Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Changes in Enzyme Activity and Physiological Functionality of Doenjang (Soybean Paste) Prepared with Extracts of Phellinus linteus

상황버섯 추출액을 이용하여 제조한 된장의 효소 활성 및 기능성의 변화

  • Rhee, Chang-Ho (Gyeoungbuk Institute for Bio Industry) ;
  • Kim, Byoung-Soo (School of Food Science & Biotechnology, Andong National University) ;
  • Shin, Mi-Kyoung (School of Food Science & Biotechnology, Andong National University) ;
  • Woo, Cheol-Joo (Department of Food science & Technology, Kyungpook National University) ;
  • Kim, Jung-Hee (Department of Food & Beverage Culinary, Kyungdong College Techno-information) ;
  • Kwon, Ki-Young (Farming Union of Andong Shangwhang Meju) ;
  • Park, Heui-Dong (Department of Food science & Technology, Kyungpook National University)
  • 이창호 ((재)경북바이오산업연구원) ;
  • 김병수 (안동대학교 식품생명공학과) ;
  • 신미경 (안동대학교 식품생명공학과) ;
  • 우철주 (경북대학교 식품공학과) ;
  • 김정희 (경동정보대학 식음료조리과) ;
  • 권기영 (안동상황메주영농조합) ;
  • 박희동 (경북대학교 식품공학과)
  • Published : 2008.10.31
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Abstract

To evaluate changes in functional characteristics of traditional Doenjang during aging, Doenjang was prepared using an extract of Phellinus linteus (Phellinus extract). Control Doenjang was aged without the extract. The protease activity of Doenjang prepared with Phellinus extract was 3.15 units/mL. Tyrosinase and acetylcholinesterase inhibition activities were 45.78% and 55.18% of control, respectively, in the treated sample. When Salmonella enterica serovar Typhimurium TA100 was used as a reporter strain, antimutagenic activities against the mutagens MNNG and NPD were 90.42% and 82.57% of control values in the treated sample. When S. enterica serovar Typhimurium TA98 was used, antimutagenic activities were 60.28% and 50.33% of control, respectively. Hydrogen-donating activity was 86.65% in the treated sample, which was higher than that of the control (61.69%). Daidzin (an isoflavon glucoside) levels in Doenjang prepared with Phellinus extract were higher, by 35.49 mg/kg, than the control, whereas genistin was not detected in either group. Daidzin and genistin aglycone levels were 263.01 mg/kg and 262.60 mg/kg in the control and test groups, respectively.

전통 된장의 숙성기간 중 기능적 특성의 변화를 조사하기 위하여 상황버섯 추출물을 이용하여 된장을 담금하여 일정기간 숙성한 후, 된장의 protease 활성, 항돌연변이 활성, isoflavone 등을 측정하였다. Protease의 활성은 상황버섯 추출물을 이용한 실험구가 3.15 unit/mL로 대조구보다 높은 활성을 나타내었으며 tyrosinase의 저해 활성과 ACE 저해 활성은 실험구가 각각 45.78%와 55.18%로 대조구보다 약 20% 이상 높은 활성을 나타내었다. 항돌연변이 활성은 실험구가 S. enterica serovar Typhimurium TA100을 사용한 경우 변이원 MNNG와 NPD에 대한 항돌연변이 활성은 각각 90.42%와 82.57%, S. enterica serovar Typhimurium TA98을 사용한 경우 변이원 NPD와 NQO에 대한 항돌연변이 활성은 각각 60.28%와 50.33%로 대조구보다 높은 활성을 나타내었다. 수소 공여능은 실험구의 활성이 86.65%로 대조구의 61.69%보다 높게 나타났다. 상황버섯 추출물을 이용하여 제조한 된장의 isoflavon 중 glucoside인 daidzin은 실험구가 35.49 mg/kg높게 나타났으며 genistin은 실험구와 대조구 모두에서 검출되지 않았다. Aglycone인 daidzein과 genistein은 각각 263.01 mg/kg와 262.60 mg/kg로 대조구 보다 높은 함량을 나타내었다.

Keywords

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