Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Proteomic Characterization of the 'Agakong', a Small-seeded Recombinant Inbred Line Derived from 'Eunhakong' (Glycine max) $\times$ 'KLG10084' (Glycine soja)

  • Choi, Ung-Kyu (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Ryu, Hyun-Su (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Hyun-Tae (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Yun, Sun-Mi (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Su-Jin (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Choi, Jae-Dek (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Hwang, Young-Hyun (Department of Agronomy, Kyungpook National University) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kwon, Oh-Shin (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Published : 2008.10.31
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Abstract

This study was conducted to identify the differences in proteomic characteristics of 'Agakong', recombinant inbred line, and its parental genotypes 'Eunhakong' (Glycine max) and 'KLG10084' (G. soja). The isoflavone content of 'Agakong' was 3 times higher than that of its parental lines. A combined high-throughput proteomic approach was employed to determine the expression profile and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of proteins are quite similar, but lots of protein spot intensities varied among the genotypes. A total of 41 proteins, representing significant difference in the quantities of protein among the lines, were successfully identified. Among them, more than 50% of the proteins identified were subunits of glycinin and $\beta$-conglycinin, 2 major storage proteins. This study showed that the proteomic analysis could help to define specific changes in protein level and composition, which can occur in the generation of new soybean varieties.

Keywords

References

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