Comparative Analysis of the Amino Acids and Effective Components from Viola mandshurica

자화지정(Viola mandshurica)의 아미노산과 유용성분 비교 분석

  • Lee, Yang-Suk (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University)
  • 이양숙 (대구한의대학교 한방생약자원학과) ;
  • 김남우 (대구한의대학교 한방생약자원학과)
  • Published : 2008.10.31

Abstract

This study compared amino acids and effective components of Viola mandshurica produced in Korea with those of Chinese plants, as part of a study on nutritional andfunctional materials, to aid in the development of a valuable food. The contents of reducing sugar, free sugar, polyphenols, and soluble protein in the flowers of V. mandshurica were higher than in other fractions, being 10,485.11 mg%, 118.85 mg%, 1,259.26 mg%, and 23.67 mg%, respectively. Total flavonoid content of Chinese leaves was the highest at 2,370.75 mg%, followed by 1,844.43 mg% in Korean leaves. Free amino acids were highest in Korean leaves (5,345.21 mg%), followed by roots (4,692.24 mg%), flowers (3,807.87 mg%), and leaves of China plants (3,613.81 mg%). Arginine accounted for 55% of free amino acids in the roots, and all plant fractions contained more valine, aspartic acid, glutamic acid, alanine, and arginine, than other amino acids. The total content of amino acid derivatives was the highest (9,058.80 mg%) in Korean leaves. Especially, $\alpha$-aminoadipic acid constituted over 50% of total amino acid derivatives in all plant parts of V. mandshurica. When mineral contents were analyzed, leaves grown in China showed the highest content of 5,917.57 mg%, and the contents of K, Ca, and Mg were much greater than those of other minerals, together comprising over 95% of total mineral content in leaves and flowers from Korea.

본 연구는 한방생약재로 사용되고 있는 자화지정의 식품학적 가치와 식품소재로서의 개발 가능성을 검토하기 위하여 국내에서 채집된 자화지정의 기원식물인 제비꽃과 한방재료로 유통, 판매되고 있는 중국산 자화지정을 비교, 분석하였다. 분석 결과 자화지정 꽃의 환원당과 유리당이 각각 10,485.11 mg%와 118.85 mg%로 가장 많았으며, 수용성 단백질과 폴리페놀의 함량도 각각 1,259.26 mg%와 2366.67 mg%로 함량이 높은 것으로 확인되었다. 플라보노이드 함량은 중국산 잎에서 2,370.75 mg%이었으며, 잎은 1,844.43 mg%을 함유하였다. 유리아미노산은 잎(5,345.21 mg%) > 뿌리(4,692.24 mg%) > 꽃(3,807.87 mg%) > 중국산 잎 (3,613.81 mg%)의 순으로 특히 뿌리에는 arginine의 함량이 전체의 55 %이상 차지하였으며 모든 부위에서 valine, aspartic acid, glutamic acid, alanine, arginine 등의 함량이 비교적 높은 것으로 나타났다. 아미노산 유도체는 잎에서 9,058.80 mg%로 가장 많이 함유하였으며 $\alpha$-aminoadipic acid는 모든 부위에서 50% 이상을 함유하였으며 특히 잎에서는 아미노산 유도체(7,124.23 mg%) 중 약 80%를 차지하였다. 무기질 함량은 중국산 잎이 5,917.57 mg%로 가장 높았으며, 모든 부위에서 K, Ca와 Mg의 함량이 매우 높았으며, 특히 잎과 꽃의 95% 이상을 차지하였다. 이상의 결과 국내에서 채집된 자화지정의 잎이 중국산 잎보다는 환원당과 폴리페놀, 플라보노이드 그리고 무기질의 함량은 낮았으나 수용성 단백질과 아미노산을 다량 함유하였으며, 국내에서 채집된 자화지정의 뿌리와 꽃에서도 유용한 유용성분들을 다량 함유하므로 음료나 건강보조식품 등 다양한 기능성 제품 개발에 이용될 수 있는 한방약용자원인 것으로 사료된다.

Keywords

References

  1. Chang, K.M. (2007) A study cookery utilization of Pimpinella brachycarpa N. for developing as functional food. Korean J. Food Culture, 22, 274-282
  2. Kim, K.S. (2007) Functional ingredient compositions of soybean curds(Tufu) made with black soybeans(Huktae) and white soybeans(Baktae). Korean J. Food and Nutr., 20, 158-163
  3. 이시진. (1983) 본초강목. 서울, 고문사, p647
  4. 신민교. (1986) 원색임상본초학. 서울, 남산당, p340
  5. 江蘇新醫學院. (1999) 中藥大辭典. 上海, 上海科學技術出版社, p800-802
  6. KHP. (2007) The korean herbal pharmacopoeia. KFDA, p294
  7. 河南省衛生廳編. (1974) 河南省中藥村?制規範. 河南省, 河南科學技術出版社, p365
  8. 中醫大辭典編輯委員會編. (1982) 中醫大辭典 (中象分冊). 北京, 人民衛生出版社, p362
  9. 成都中醫學院主編. (1982) 中象學, 上海, 上海科學技術出版社, p78
  10. Yook, C.S., Lee, W.C. and Moon, C.K. (1989) Studies on flavonoid glycoside of the leaves of Viola diamantica. Yakhak Hoeji, 33, 124-128
  11. 문관심. (1984) 약초의 성분과 이용. 일월서각. pp481-482
  12. 6박성규, 안덕균. (1991) 자화지정 종류의 효능에 관한 비교 연구. 본초분과학회지, 6, 77-83
  13. Ko, W.C. and Shin, M.K. (1987) Experimental studies on the analgesic and antiphlogistic effects of Violae herba. Kor. J. Pharmacogn., 18, 210-215
  14. Lee, B.W. and Kim, D.H. (2003) Inhibitory effect of Violae herba extract on inflammatory cytokine production by IL-1$\beta$ and TNF-$\alpha$ in cultured human synovial cells. Kor. J. Herbology, 18, 89-96
  15. Yun, J.S., Chung, B.H., Kim, N.Y., Seong, N.S., Lee, H.Y., Lee, J.H. and Kim, J.D. (2003) Screening of 94 plant species showing ACE inhibitory activity. Korean J. Medicinal Crop Sci., 11, 246-251
  16. Witkowska-Banaszczak, E., Bylka, W., Matlawska, I., Goslinska, O. and Muszynski, Z. (2005) Antimicrobial activity of Viola tricolor herb. Fitoterapia., 76, 458-461 https://doi.org/10.1016/j.fitote.2005.03.005
  17. Moon, H.I., Lee, J., Zee, O.P. and Chung, J.H. (2005) The effect of flavonol glycoside on the expression of matrix metalloproteinase-1 in ultraviolet-irradiated cultured human skin fibroblasts. J. Ethnopharmacol., 101, 176-179 https://doi.org/10.1016/j.jep.2005.04.032
  18. Moon, H.I., Jung, J.C. and Lee, J. (2006) Aldose reductase inhibitory effect by tectorigenin derivatives from Viola hondoensis. Bioorg. Med. Chem., 14, 7592-7594 https://doi.org/10.1016/j.bmc.2006.07.002
  19. Moon, C.K. and Yook, C.S. (1981) Ein flavonol- triglykosid aus herba Viola japonica. Kor. J. Pharmacogn., 12, 146
  20. AOAC. (2005) Official method of analysis. 18th ed., Association of official analytical chemists. Washington D.C. USA. 45, 21-22
  21. Nelson, N. (1944) A photometric adaption of the somogyi method for determination of glucose. J. Biol. Chem., 153, 375-381
  22. Shim, K.H., Sung, N.K., Choi, J.S. and Kang, K.S. (1989) Changes in major components of japanese apricot during ripening. J. Korean Soc. Food Nutr., 18, 101-108
  23. Lowry, O.H., Roserbrough, N.J., Farr, A.L. and Randall, RJ. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265
  24. Swain, T., Hillis, W.E. and Ortega, M. (1959) Phenolic constituents of Ptunus domestica I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric., 10, 83-88
  25. Nieva Moreno, M.I., Isla, M.I., Sampietro, A.R. and Vattuone, M.A. (2000) Comparison of the free radical- scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol., 71, 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  26. Yun, S.I., Choi, W.J., Choi, Y.D., Lee, S.H., Yoo, S.H., Lee, E.H. and Ro, H.M. (2003) Distribution of heavy metals in soils of Shihwa tidal freshwater marshes. Korean J. Ecol., 26, 65-70 https://doi.org/10.5141/JEFB.2003.26.2.065
  27. Hwang, J.B., Yang, M.O. and Shin, H.K. (1997) Survey for approximate composition and mineral content of medicinal herbs. Korean J. Food Sci. Technol., 29, 671-679
  28. Joo, E.Y. and Kang, W.J. (2005) Analysis on the components of the Angelica dahurica root. Korean J. Food Preserv., 12, 476-481
  29. Lee, Y.S. (2007) Antioxidative and physiological activity of the Angelica dahurica leaves extracts. Korean J. Food Preserv., 14, 74-86
  30. Yang, H.C., Heo, N.C., Choi, K.C. and Ahn, Y.J. (2007) Nutritional composition of white-flowered and pink- flowered lotus in different parts. Korean J. Food Sci. Technol., 39, 14-19
  31. Shin, S.R. (1999) Studies on the nutritional components of dandelion (Taraxacum officinale). Korean J. Postharvest Sci. Technol., 6, 495-499
  32. Kim, E.Y., Baik, I.H., Kim, J.H., Kim, S.R. and Rhyu, M.R. (2004) Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol., 36, 333-338
  33. Lee, S.O., Lee, H.J., Yu, M.H., Im, H.G. and Lee, I.S. (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol., 37, 233-240
  34. Lee, J.H. and Lee, S.R. (1994) Analysis of phenolic substances content on Korea plant foods. Korea J. Food Sci. Technol., 26, 310-316
  35. Solms, J. (1969) The taste of amino acids, peptides, and proteins. J. Agric. Food Chem., 17, 686-688 https://doi.org/10.1021/jf60164a016
  36. Mau, J.L., Chyau, C.C., Li, J.Y. and Tseng, Y.H. (1997) Flavor components in straw mushrooms Volariella volvacea harvested at different stages of maturity. J. Agric. Food Chem., 45, 4726-4729 https://doi.org/10.1021/jf9703314
  37. Kang, M.H., Seo, Y.H., Kim, J.B., Shin, S.R. and Kim, K.W. (2000) The chemical composition of Taraxacum officinale consumed in Korea. Korean J. Soc. Food Sci., 16, 182-187
  38. Kim, O.K., Kung, S.S., Park, W.B., Lee, M.W. and Ham, S.S. (1992) The nutritional components of aerial whole plant and juice of Angelica keiskei Koidz. Korean J. Food Sci. Technol., 24, 592-596
  39. Lee, S.T., Park, J.M., Lee, H.K., Kim, M.B., Cho, J.S. and Heo, J.S. (2000) Component comparison in different growth stages and organs of Saururus chinensis Baill. Korean J. Med. Crop Sci., 8, 312-318