Histologic Evaluation of Collagen Generation in Mouse Subcutaneous Tissue Using 880 nm & 630 nm LED

  • Ahn, Jin-Chul (Medical Laser and Device Research Center, Dankook University) ;
  • Chung, Phil-Sang (Medical Laser and Device Research Center, Dankook University) ;
  • Chang, So-Young (Medical Laser and Device Research Center, Dankook University) ;
  • Hwang, Hee-Jun (Medical Laser and Device Research Center, Dankook University) ;
  • Shin, Jang-In (Medical Laser and Device Research Center, Dankook University) ;
  • Rhee, Chung-Ku (Medical Laser and Device Research Center, Dankook University)
  • Published : 2008.09.30

Abstract

We compared the clinical efficacy of LED therapy using 880 nm and 630 nm LED to test collagen accumulations in subcutaneous tissue of mouse after LED irradiation by measuring the quantity of collagen. 880 nm and 630 nm LED was irradiated on the back of ICR mouse given at $10.8J/cm^2$ followed for 30 minutes everyday for 5 weeks. Histological observation was performed by Hematoxylin & Eosin staining and Masson's Trichrome collagen staining. We also used Sircol soluble collagen assay kit for measuring the amounts of collagen in the mouse skin tissue after 1, 3, and 5 weeks post LED irradiation, respectively. Collagen generation was found at subcutaneous tissue, and the quantity of collagen in 880 nm LED group had grown more than that of 630 nm LED group at 5 weeks follow-up later. About 75% more efficacies for collagen generation were found in the group of 5th week of 880nm LED irradiation. The efficacy of 880nm LED could be more useful than 630 nm LED for synthesizing collagens in mouse subcutaneous tissue as time followed.

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