Nutr 2008; 41: 5-12
  16. Kim YM, Han CK, Bang SJ, Park JH. Effect of laminaran from Eisenia bicyclis on serum lipids in rats fed high choelsterol diet. J Korean Soc Food Sci Nutr 2006; 36: 841-846 https://doi.org/10.3746/jkfn.2006.35.7.841
  17. Cahyana AH, Shuto Y, Kinoshita Y. Pyropheophytin a as an antioxidative substance from the marine alga, Arame (Eisenia bicyclis). Biosci Biotech Biochem 1992; 56: 1533-1535 https://doi.org/10.1271/bbb.56.1533
  18. Okada Y, Ishimaru A, Suzuki R, Okuyama T. A new phloroglucinol derivatives from the brown alga Eisenia bicyclis: Potential for the effective treatment of diabetic complications. J Nat Prod 2004; 67: 103-105 https://doi.org/10.1021/np030323j
  19. Like AA, Rossini AA. Streptozotocin-induced pancreatic insulitis: new model of diabetic mellitus. Science 1976; 193: 415-417 https://doi.org/10.1126/science.180605
  20. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc committee on the reformation of the AIN-76A rodent diet. J Nutr 1993; 123: 1939-1951 https://doi.org/10.1093/jn/123.11.1939
  21. Cho SH, Lee JY, Choi YS, Choi KH. Dietary Effects of Fiber Produced from Gluconoacetobacter hansenii on Digestive tract and lipid metabolism in rats. J Korean Soc Food Sci Nutr 2002; 31: 802-807 https://doi.org/10.3746/jkfn.2002.31.5.802
  22. Dahlqvist A. Assay of intestine disaccharidases. Scand J Chin Lab Invest 1984; 44: 169-172 https://doi.org/10.3109/00365518409161400
  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-275
  24. Yagi K. Microdetermination of lipoperoxide in blood. Biochem Med 1976; 15: 212-216 https://doi.org/10.1016/0006-2944(76)90049-1
  25. Kousaka K, Ogi N, Akazawa Y, Fujieda M, Yamamoto Y, Tanaka Y, Kimura J. Novel oxylipin metabolites from the brown alga Eisenia bicyclis. J Nat Prod 2003; 66: 1318-1323 https://doi.org/10.1021/np030049t
  26. Choi Y, Cho SH, Kim HJ, Lee HJ. Effects of soluble fibers on lipid metabolism and acitivities of intestinal disaccharidases in rats. J Nutr Sci Vitaminol 1998; 44: 591-600 https://doi.org/10.3177/jnsv.44.591
  27. Jeong HJ, Sung HY, Choi YS, Cho SH. Effects of fructans on blood glucose, activities of disaccharidases and immune function in streptozotocin-induced diabetic mice. J Korean Soc Food Sci Nutr 2005; 34: 1188-1194 https://doi.org/10.3746/jkfn.2005.34.8.1188
  28. Andrase-Cetto A, Becerra-Jimenez J, Cardenas-Vazquez R, Alfaglucosidase- inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes. J Ethnopharmacol 2008; 116: 27-32 https://doi.org/10.1016/j.jep.2007.10.031
  29. Her BY, Sung HY, Choi YS. Ologosaccharide-supplemented soy ice cream or diabetic patients: Quality charateristics and effects on blood sugar and lipids in streptozotocin-induced diabetic rats. J Korean Nutr 2005; 38: 663-671
  30. Narendhirakannan RT, Subramanian S, Kandaswamy M. Biochemical evaluation of antidiabetogenic properties of some commonly used Indian plants on streptozotocin-induced diabetes in experimental rats. Clin Exp Pharmacol Physiol 2006; 33: 1150-1157 https://doi.org/10.1111/j.1440-1681.2006.04507.x
  31. Singab AN, El-Beshbishy HA, Yonekawa M, Nomura T, Fukai T. Hypoglycemic effect of Egyptian Morus alba root bark extract: effect on diabetes and lipid peroxidation of streptozotocin-induced diabetic rats. J Ethnopharmacol 2005; 100: 333-338 https://doi.org/10.1016/j.jep.2005.03.013
  32. Rchid H, Chevassus H, Nmila R, Guiral C, Petit P, Chokairi M, Sauvaire Y. Nigella sativa seed extracts enhance glucose-induced insulin release from rat-isolated Langerhans islets. Fundam Clin Pharmacol 2004; 18: 525-529 https://doi.org/10.1111/j.1472-8206.2004.00275.x
  33. Govindarajan R, Vijayakumar M, Rao ChV, Pushpangadan P, Asare-Anane H, Persaud S, Jones P, Houghton PJ. Antidiabetic activity of Croton klozchianus in rats and direct stimulation of insulin secretion in-vitro. J Pharm Pharmacol 2008; 60: 371-376 https://doi.org/10.1211/jpp.60.3.0013
  34. Ko BS, Jwon DY, Hong SM, Park S. In vitro anti-diabetic effects of crude extracts of Platycodi radix. Korean J Food Sci Technol 2007; 39: 701-707
  35. Kamgang R, Mboumi RY, Fondjo AF, Tagne MA, N'dille GP, Yonkeu JN. Antihyperglycaemic potential of the water-ethanol extract of Kalanchoe crenata (Crassulaceae). Nat Med 2008; 62; 34-40
  36. Li W, Dai RJ, Yu YH, Li L, Wu CM, Luan WW, Meng WW, Zhang XS, Deng YL. Antihyperglycemic effect of Cephalotaxus sinensis leaves and GLUT-4 translocation facilitating activity of its flavonoid constituents. Biol Pharm Bull 2007; 30: 1123-1129 https://doi.org/10.1248/bpb.30.1123
  37. Jeong HJ. Effect of chicory inulin and oligosaccharides on blood glucose, lipid metabolism and immune functions in streptozotocininduced diabetic mice [MS Thesis]. Daegu: Daegu University; 2003
  38. Heo SJ, Lee KW, Song CB, Jeon YJ. Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 2003; 18: 71-81 https://doi.org/10.4490/ALGAE.2003.18.1.071
  39. Kim EK, Lee SJ, Lim BO, Jeon YJ, Song MD, Park TK, Lee KH, Kim BK, Lee SR, Moon SH, Jeon BT, Park PJ. Antioxidative and neuroprotective effects of enzymatic extracts from leaves of perilla frutescens var. japonica. Food Sci Biotech 2008; 17: 279-286
  40. Cha JY, Shin HS, Cho YJ, Kim CT, Kim CJ, Influence of extrusion on the solubility of defatted soybean flour in enzymatic hydrolysis. Food Sci Biotech 2007; 16: 543-548